LOCUS V025578 6128 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025578 VERSION V025578 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..1618 /label="CD79A(NM_001783)" /note="CD79A(NM_001783)" /gene="CD79A" CDS 1625..1690 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1728..1952 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1998..2426 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2440..2769 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2836..3630 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3804..3925 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3974..3990) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3998..4014 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4022..4052) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4067..4088 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4376..4961) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5132..5992) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5993..6097) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgcctgggg gtccaggagt 961 cctccaagct ctgcctgcca ccatcttcct cctcttcctg ctgtctgctg tctacctggg 1021 ccctgggtgc caggccctgt ggatgcacaa ggtcccagca tcattgatgg tgagcctggg 1081 ggaagacgcc cacttccaat gcccgcacaa tagcagcaac aacgccaacg tcacctggtg 1141 gcgcgtcctc catggcaact acacgtggcc ccctgagttc ttgggcccgg gcgaggaccc 1201 caatggtacg ctgatcatcc agaatgtgaa caagagccat gggggcatat acgtgtgccg 1261 ggtccaggag ggcaacgagt cataccagca gtcctgcggc acctacctcc gcgtgcgcca 1321 gccgcccccc aggcccttcc tggacatggg ggagggcacc aagaaccgaa tcatcacagc 1381 cgaggggatc atcctcctgt tctgcgcggt ggtgcctggg acgctgctgc tgttcaggaa 1441 acgatggcag aacgagaagc tcgggttgga tgccggggat gaatatgaag atgaaaacct 1501 ttatgaaggc ctgaacctgg acgactgctc catgtatgag gacatctccc ggggcctcca 1561 gggcacctac caggatgtgg gcagcctcaa cataggagat gtccagctgg agaagccgct 1621 cgaggactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga 1681 cgatgacaag tagtgagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag 1741 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 1801 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 1861 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 1921 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 1981 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 2041 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 2101 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 2161 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 2221 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 2281 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 2341 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 2401 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 2461 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 2641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 2701 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 2761 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga 2821 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 2881 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 2941 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 3001 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 3061 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 3121 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 3181 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 3241 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 3301 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 3361 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 3421 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3481 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3541 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3601 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3661 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3721 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3781 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 3841 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 3901 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 3961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 4021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 4081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 4141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 4201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 4261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 4321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 4381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 4441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 4501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 4561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 4621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 4681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 4741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg 4921 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 4981 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 5041 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 5101 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 5161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 5221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 5281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 5341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 5401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 5461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 5521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 5581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 5641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 5701 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 5761 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 5821 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 5881 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 5941 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 6001 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 6061 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 6121 ctgacgtc //