LOCUS V025354 6296 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025354 VERSION V025354 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..1786 /label="C1QBP(NM_001212)" /note="C1QBP(NM_001212)" /gene="C1QBP" CDS 1793..1858 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 1896..2120 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2166..2594 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2608..2937 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3004..3798 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3972..4093 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4142..4158) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4166..4182 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4190..4220) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4235..4256 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4544..5129) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5300..6160) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6161..6265) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgctgcctc tgctgcgctg 961 cgtgccccgt gtgctgggct cctccgtcgc cggcctccgc gctgccgcgc ccgcctcgcc 1021 tttccggcag ctcctgcagc cggcaccccg gctgtgcacc cggcccttcg ggctgctcag 1081 cgtgcgcgca ggttccgagc ggcggccggg cctcctgcgg cctcgcggac cctgcgcctg 1141 tggctgtggc tgcggctcgc tgcacaccga cggagacaaa gcttttgttg atttcctgag 1201 tgatgaaatt aaggaggaaa gaaaaattca gaagcataaa accctcccta agatgtctgg 1261 aggttgggag ctggaactga atgggacaga agcgaaatta gtgcggaaag ttgccgggga 1321 aaaaatcacg gtcactttca acattaacaa cagcatccca ccaacatttg atggtgagga 1381 ggaaccctcg caagggcaga aggttgaaga acaggagcct gaactgacat caactcccaa 1441 tttcgtggtt gaagttataa agaatgatga tggcaagaag gcccttgtgt tggactgtca 1501 ttatccagag gatgaggttg gacaagaaga cgaggctgag agtgacatct tctctatcag 1561 ggaagttagc tttcagtcca ctggcgagtc tgaatggaag gatactaatt atacactcaa 1621 cacagattcc ttggactggg ccttatatga ccacctaatg gatttccttg ccgaccgagg 1681 ggtggacaac acttttgcag atgagctggt ggagctcagc acagccctgg agcaccagga 1741 gtacattact tttcttgaag acctcaagag ttttgtcaag agccagctcg aggactacaa 1801 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta 1861 gtgagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 1921 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 1981 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 2041 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 2101 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 2161 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 2221 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 2281 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 2341 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 2401 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 2461 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 2521 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 2581 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 2641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 2701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2761 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 2821 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 2881 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 2941 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 3001 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 3061 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 3121 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 3181 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 3241 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 3301 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 3361 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 3421 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 3481 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 3541 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 3601 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 3661 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 3721 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 3781 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 3841 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 3901 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 3961 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 4021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 4081 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 4141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 4321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 4381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 4441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 4501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 4981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc 5101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 5161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 5221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 5281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 5581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 5641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 5701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 5821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 5941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 6001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //