LOCUS       V026760                 7801 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026760
VERSION     V026760
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3240
                     /label="App(NM_001198823)"
                     /note="App(NM_001198823)"
                     /gene="App"
     CDS             3247..3273
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3277..3303
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3307..3333
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3368..3592
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3638..4066
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4080..4409
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4476..5270
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5444..5565
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5614..5630)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5638..5654
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5662..5692)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5707..5728
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6016..6601)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6772..7632)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7633..7737)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgctgccca gcttggcact gctcctgctg
      961 gccgcctgga cggttcgggc tctggaggta cccactgatg gcaacgccgg gctgctggca
     1021 gaaccccaga tcgccatgtt ctgtggtaaa ctcaacatgc acatgaatgt gcagaatgga
     1081 aagtgggagt cagacccgtc agggaccaaa acctgcattg gcaccaagga gggcatcttg
     1141 cagtactgcc aagaggtcta ccctgaactg cagatcacaa acgtggtgga agccaaccag
     1201 ccagtgacca tccagaactg gtgcaagcgg ggccgcaagc agtgcaagac acacacccac
     1261 atcgtgattc cttaccgttg cctagttggt gagtttgtga gcgacgccct tctcgtgccc
     1321 gacaagtgca agttcctaca ccaggagcgg atggatgttt gtgagaccca tcttcactgg
     1381 cacaccgtcg ccaaagagac atgcagcgag aagagcacta acttgcacga ctatggcatg
     1441 ctgctgccct gcggcatcga caagttccga ggggtagagt ttgtatgctg cccgttggcc
     1501 gaggaaagcg acagcgtgga ttctgcggat gcagaggagg atgactctga tgtctggtgg
     1561 ggtggagcgg acacagacta cgctgatggc ggtgaagaca aagtagtaga agtcgccgaa
     1621 gaggaggaag tggctgatgt tgaggaagag gaagctgatg atgatgagga tgtggaggat
     1681 ggggacgagg tggaggagga ggccgaggag ccctacgaag aggccaccga gagaacaacc
     1741 agcactgcca ccaccaccac aaccaccact gagtccgtgg aggaggtggt ccgagaggtg
     1801 tgctctgaac aagccgagac cgggccatgc cgcgcaatga tctcccgctg gtactttgat
     1861 gtcactgaag ggaagtgtgt cccattcttt tacggcggat gtggcggcaa caggaacaac
     1921 tttgacacgg aagagtactg catggcggtg tgtggcagcg tgtcaaccca aagtttactc
     1981 aagactacca gtgaacctct tccccaagat cctgataaac ttcccacgac agcagccagc
     2041 acccccgacg ccgtcgacaa gtacctggag acacccgggg acgagaacga gcatgcccat
     2101 ttccagaaag ccaaagagag gctggaagcc aagcaccgag agagaatgtc ccaggtcatg
     2161 agagaatggg aagaggcaga gcgtcaagcc aagaacttgc ccaaagctga caagaaggcc
     2221 gttatccagc atttccagga gaaagtggaa tctctggaac aggaagcagc caatgagaga
     2281 cagcagcttg tagagacaca catggccaga gttgaagcca tgctcaatga ccgccgccgc
     2341 ctggccctcg agaattacat cactgcactg caggcggtgc ccccaaggcc tcatcatgtg
     2401 ttcaacatgc tgaagaagta cgtccgtgcg gagcagaaag acagacagca caccctaaag
     2461 cattttgaac atgtgcgcat ggtggacccc aagaaagctg ctcagatccg gtcccaggtt
     2521 atgacacacc tccgtgtgat ctacgagcgc atgaaccagt ctctgtccct gctctacaat
     2581 gtccctgcgg tggctgagga gattcaagat gaagtcgatg agctgcttca gaaggagcag
     2641 aactactccg acgatgtctt ggccaacatg atcagtgagc ccagaatcag ctacggaaac
     2701 gacgctctca tgccttcgct gacggaaacc aagaccaccg tggagctcct tcccgtgaat
     2761 ggggaattca gcctggatga cctccagccg tggcaccctt ttggggtgga ctctgtgcca
     2821 gccaataccg aaaatgaagt cgagcctgtt gacgcccgcc ccgctgctga ccgaggactg
     2881 accactcgac caggttctgg gctgacaaac atcaagacgg aagagatctc ggaagtgaag
     2941 atggatgcag aattcggaca tgattcagga tttgaagtcc gccatcaaaa actggtgttc
     3001 tttgctgaag atgtgggttc gaacaaaggc gccatcatcg gactcatggt gggcggcgtt
     3061 gtcatagcaa ccgtgattgt catcaccctg gtgatgttga agaagaaaca gtacacatcc
     3121 atccatcatg gcgtggtgga ggtcgacgcc gccgtgaccc cagaggagcg ccatctctcc
     3181 aagatgcagc agaacggata tgagaatcca acttacaagt tctttgagca aatgcagaac
     3241 ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     3301 gctagctatc cttatgatgt cccagactac gcatgagggc ccgtttaaac ccgctgatca
     3361 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3421 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3481 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3541 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3601 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3901 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4081 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4141 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4201 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4261 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4321 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4381 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4441 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4501 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4561 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4621 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     4681 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4741 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4801 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4861 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4921 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4981 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     5041 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5101 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5161 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5221 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     5281 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     5341 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     5401 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     5461 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5521 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     5581 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5641 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5701 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5761 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5821 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5881 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5941 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6001 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6061 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6541 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6601 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6661 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6721 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6781 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6841 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6901 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6961 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7021 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7081 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7141 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7201 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7261 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7321 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7381 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7441 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7501 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7561 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7621 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7681 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7741 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7801 t
//
