LOCUS V024875 6644 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024875 VERSION V024875 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2134 /label="CADM3(NM_001127173)" /note="CADM3(NM_001127173)" /gene="CADM3" CDS 2141..2206 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2244..2468 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2514..2942 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2956..3285 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3352..4146 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4320..4441 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4490..4506) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4514..4530 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4538..4568) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4583..4604 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4892..5477) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5648..6508) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6509..6613) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgggggccc cagccgcctc 961 gctcctgctc ctgctcctgc tgttcgcctg ctgctgggcg cccggcgggg ccaacctctc 1021 ccaggacgac agccagccct ggacatctga tgaaacagtg gtggctggtg gcaccgtggt 1081 gctcaagtgc caagtgaaag atcacgagga ctcatccctg caatggtcta accctgctca 1141 gcagactctc tactttgggg agaagagagc ccttcgagat aatcgaattc agctggttac 1201 ctctacgccc cacgagctca gcatcagcat cagcaatgtg gccctggcag acgagggcga 1261 gtacacctgc tcaatcttca ctatgcctgt gcgaactgcc aagtccctcg tcactgtgct 1321 aggaattcca cagaagccca tcatcactgg ttataaatct tcattacggg aaaaagacac 1381 agccacccta aactgtcagt cttctgggag caagcctgca gcccggctca cctggagaaa 1441 gggtgaccaa gaactccacg gagaaccaac ccgcatacag gaagatccca atggtaaaac 1501 cttcactgtc agcagctcgg tgacattcca ggttacccgg gaggatgatg gggcgagcat 1561 cgtgtgctct gtgaaccatg aatctctaaa gggagctgac agatccacct ctcaacgcat 1621 tgaagtttta tacacaccaa ctgcgatgat taggccagac cctccccatc ctcgtgaggg 1681 ccagaagctg ttgctacact gtgagggtcg cggcaatcca gtcccccagc agtacctatg 1741 ggagaaggag ggcagtgtgc cacccctgaa gatgacccag gagagtgccc tgatcttccc 1801 tttcctcaac aagagtgaca gtggcaccta cggctgcaca gccaccagca acatgggcag 1861 ctacaaggcc tactacaccc tcaatgttaa tgaccccagt ccggtgccct cctcctccag 1921 cacctaccac gccatcatcg gtgggatcgt ggctttcatt gtcttcctgc tgctcatcat 1981 gctcatcttc cttggccact acttgatccg gcacaaagga acctacctga cacatgaggc 2041 aaaaggctcc gacgatgctc cagacgcgga cacggccatc atcaatgcag aaggcgggca 2101 gtcaggaggg gacgacaaga aggaatattt catcctcgag gactacaaag accatgacgg 2161 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccgt 2221 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 2281 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 2341 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 2401 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 2461 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 2521 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 2581 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 2641 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 2701 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 2761 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 2821 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 2881 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 2941 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 3241 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 3301 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 3361 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 3421 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 3481 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 3541 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 3601 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 3661 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 3721 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 3781 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 3841 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 3901 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 3961 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 4021 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 4081 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 4141 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 4201 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 4261 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 4321 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 4381 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 4441 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 4501 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 4561 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 4621 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 4681 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 4741 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 4801 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 4861 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 4921 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 4981 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5041 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 5101 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 5161 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5221 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5281 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 5341 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5401 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca 5461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 5521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 5581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 5641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 5701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 5761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 5821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 5881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 5941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 6001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 6061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 6121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 6181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 6241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 6301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 6361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 6421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 6481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 6541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 6601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc //