LOCUS       V037887                 8343 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037887
VERSION     V037887
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..3858
                     /label="Aars1(NM_146217)"
                     /note="Aars1(NM_146217)"
                     /gene="Aars1"
     CDS             3880..3909
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3938..4162
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4208..4636
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4650..4979
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5046..5840
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6016..6137
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6186..6202)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6210..6226
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6234..6264)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6279..6300
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6588..7176)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7347..8207)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8208..8312)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggat
      961 gccactttaa cagcacgaga gatccgagag cggtttataa atttcttcag acgaaatgag
     1021 cacacctatg tccactcttc cgccaccatc cctctggatg accccactct gctcttcgcc
     1081 aacgctggca tgaaccagtt caaacccatc ttcctaaaca caatcgaccc atctcaccct
     1141 atggcgaagc tgagcagagc tgccaatacc cagaaatgca tccgagctgg gggcaagcac
     1201 aacgacctgg atgacgtggg caaggatgtc taccaccaca ctttctttga gatgctgggc
     1261 tcctggtctt ttggggacta tttcaaggaa ttggcatgta agatggccct ggaacttctt
     1321 acccaggagt ttggtattcc agttgaaaga ctctatgtca cttactttgg tggtgatgag
     1381 gcggccggcc tagagccaga tctggagtgc agacagattt ggcaaaattt aggactggat
     1441 gaagccagaa tcctccctgg caacatgaag gacaacttct gggagatggg tgacacaggc
     1501 ccctgtggtc cctgcagcga gatccactat gaccgcattg gtggcaggga cgccgcacac
     1561 cttgtcaacc aggatgaccc caatgtgctg gagatctgga acctcgtgtt catccagtat
     1621 aacagggagt cggatggtgt tctgaaacct cttcccaaga aaagcatcga cacagggatg
     1681 ggcctagaga ggctggtgtc tgtgctgcag aacaagatgt ccaactacga caccgacctt
     1741 ttcatgcctt acttcgaagc cattcagaag ggcaccggcg cccggccgta tactgggaag
     1801 gttggtgctg aggatgcaga cggaatcgac atggcctacc gggttctggc tgaccacgcc
     1861 cggaccatca ctgtggcgct ggctgatggc ggccggcctg acaacacagg acgggggtac
     1921 gtgctgagac ggatccttcg ccgagctgtt cggtattccc atgagaaact gaatgccagc
     1981 aggggtttct tcgctacatt ggttgatgtt gtcgttcaat ccctgggaga cgcatttcct
     2041 gagctgaaga aggacccaga gatggtgaag gacatcatta atgaagaaga ggtgcagttt
     2101 ctcaagactc tcagccgagg gcggcgcatc ctggaccgaa aaattcagag cttaggagac
     2161 tgcaaaacca tcccaggtga caccgcttgg ctcctctatg atacttatgg attcccagtg
     2221 gacctcactg gattgattgc tgaagagaag ggcctggtgg tagatatgaa tggctttgag
     2281 gaagagagga gactggccca gctgaagtca cagggcaagg gagcagggga cgaagacctc
     2341 atcatgctgg acatttacgc tattgaagag ctccgggcaa agggtttgga ggccacagat
     2401 gattctccaa agtataacta ccaatcagac tccagtggta gctacgtgtt tgagtgtaca
     2461 gtggctaccg tgcttgctct gcgtcgggaa aagatgtttg tggacgaggt ggtcactggc
     2521 caggagtgtg gagtggtgct ggacaagacc tgtttctatg cggagcaagg agggcagatc
     2581 tacgatgaag gctacttggt gaaggtcgac gacagcagtg aggacaaaac cgagtttaca
     2641 gtgaagaacg ctcaggtccg aggcgggtac gtgctgcaca taggaacgat atatggtaac
     2701 ctgaaagtgg gggatcaagt ccggctgttt attgatgagc cccggcggag acccgtcatg
     2761 agcaaccaca cagctacaca catcctcaac ttcgccttgc gctcggtgct tggggaagcg
     2821 gaccagaaag gttcactggt tgctcctgac cgccttcggt ttgacttcac tgccaaggga
     2881 gccatgtcca cccagcaaat caagaaggct gaagagattg tcaatggcat gattgaggca
     2941 gctaagcctg tctacaccca ggattgtccc ctggcagcag caaaagccat ccagggcctt
     3001 cgagctgtgt ttgatgagac ctatcctgac cccgttcgag tcgtctccat tggggtcccc
     3061 gtgtcagagc tgctggatga cccgtgtggg cctgctggct cccttacttc tgttgagttc
     3121 tgtggaggaa cacatctacg gaattctagc cacgcaggcg cttttgtgat agtgacagaa
     3181 gaagctattg ccaagggtat ccggaggatt gttgctgtca caggtgctga agcccagaag
     3241 gctctcagga agtcagaaac cttgaagaaa tctctctctg ccatggaggc caaagtgaag
     3301 gcccagactg cacccaacaa ggacgtgcag agggaaattg ctgacctcgg tgaggccctg
     3361 gccactgcag tcatccccca gtggcagaaa gatgaacaac gagagactct gaagtccctg
     3421 aagaaagtca tggatgacct agaccgggcc agcaaagccg atgtgcagaa gcgagtgtta
     3481 gagaagacaa agcagctgat tgacagcaac cccaaccagc cgctggtcat cctggagatg
     3541 gagagtggag catcggccaa ggccctgaat gaagcgttga agcttttcaa gacgcattct
     3601 cctcagacat ctgccatgct gttcacggtg gacaatgagg ctggcaagat cacgtgcttg
     3661 tgccaggttc cccagaatgc agccaaccgg ggactgaaag ccagcgagtg ggtccagcag
     3721 gtttcaggcc tgatggatgg taaaggcggt ggcaaggaca tgtccgccca ggccacaggc
     3781 aagaacgtgg gctgccttca ggaagcactg cagctggcta cctcctttgc ccagctccgt
     3841 ctgggagatg tgaagaacct cgagtctaga gggcccttcg aacaaaaact catctcagaa
     3901 gaggatctgt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3961 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4021 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4081 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4141 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4201 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4261 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4321 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4381 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4441 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4501 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4561 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4621 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4741 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4801 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4861 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     4921 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4981 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     5041 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5101 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5161 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5221 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5281 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5341 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5401 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5461 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5521 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     5581 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5641 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5701 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5761 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5821 ttcttgacga gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg
     5881 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5941 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     6001 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6121 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6181 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6241 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6301 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6361 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6421 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6481 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6541 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6601 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6661 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6721 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6781 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6841 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6901 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6961 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7021 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7081 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7141 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7201 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7261 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8341 gtc
//
