LOCUS V032238 9456 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V032238 VERSION V032238 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 919..4269 /label="Adamts6(NM_001081020)" /note="Adamts6(NM_001081020)" /gene="Adamts6" CDS 4306..5025 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 5056..5280 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 5326..5754 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5768..6097 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6164..6958 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7132..7253 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7302..7318) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7326..7342 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7350..7380) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7395..7416 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7704..8289) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8460..9320) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9321..9425) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggaaatttcg tggaaaacgt tgacttggat tctgagcctc 961 atcatggcct catcggaatt ttatagtgac cacagacttt catatagttc tcaagaggaa 1021 ttcctgactt atcttgaaca ctaccagcta actattccaa taagagttga ccaaaatgga 1081 gcttttctca gctttactgt gaaaaatgat aaacactcaa gaagaagacg gagtatggac 1141 cttcttgatc cacagcaggc agtgtctaaa ttatttttta aactttcagc ctatggcaag 1201 cactttcacc taaacttgac tctcaataca aattttgtgt ccaaacattt tacagtagag 1261 tattggggga aagatggacc ccagtggaaa catgattttt tagacaactg tcactacaca 1321 ggatacttgc aagatcaaca tagtacaact aaagtggctt taagcaactg tgttggattg 1381 catggtatta ttgctacaga agatgaagaa tattttattg agcctctaaa gaatactaca 1441 gaggattcca aacattttag ttatgaaaat ggccatcctc atgttattta caaaaagtct 1501 actcttcagc aacaacatat ctatgatcat tctcattgtg gggtttcaga tctcacagga 1561 agtggcaaac cttggtggct gaataagaca tcatcttttc cttctttacc accaattaat 1621 gacacacata ttcatcacag aaagagaaga tctgtgagca ctgagagatt tgtggagact 1681 ttagtagtgg cagacaaaat gatggtgggc taccatggcc gtaaagacat tgaacattat 1741 attttaagtg tgatgaatat tgttgccaaa ctttatcgtg actccagtct aggaaacgtt 1801 gtgaatatta tagtggctcg cttaattgtt ctcactgaag atcagccaaa cttggagata 1861 aaccatcatg cagacaagtc cctcgatagc ttctgtaaat ggcagaaatc cattctctcc 1921 caccaaagtg atggaaacac cattccagaa aatgggattg cccaccacga taatgcagtt 1981 cttattacta gatatgatat ctgcacgtat aaaaacaagc cctgtggaac actgggctta 2041 gcctctgtgg ctggaatgtg tgagcctgaa aggagctgca gcattaatga agacattggc 2101 ctaggctcgg cttttaccat cgcacatgag attggtcaca attttggtat gaaccatgat 2161 ggaattggaa attcttgtgg gacgaaaggt catgaggcag caaaacttat ggcagctcat 2221 attactgcaa ataccaatcc cttctcctgg tctgcctgca gcagagacta catcaccagc 2281 tttctagatt caggccgtgg tacttgcctt gataatgagc ctccaaagcg tgactttctt 2341 tatccagctg tggccccagg tcaggtgtat gatgctgatg aacaatgtcg tttccagtat 2401 ggagcaacat cccgccaatg caaatatggg gaagtgtgta gagagctctg gtgcctcagc 2461 aaaagcaacc gctgtgtcac caacagtatt ccagctgctg aggggacact gtgtcagact 2521 gggaatattg agaaagggtg gtgttatcag ggagattgtg ttccttttgg cacatggccc 2581 cagagtattg atgggggctg gggttcctgg tcactgtggg gcgagtgcag taggacctgc 2641 ggaggaggtg tctcctcgtc tctaagacac tgtgacagtc cagcaccttc aggaggtggg 2701 aaatattgcc ttggtgaaag gaaacgctac cgctcttgca acacagatcc atgccctttg 2761 ggttcccgag attttcgaga aaaacagtgt gcagactttg acagtatgcc tttccgggga 2821 aagcattata actggaaacc ctatactgga ggtggggtaa aaccttgtgc attaaattgc 2881 ctggctgaag gctataattt ctacacagag cgagctcctg ctgtgataga tgggacacag 2941 tgcaatgcag actcactgga tatctgtatc aatggagaat gcaagcacgt aggttgtgat 3001 aatattttgg gatctgatgc tagggaagat aggtgccgag tctgtggtgg ggatggaagc 3061 acctgtgatg ccattgaagg gttcttcaat gactcattgc ccagaggagg ttatatggaa 3121 gtggttcaga taccaagagg ctctgttcat attgaagtaa gagaagtttc catgtcaaaa 3181 aattacattg ctttaaaatc tgaaggggat gattactaca ttaatggtgc ctggactatt 3241 gactggccta ggaaatttga tgtttcgggg acagcttttc actacaagag accgacagat 3301 gaaccagaat ccttggaagc tctaggaccc acctctgaga acctcattgt aatggttcta 3361 ctccaagaac agaatttggg aattaggtac aagttcaatg ttcccatcgt tcgtactggc 3421 agtggagata atgaagttgg cttcatgtgg acttatcagc cctgggcgga gtgctctgct 3481 acttgtgctg gaggtgtcca aagacaagaa gtggtctgta aaagattgga tgacaactcc 3541 attgtccaga acaatttctg tgatcctgac agtaagccac ctgaaaatca aagagcctgc 3601 aacactgagc cttgcccacc tgaatggttc attggagact ggttggagtg cagcaagact 3661 tgtgatggtg ggatgcgcac aagggccgtg ctctgcatca ggaaagttgg accttctgaa 3721 gaggaaacac tggactacgg cgactgctta acacaccggc ctgtggagaa agagtcctgc 3781 aacaaccagt catgtccacc tcagtgggta gctttggact ggtccgaatg tactcctaaa 3841 tgtggttcag gattcaagca tcggattgtt ctgtgcaaga gcagtgacct ttctaaaaca 3901 ttcccagctg cacaatgtcc agaggagagc aagcctcctg ctcgcatccg ctgcagtctg 3961 ggccgctgcc ctcctccccg ctgggtgaca ggagactggg gccagtgctc tgctcagtgt 4021 ggtcttggac aacaaatgag aactgtccag tgcctttcct atactggaca ggcgtctgtt 4081 gactgtccag aaactgtccg gcctccatcc atgcagcagt gtgacagcaa atgtgatagc 4141 acccctctct ccagtactga agagtgcaaa gatgtgaaca aagtggctta ttgcccactg 4201 gtgctgaagt tcaagttctg cagtcgagca tacttcagac agatgtgttg taagacctgc 4261 caaggacact cgagtggcgg tggaggaagc ggcggaggtg gatctatggt gagcaagggc 4321 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 4381 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 4441 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 4501 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 4561 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 4621 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 4681 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 4741 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 4801 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 4861 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 4921 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 4981 accgccgccg ggatcactct cggcatggac gagctgtaca agtagggccc gtttaaaccc 5041 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 5101 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 5161 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 5221 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 5281 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 5341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 5401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 5461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 5521 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 5581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 5641 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 5701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 5761 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 5821 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 5881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 5941 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 6001 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 6061 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 6121 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 6181 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 6241 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 6301 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 6361 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 6421 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 6481 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 6541 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 6601 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 6661 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 6721 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 6781 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 6841 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 6901 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 6961 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 7021 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 7081 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 7141 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 7201 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 7261 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 7321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 7381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 7441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 7501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 7561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 7621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 7681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 7741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 7801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 7861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 7921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 7981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 8041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 8101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 8161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 8221 caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 8281 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 8341 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 8401 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 8461 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 8521 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 8581 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 8641 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 8701 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 8761 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 8821 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 8881 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 8941 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 9001 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 9061 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 9121 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 9181 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 9241 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 9301 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 9361 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 9421 ccgcgcacat ttccccgaaa agtgccacct gacgtc //