LOCUS       V035716                10750 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035716
VERSION     V035716
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             964..6240
                     /label="CASZ1(NM_001079843)"
                     /note="CASZ1(NM_001079843)"
                     /gene="CASZ1"
     CDS             6247..6312
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    6350..6574
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6620..7048
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7062..7391
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7458..8252
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8426..8547
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8596..8612)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8620..8636
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8644..8674)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8689..8710
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8998..9583)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9754..10614)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10615..10719)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggatc ttggaacagc tgagggcacc cggtgcacgg acccgcctgc aggcaagccc
     1021 gccatggcgc ccaaacgcaa gggtggcctg aagctgaacg ccatctgcgc caagctgagc
     1081 cgccaggtgg tggtggagaa gcgagctgac gccggctccc acacggaggg cagcccatcg
     1141 cagccccggg accaagagcg cagtggccct gagtctgggg cagcccgggc cccccgcagc
     1201 gaggaagaca agagacgggc agtgatcgag aagtgggtga acggggagta cagcgaggag
     1261 ccggcaccca cacccgtgtt ggggcggatt gcccgcgagg gcctggagct gcctcccgag
     1321 ggtgtctaca tggtgcagcc ccaggggtgc agcgatgagg aagaccacgc ggaggagccc
     1381 tccaaggacg gcggtgccct ggaggagaag gattcggacg gggcagcctc caaggaggac
     1441 agcggcccca gcaccaggca ggcttcagga gaggcctcct cgctgcggga ctacgcggcc
     1501 tccaccatga ccgagttcct cggcatgttt ggctatgatg accagaacac gcgggacgag
     1561 ctggccagga agatcagctt tgagaagctg cacgcgggct ccaccccgga ggcagccacc
     1621 tcctccatgc tgcccacctc cgaggatacc ctcagcaagc gggcgcggtt ctctaagtat
     1681 gaggagtaca tccgcaagct caaggctggc gagcagctct cctggccggc ccccagcacc
     1741 aagaccgagg agcgggtggg caaggaggtg gtgggcaccc tgcccggcct gcggctgccc
     1801 agcagcacgg cccacctgga gaccaaggcc accatcctgc ccctgccgtc gcacagcagt
     1861 gtccagatgc agaacctggt agcccgggcc tccaagtacg acttcttcat ccaaaaactg
     1921 aagaccggcg agaatctgcg gccccagaac gggagcacct acaagaagcc atccaagtac
     1981 gacctggaga atgtcaagta cctgcacctc ttcaaacccg gggagggcag ccccgacatg
     2041 ggcggggcca tcgccttcaa gacaggcaag gtggggcgcc cttccaagta cgacgtccgg
     2101 ggcatccaga agccaggccc cgccaaggtt ccgcccaccc ccagcctggc tcccgcaccc
     2161 ctcgccagcg tgcccagtgc ccccagcgcc cccgggccag ggccagagcc tcctgcctcc
     2221 ctgtccttca acactcccga gtacctgaag tcaaccttct ccaaaacaga ctccatcacc
     2281 acggggaccg tctccactgt caagaacgga ctgcccacag ataaaccagc cgtcactgaa
     2341 gatgtaaaca tttaccagaa atatattgcc aggttctcgg gcagccagca ctgtggccac
     2401 atccactgtg cctaccagta ccgcgagcac taccactgcc ttgaccctga gtgtaactac
     2461 cagaggttca cgagtaagca ggacgtgatc cgccactaca acatgcacaa gaagcgcgac
     2521 aactccctgc agcacggctt catgcgtttc agcccgctgg acgactgcag cgtctactac
     2581 cacggctgcc acctcaatgg gaagagcacc cactatcact gcatgcaggt gggctgtaac
     2641 aaggtgtaca cgagcacgtc tgacgtgatg acccacgaga acttccacaa gaagaatacc
     2701 cagctcatta acgacggctt ccagcgcttc cgagccaccg aagactgtgg cacagccgac
     2761 tgccagttct acggacagaa gaccacgcac ttccactgca ggcgccccgg ctgcacattc
     2821 actttcaaga acaagtgtga catcgagaag cacaagagct accacatcaa ggacgatgcc
     2881 tacgccaagg acggcttcaa gaagttctac aagtacgagg agtgcaagta cgagggctgc
     2941 gtgtacagca aggctaccaa ccacttccac tgcatccgcg ccggctgcgg cttcaccttc
     3001 acctccacca gccagatgac ctctcacaag cgcaagcatg agcgccggca catccgctcc
     3061 tcgggcgcgc tggggctgcc gccctcgctg ctgggcgcca aggacacgga gcacgaggag
     3121 tccagcaacg acgaccttgt tgacttctcc gccctgagca gcaagaactc cagcctgagc
     3181 gcctccccta ccagccagca gtcctctgcg tccctggctg ccgccactgc cgccaccgag
     3241 gctgggccca gtgccaccaa acctcccaac agcaagatct cggggctgct gccccagggc
     3301 ctgcctggct caatccccct ggccctggcc ctctccaact cgggcctgcc cacccccacg
     3361 ccctacttcc ccatactggc tggccgtggg agcacctccc tgcctgtggg cacccccagc
     3421 ctcctgggtg ccgtgtcgtc tgggtcagca gcctcagcca cccctgacac acccacgctg
     3481 gtcgcctcgg gagctggaga ctcagccccc gtggctgccg cctctgtccc ggcaccaccc
     3541 gcctccatca tggagaggat ctctgcaagc aagggcctca tctcgcccat gatggccagg
     3601 ctggctgcag ctgccctcaa gccctctgcc acctttgacc caggaagcgg gcagcaggtc
     3661 accccagcca ggttcccccc ggcccaagtg aagccggaac ccggtgagag caccggcgcc
     3721 ccaggccccc acgaagcctc ccaggaccgc agtctagacc tgactgtgaa ggagcccagc
     3781 aacgaatcaa atggccacgc agtcccggca aattcatctc ttttatcctc gcttatgaat
     3841 aagatgtctc agggcaaccc tggcctgggc agcctgctga acatcaaggc ggaagcggag
     3901 gggagccccg ctgcggagcc ctcgcccttc ctaggcaagg ccgtgaaggc gctggttcag
     3961 gagaagttgg cagagccctg gaaggtgtac ctgcgcaggt ttggtacaaa ggacttctgt
     4021 gacggccagt gtgacttcct ccacaaggcc cacttccact gcgtggtgga ggaatgcggc
     4081 gcgctcttca gcaccttgga cggggccatc aagcacgcaa acttccactt ccggacagag
     4141 ggaggagcag caaaaggaaa cacagaggct gcctttccgg cctcggccgc cgagaccaaa
     4201 cctcccatgg ccccctcgtc ccctccggtc cctcctgtca ccacggccac ggtgtcctct
     4261 ctggaggggc ccgctcccag cccggcctcc gtgccctcca cccccaccct gctcgcctgg
     4321 aagcagctgg cttccaccat accccagatg cctcagatcc cagcgtcagt gcctcacctg
     4381 cccgcctcgc ccttggcaac gacttctcta gagaacgcca agccccaggt caaacccgga
     4441 ttcctccagt tccaggagaa cgatccttgc ctcgccacgg actgcaagta cgccaacaag
     4501 ttccacttcc actgtctctt tgggaactgc aagtacgtct gcaaaacgtc tggcaaggcc
     4561 gaatcccact gcctggacca catcaacccc aacaacaacc tggtgaacgt gcgagaccag
     4621 tttgcatact actctctgca gtgtctctgt cccaaccagc actgcgagtt ccgaatgcgt
     4681 gggcactacc actgcctccg caccggctgc tattttgtga ccaacatcac caccaagctc
     4741 ccctggcaca tcaagaagca tgagaaggcg gagcggcggg cagccaatgg cttcaaatac
     4801 ttcaccaagc gcgaggagtg tggcaggcta ggttgcaagt acaaccaggt gaacagccac
     4861 ttccactgca tccgggaggg ctgccagttc tccttcctcc tcaagcacca gatgacctcc
     4921 cacgcgcgga agcacatgcg gaggatgctg gggaagaact tcgaccgcgt gcccccctcc
     4981 cagggccccc caggcctgat ggatgctgag acagatgagt gcatggacta cactggctgc
     5041 agcccaggcg ccatgtcctc tgagtcatcc accatggacc ggagctgctc cagcaccccc
     5101 gtgggtaacg agagcaccgc ggcagggaac accatctcta tgccgacagc ctcgggggcc
     5161 aaaaagcgct tctggatcat cgaggacatg tcgcccttcg gcaagcggcg gaagacggcg
     5221 tcctcccgga agatgctgga cgagggcatg atgctggagg gcttccggcg cttcgacctt
     5281 tacgaggact gcaaggacgc agcttgtcag ttctcgctca aggtcaccca ctaccactgc
     5341 acgcgcgaga actgcggcta caagttctgc gggcgcacgc acatgtacaa gcacgcgcag
     5401 caccacgacc gcgtggacaa cctggtgctg gacgacttca agcgcttcaa ggcctcactc
     5461 agctgccact tcgccgactg ccccttctcg ggcaccagca cgcacttcca ctgcctgcgc
     5521 tgccgcttcc gctgcaccga cagcaccaag gtcacggcgc atcgcaagca ccacggcaaa
     5581 caggacgtga tcagcgccgc gggcttctgc cagttcagct ccagcgccga ctgcgccgtg
     5641 cccgactgca agtacaagct caagtgctcg cacttccact gcaccttccc gggctgccgc
     5701 cacacggtgg tgggcatgtc gcagatggac tcgcacaagc gcaagcacga gaagcaggag
     5761 cgcggcgagc ccgcggcaga gggccccgcg cccgggcctc ccatcagtct ggacggctcc
     5821 ctgtcgctgg gcgccgagcc gggctcgctg ctcttcctgc agtcggcggc cgccggcctg
     5881 ggcctggcgc tgggcgacgc gggcgacccc ggcccgcccg acgccgccgc ccccgggccg
     5941 cgcgagggcg ccgccgccgc cgccgccgca gctggggagt cctcgcagga ggacgaggag
     6001 gaggagctgg agctgccgga ggaggaggcc gaagacgacg aggacgagga cgacgacgag
     6061 gacgacgacg acgaggacga cgacgaggac gacgacgacg aggacctgcg caccgactcg
     6121 gaggagtcgc tgcccgaggc ggcggcggag gcggcgggcg cgggcgcgcg gaccccggcc
     6181 ttggcggcgc tggcggccct gggcgccccc ggccccgcgc ccactgcagc ctcctcgccc
     6241 ctcgaggact acaaagacca tgacggtgat tataaagatc atgacatcga ctacaaggat
     6301 gacgatgaca agtagtgagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     6361 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6421 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6481 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6541 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6601 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6661 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6721 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6781 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6841 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6901 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6961 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7021 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7081 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7141 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7201 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7261 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7321 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7381 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     7441 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     7501 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7561 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7621 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7681 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7741 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7801 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7861 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7921 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7981 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     8041 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     8101 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     8161 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     8221 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     8281 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     8341 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8401 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8461 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8521 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8581 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8641 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8701 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8761 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8821 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8881 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8941 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9001 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9061 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9121 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9181 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9241 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9301 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9361 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9421 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9481 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     9541 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9601 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9661 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9721 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     9781 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9841 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     9901 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9961 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10021 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10081 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10141 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10201 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10261 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10321 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10381 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10441 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10501 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10561 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10621 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10681 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10741 acctgacgtc
//
