LOCUS       V018496                 8803 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018496
VERSION     V018496
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4233
                     /label="Acly(NM_001199296)"
                     /note="Acly(NM_001199296)"
                     /gene="Acly"
     CDS             4249..4275
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4279..4305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4309..4335
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4370..4594
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4640..5068
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5082..5411
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5478..6272
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6446..6567
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6616..6632)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6640..6656
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6664..6694)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6709..6730
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7018..7603)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7774..8634)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8635..8739)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgtcagcca aggcaatttc agagcagacc
      961 ggcaaagaac tcctgtacaa gtacatctgc accacctcgg ccatccagaa ccggttcaag
     1021 tacgcccggg tgactcccga cacagactgg gcccatctgc tgcaggacca cccgtggctg
     1081 ctcagccaga gcttggttgt caagccagac cagttaatca aacgtcgagg aaagcttggc
     1141 ctcgtcgggg tcaatctctc tctggatgga gtcaaatcct ggctaaaacc tcgcctggga
     1201 catgaagcca ctgtcggcaa ggccaaaggc ttcctcaaga actttctcat tgaacccttc
     1261 gtcccccaca gtcaggcgga ggagttctac gtgtgcatct atgctacccg ggaaggagac
     1321 tacgtcctgt tccaccatga agggggtgtg gatgtgggcg atgtggatgc caaagcccag
     1381 aagctgcttg tgggtgtgga cgaaaagctg aataccgagg acattaagag acacctgttg
     1441 gtccatgcac ctgaggacaa gaaagaagtc ctggccagct tcatctctgg tctattcaat
     1501 ttctacgagg atctgtactt cacctacctt gagatcaacc cccttgtggt gaccaaagat
     1561 ggtgtctaca tccttgactt ggcggccaag gtggatgcca cagctgacta catctgtaaa
     1621 gtcaagtggg gtgatataga gttccctccc ccctttgggc gtgaggcgta ccccgaggaa
     1681 gcctacattg cagacctgga tgccaaaagt ggagcaagct tgaagctgac cttgctgaac
     1741 cccaaggggc ggatctggac catggttgct gggggtggcg cctccgtcgt gtacagtgac
     1801 accatctgtg atcttggagg tgtcaatgaa ctggcgaatt acggggaata ctcgggtgcc
     1861 cccagtgaac aacagaccta tgactatgcc aagaccatcc tctcacttat gactcgagag
     1921 aagcacccag aaggcaagat cctcatcatt ggaggcagca ttgcaaactt caccaatgtg
     1981 gccgccacct tcaagggcat tgtgagagcg attcgagatt accagggtcc cctgaaggag
     2041 catgaggtca ccatctttgt ccgaagaggt ggccccaact atcaagaggg attacgagtg
     2101 atgggagaag ttgggaagac cactgggatc cccatccatg tctttggcac agaaactcac
     2161 atgacggcca ttgtgggcat ggccctgggc caccggccca ttcccaacca gccacccaca
     2221 gcagctcaca ctgccaactt cctccttaat gccagcggga gcacatcgac tccagcaccc
     2281 agtaggacag catctttttc tgagtcccga gctgatgaag tggcacctgc aaagaaagcc
     2341 aagccagcta tgccccaaga ttcagtccca agtccaagat ccctgcaagg aaagagtgcc
     2401 accctcttca gccgacatac caaggccatc gtgtggggca tgcagacccg ggctgtgcag
     2461 ggcatgctgg actttgacta cgtgtgttcc cgagacgagc cctcagtggc tgccatggtc
     2521 taccctttca ctggggatca caagcagaag ttttactggg gacacaagga aatcctgatc
     2581 cctgtcttca agaacatggc tgacgccatg aagaagcacc cggaggtaga cgtgctgatc
     2641 aactttgcgt ctctgcggtc cgcttacgac agcaccatgg agaccatgaa ctatgcccag
     2701 atccgcacca tagccatcat agcagaaggt atccctgagg ccctcacacg gaagctcatc
     2761 aagaaggccg accagaaggg agtgaccatc attgggccag ctacggttgg gggcattaag
     2821 cctggatgct ttaagatcgg gaatactggt ggaatgctgg acaacatcct ggcctccaaa
     2881 ctgtaccgcc caggcagcgt ggcctacgtc tcacgttcag gaggcatgtc taatgaactc
     2941 aataacatca tctctcggac cacagatggt gtctatgagg gcgtggccat cggcggggac
     3001 aggtaccctg ggtccacatt catggatcac gtgctgcgct accaggacac tccaggagtc
     3061 aagatgatcg tagttcttgg ggagataggg ggcacagagg aatataagat ctgccggggc
     3121 atcaaggagg gccgcctcac caagccagtg gtctgctggt gtatcgggac ctgtgccacc
     3181 atgttctcct ccgaggtcca gtttggccat gctggagctt gtgccaacca ggcttctgaa
     3241 actgcagtag ccaagaacca ggccttgaag gaagcaggag tgtttgtgcc ccgaagcttc
     3301 gatgagcttg gagaaatcat tcagtctgtg tatgaagatc tggtggccaa aggagccatt
     3361 gtacctgccc aggaagtgcc acctccaaca gtgcccatgg actactcttg ggccagagag
     3421 ctgggtttga tccgaaaacc tgcctcattc atgaccagca tctgtgatga gcgagggcag
     3481 gagctcattt atgcgggcat gcccatcacc gaggtcttca aggaggagat gggcatcggt
     3541 ggtgtcctcg gcctcctctg gttccagaga aggttgccca agtattcctg ccagttcatt
     3601 gagatgtgtc tgatggtcac agctgatcac gggccagctg tctctggagc ccataacacc
     3661 atcatctgtg ctcgggctgg gaaggacctg gtctccagcc tcacctcagg gctgctcacc
     3721 attggagacc ggtttggggg tgccttggat gccgcagcaa agatgttcag taaagccttt
     3781 gacagcggca tcattcccat ggagttcgtc aacaagatga agaaggaggg gaagctgatc
     3841 atgggcatcg gccatcgagt aaaatcgata aacaacccag acatgcgagt gcagatcctc
     3901 aaggacttcg tcaaacagca cttccccgcc accccgctgc tcgactatgc cctggaagtg
     3961 gagaagatta ccacctccaa gaagccaaat cttatcctga atgtggacgg cttcatcggc
     4021 gttgcgtttg tggacatgct caggaactgt ggctccttca cccgggagga agctgatgaa
     4081 tatgttgaca ttggagccct caatggcatc tttgtgctag gaaggagtat gggcttcatt
     4141 gggcactacc ttgaccagaa gaggctgaag caagggctgt atcgtcaccc ctgggatgac
     4201 atttcctatg ttcttccaga acacatgagc atgcagcggc cgctcgagta cccatacgac
     4261 gtacctgatt atgcaactta tccttatgat gtacctgatt atgctagcta tccttatgat
     4321 gtcccagact acgcatgagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     4381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4561 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4621 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4981 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5041 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5101 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5161 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5221 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5281 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5341 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5401 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     5461 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     5521 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5581 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5641 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5701 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5761 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5821 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5881 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5941 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6001 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6061 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6121 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6181 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6241 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     6301 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     6361 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     6421 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     6481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6541 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     6601 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6661 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6721 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6781 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     7561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7741 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7801 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7861 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7921 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7981 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8041 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8101 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8161 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8221 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8281 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8341 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8401 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8461 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8521 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8581 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8641 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8701 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8761 acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
