LOCUS       pcDNA3.1-App(mouse)     7697 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035878
VERSION     V035878
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3217
                     /label="App(NM_001198823)"
                     /note="App(NM_001198823)"
                     /gene="App"
     polyA_signal    3264..3488
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3534..3962
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3976..4305
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4372..5166
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5340..5461
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5510..5526)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5534..5550
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5558..5588)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5603..5624
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5912..6497)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6668..7528)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7529..7633)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg ctgcccagct tggcactgct cctgctggcc gcctggacgg ttcgggctct
      961 ggaggtaccc actgatggca acgccgggct gctggcagaa ccccagatcg ccatgttctg
     1021 tggtaaactc aacatgcaca tgaatgtgca gaatggaaag tgggagtcag acccgtcagg
     1081 gaccaaaacc tgcattggca ccaaggaggg catcttgcag tactgccaag aggtctaccc
     1141 tgaactgcag atcacaaacg tggtggaagc caaccagcca gtgaccatcc agaactggtg
     1201 caagcggggc cgcaagcagt gcaagacaca cacccacatc gtgattcctt accgttgcct
     1261 agttggtgag tttgtgagcg acgcccttct cgtgcccgac aagtgcaagt tcctacacca
     1321 ggagcggatg gatgtttgtg agacccatct tcactggcac accgtcgcca aagagacatg
     1381 cagcgagaag agcactaact tgcacgacta tggcatgctg ctgccctgcg gcatcgacaa
     1441 gttccgaggg gtagagtttg tatgctgccc gttggccgag gaaagcgaca gcgtggattc
     1501 tgcggatgca gaggaggatg actctgatgt ctggtggggt ggagcggaca cagactacgc
     1561 tgatggcggt gaagacaaag tagtagaagt cgccgaagag gaggaagtgg ctgatgttga
     1621 ggaagaggaa gctgatgatg atgaggatgt ggaggatggg gacgaggtgg aggaggaggc
     1681 cgaggagccc tacgaagagg ccaccgagag aacaaccagc actgccacca ccaccacaac
     1741 caccactgag tccgtggagg aggtggtccg agaggtgtgc tctgaacaag ccgagaccgg
     1801 gccatgccgc gcaatgatct cccgctggta ctttgatgtc actgaaggga agtgtgtccc
     1861 attcttttac ggcggatgtg gcggcaacag gaacaacttt gacacggaag agtactgcat
     1921 ggcggtgtgt ggcagcgtgt caacccaaag tttactcaag actaccagtg aacctcttcc
     1981 ccaagatcct gataaacttc ccacgacagc agccagcacc cccgacgccg tcgacaagta
     2041 cctggagaca cccggggacg agaacgagca tgcccatttc cagaaagcca aagagaggct
     2101 ggaagccaag caccgagaga gaatgtccca ggtcatgaga gaatgggaag aggcagagcg
     2161 tcaagccaag aacttgccca aagctgacaa gaaggccgtt atccagcatt tccaggagaa
     2221 agtggaatct ctggaacagg aagcagccaa tgagagacag cagcttgtag agacacacat
     2281 ggccagagtt gaagccatgc tcaatgaccg ccgccgcctg gccctcgaga attacatcac
     2341 tgcactgcag gcggtgcccc caaggcctca tcatgtgttc aacatgctga agaagtacgt
     2401 ccgtgcggag cagaaagaca gacagcacac cctaaagcat tttgaacatg tgcgcatggt
     2461 ggaccccaag aaagctgctc agatccggtc ccaggttatg acacacctcc gtgtgatcta
     2521 cgagcgcatg aaccagtctc tgtccctgct ctacaatgtc cctgcggtgg ctgaggagat
     2581 tcaagatgaa gtcgatgagc tgcttcagaa ggagcagaac tactccgacg atgtcttggc
     2641 caacatgatc agtgagccca gaatcagcta cggaaacgac gctctcatgc cttcgctgac
     2701 ggaaaccaag accaccgtgg agctccttcc cgtgaatggg gaattcagcc tggatgacct
     2761 ccagccgtgg cacccttttg gggtggactc tgtgccagcc aataccgaaa atgaagtcga
     2821 gcctgttgac gcccgccccg ctgctgaccg aggactgacc actcgaccag gttctgggct
     2881 gacaaacatc aagacggaag agatctcgga agtgaagatg gatgcagaat tcggacatga
     2941 ttcaggattt gaagtccgcc atcaaaaact ggtgttcttt gctgaagatg tgggttcgaa
     3001 caaaggcgcc atcatcggac tcatggtggg cggcgttgtc atagcaaccg tgattgtcat
     3061 caccctggtg atgttgaaga agaaacagta cacatccatc catcatggcg tggtggaggt
     3121 cgacgccgcc gtgaccccag aggagcgcca tctctccaag atgcagcaga acggatatga
     3181 gaatccaact tacaagttct ttgagcaaat gcagaactaa ctcgagtcta gagggcccgt
     3241 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3301 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3361 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3421 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     3481 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     3541 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3601 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3661 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3721 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3781 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3841 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3901 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3961 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4021 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4081 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4141 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4201 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4261 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4321 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4381 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4441 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4501 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     4561 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     4621 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4681 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4741 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4801 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4861 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     4921 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4981 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5041 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5101 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5161 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5221 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5281 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5341 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5401 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5461 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5521 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5581 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5641 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5701 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5761 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5821 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5881 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5941 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6001 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6061 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6121 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6181 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6241 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6301 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6361 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6421 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     6481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6661 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6721 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6781 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6841 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6901 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6961 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7021 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7081 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7141 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7201 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7261 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7321 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7381 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7441 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7501 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7561 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7621 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7681 tcccgatccc ctatggt
//
