LOCUS V033794 6602 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V033794 VERSION V033794 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 938..961 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" CDS 968..2089 /label="CCR6(NM_004367)" /note="CCR6(NM_004367)" /gene="CCR6" polyA_signal 2169..2393 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2439..2867 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2881..3210 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3277..4071 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4245..4366 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4415..4431) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4439..4455 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4463..4493) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4508..4529 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4817..5402) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5573..6433) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6434..6538) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tgaagaccat 901 catcgccctc agctacatct tttgtctggt cttcgcggat tacaaggatg acgacgataa 961 gggatccatg agcggggaat caatgaattt cagcgatgtt ttcgactcca gtgaagatta 1021 ttttgtgtca gtcaatactt catattactc agttgattct gagatgttac tgtgctcctt 1081 gcaggaggtc aggcagttct ccaggctatt tgtaccgatt gcctactcct tgatctgtgt 1141 ctttggcctc ctggggaata ttctggtggt gatcaccttt gctttttata agaaggccag 1201 gtctatgaca gacgtctatc tcttgaacat ggccattgca gacatcctct ttgttcttac 1261 tctcccattc tgggcagtga gtcatgccac cggtgcgtgg gttttcagca atgccacgtg 1321 caagttgcta aaaggcatct atgccatcaa ctttaactgc gggatgctgc tcctgacttg 1381 cattagcatg gaccggtaca tcgccattgt acaggcgact aagtcattcc ggctccgatc 1441 cagaacacta ccgcgcagca aaatcatctg ccttgttgtg tgggggctgt cagtcatcat 1501 ctccagctca acttttgtct tcaaccaaaa atacaacacc caaggcagcg atgtctgtga 1561 acccaagtac cagactgtct cggagcccat caggtggaag ctgctgatgt tggggcttga 1621 gctactcttt ggtttcttta tccctttgat gttcatgata ttttgttaca cgttcattgt 1681 caaaaccttg gtgcaagctc agaattctaa aaggcacaaa gccatccgtg taatcatagc 1741 tgtggtgctt gtgtttctgg cttgtcagat tcctcataac atggtcctgc ttgtgacggc 1801 tgcaaatttg ggtaaaatga accgatcctg ccagagcgaa aagctaattg gctatacgaa 1861 aactgtcaca gaagtcctgg ctttcctgca ctgctgcctg aaccctgtgc tctacgcttt 1921 tattgggcag aagttcagaa actactttct gaagatcttg aaggacctgt ggtgtgtgag 1981 aaggaagtac aagtcctcag gcttctcctg tgccgggagg tactcagaaa acatttctcg 2041 gcagaccagt gagaccgcag ataacgacaa tgcgtcgtcc ttcactatgt gagaattctg 2101 cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc 2161 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 2221 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 2281 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 2341 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 2401 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 2461 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 2521 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 2581 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 2641 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 2701 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 2761 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 2821 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 2881 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2941 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 3001 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 3061 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 3121 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 3181 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 3241 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 3301 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 3361 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 3421 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 3481 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 3541 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 3601 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 3661 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 3721 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 3781 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 3841 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 3901 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 3961 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 4021 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 4081 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 4141 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 4201 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 4261 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 4321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 4381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 4441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 4501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 5281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5341 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 5401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 5461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 5521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 5581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 5641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 5701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 5761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 5821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 5881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 5941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 6001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 6061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 6121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 6181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 6241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 6301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 6361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 6421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 6481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 6541 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg 6601 gt //