LOCUS       V047561                 7588 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047561
VERSION     V047561
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3027
                     /label="Acsl1(NM_007981)"
                     /note="Acsl1(NM_007981)"
                     /gene="Acsl1"
     CDS             3034..3060
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3064..3090
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3094..3120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3155..3379
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3425..3853
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3867..4196
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4263..5057
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5231..5352
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5401..5417)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5425..5441
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5449..5479)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5494..5515
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5803..6388)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6559..7419)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7420..7524)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggaagtcc atgaattgtt ccggtatttt
      961 cgaatgccag agctgattga cattcggcag tacgtgcgca cccttccaac caacaccctc
     1021 atggggtttg gggcttttgc agcgctcacc accttctggt atgccaccag gcctaaggcc
     1081 ctgaagccac catgtgacct ctccatgcag tcagtggaaa tagcgggtac cactgatggt
     1141 attcgaagat cagcagtcct tgaagatgac aagctcttgg tgtactacta cgacgatgtc
     1201 agaaccatgt acgatggctt ccagaggggg attcaggtgt caaataatgg tccttgttta
     1261 ggttctcgga agccaaacca gccctatgag tggatttcct acaaagaggt ggcagaactg
     1321 gctgagtgca taggctccgg gctgatccag aaggggttca agccttgctc cgagcagttc
     1381 atcggcctct tctctcaaaa cagacccgag tgggtgatcg tcgagcaagg atgcttctct
     1441 tactcaatgg tggtcgtccc gctctatgac acccttggag ctgacgccat cacctacata
     1501 gtgaacaaag ctgaactctc tgtgattttt gctgacaagc cagaaaaagc caaactctta
     1561 ttagaaggtg tagaaaacaa gttaacacca tgccttaaaa tcatagtcat catggactcc
     1621 tacggcagtg atctggtgga acgaggcaag aagtgtgggg tggaaatcat cagcctcaaa
     1681 gctctggagg accttggaag agtgaacaga gtgaagccca agcctccaga acccgaagat
     1741 cttgcgataa tttgtttcac aagtggaact acaggcaacc ccaaaggagc aatgatcact
     1801 caccaaaaca ttataaacga ctgctcaggt tttataaaag caacagagag tgcattcatc
     1861 gcttccacag atgatgtgct gatatctttc ttgcctctcg cccatatgtt tgagaccgtt
     1921 gtagagtgtg taatgctgtg tcatggagct aagataggat ttttccaagg agatatcagg
     1981 ctgcttatgg acgacctcaa ggtgcttcag cccaccatct tccctgtggt tcccaggctg
     2041 ctgaaccgga tgttcgacag aatttttgga caagcaaaca cttccttgaa gcgatggctg
     2101 ttggactttg cctccaaaag gaaagaggcg gagcttcgca gtggcatcgt cagaaacaac
     2161 agcctgtggg ataaactcat cttccacaag atacagtcga gcctgggtgg gaaagtccgg
     2221 ctgatgatca caggagcagc cccggtgtct gccacagtgc tgacgtttct gaggacagcg
     2281 ctcggctgcc agttctatga aggctacgga cagaccgagt gcactgctgg ttgctgcctg
     2341 agcttgcccg gagactggac ggcaggccat gttggagccc ccatgccttg caattatgta
     2401 aagcttgtgg atgtggaaga aatgaattac ctggcatcca agggcgaggg tgaggtgtgt
     2461 gtgaaagggg caaatgtgtt caaaggctac ttgaaagacc cagcaagaac agctgaagcc
     2521 ctggataaag atggctggtt acacacgggg gacattggaa aatggctgcc aaatggcacc
     2581 ttgaagatta tcgacaggaa aaagcacata tttaaactag cccaaggaga gtacatagca
     2641 ccagaaaaga ttgaaaatat ctacctgcgg agtgaagccg tggcccaggt gtttgtccac
     2701 ggagaaagct tgcaggcctt tctcatagca gttgtggtac ccgacgttga gagcctaccg
     2761 tcctgggcac agaagagagg cttacaaggg tccttcgaag aactgtgcag gaacaaggat
     2821 atcaataaag ctatcctgga cgacttgttg aaacttggga aggaagccgg tctgaagcca
     2881 tttgaacagg tcaaaggcat tgctgtgcac ccggaattat tttctattga caacggcctt
     2941 ctgactccaa cactgaaggc gaagaggcca gagctacgga actatttcag gtcgcagata
     3001 gatgaactgt acgccaccat caagatcctc gagtacccat acgacgtacc tgattatgca
     3061 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     3121 tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3361 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3421 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3841 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3901 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4021 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4201 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     4261 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4441 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4741 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     4801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4921 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5041 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5101 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5161 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5221 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5341 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5401 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5461 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5521 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5581 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     6361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     7561 atcgggagat ctcccgatcc cctatggt
//
