LOCUS       V028170                 8893 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028170
VERSION     V028170
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4332
                     /label="ATP11A(NM_015205)"
                     /note="ATP11A(NM_015205)"
                     /gene="ATP11A"
     CDS             4339..4365
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4369..4395
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4399..4425
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4460..4684
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4730..5158
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5172..5501
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5568..6362
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6536..6657
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6706..6722)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6730..6746
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6754..6784)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6799..6820
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7108..7693)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7864..8724)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8725..8829)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggactgca gcctcgtgcg gacgctcgtg
      961 cacagatact gtgcaggaga agagaattgg gtggacagca ggaccatcta cgtgggacac
     1021 agggagccac ctccgggcgc agaggcctac atcccacaga gatacccaga caacaggatc
     1081 gtctcgtcca agtacacatt ttggaacttt atacccaaga atttatttga acaattcaga
     1141 agagtagcca acttttattt ccttatcata tttctggtgc agttgattat tgatacaccc
     1201 acaagtccag tgacaagcgg acttccactc ttctttgtca ttactgtgac ggctatcaaa
     1261 cagggttatg aagactggct tcgacataaa gcagacaatg ccatgaacca gtgtcctgtt
     1321 catttcattc agcacggcaa gctcgttcgg aaacaaagtc gaaagctgcg agttggggac
     1381 attgtcatgg ttaaggagga cgagaccttt ccctgcgact tgatcttcct ttccagcaac
     1441 cggggagatg ggacgtgcca cgtcaccacc gccagcttgg atggagaatc cagccataaa
     1501 acgcattacg cggtccagga caccaaaggc ttccacacag aggaggatat cggcggactt
     1561 cacgccacca tcgagtgtga gcagccccag cccgacctct acaagttcgt gggtcgcatc
     1621 aacgtttaca gtgacctgaa tgaccccgtg gtgaggccct taggatcgga aaacctgctg
     1681 cttagaggag ctacactgaa gaacactgag aaaatctttg gtgtggctat ttacacggga
     1741 atggaaacca agatggcatt aaattatcaa tcaaaatctc agaagcgatc tgccgtggaa
     1801 aaatcgatga atgcgttcct cattgtgtat ctctgcattc tgatcagcaa agccctgata
     1861 aacactgtgc tgaaatacat gtggcagagt gagccctttc gggatgagcc gtggtataat
     1921 cagaaaacgg agtcggaaag gcagaggaat ctgttcctca aggcattcac ggacttcctg
     1981 gccttcatgg tcctctttaa ctacatcatc cctgtgtcca tgtacgtcac ggtcgagatg
     2041 cagaagttcc tcggctctta cttcatcacc tgggacgaag acatgtttga cgaggagact
     2101 ggcgaggggc ctctggtgaa cacgtcggac ctcaatgaag agctgggaca ggtggagtac
     2161 atcttcacag acaagaccgg caccctcacg gaaaacaaca tggagttcaa ggagtgctgc
     2221 atcgaaggcc atgtctacgt gccccacgtc atctgcaacg ggcaggtcct cccagagtcg
     2281 tcaggaatcg acatgattga ctcgtccccc agcgtcaacg ggagggagcg cgaggagctg
     2341 tttttccggg ccctctgtct ctgccacacc gtccaggtga aagacgatga cagcgtagac
     2401 ggccccagga aatcgccgga cggggggaaa tcctgtgtgt acatctcatc ctcgcccgac
     2461 gaggtggcgc tggtcgaagg tgtccagaga cttggcttta cctacctaag gctgaaggac
     2521 aattacatgg agatattaaa cagggagaac cacatcgaaa ggtttgaatt gctggaaatt
     2581 ttgagttttg actcagtcag aaggagaatg agtgtaattg taaaatctgc tacaggagaa
     2641 atttatctgt tttgcaaagg agcagattct tcgatattcc cccgagtgat agaaggcaaa
     2701 gttgaccaga tccgagccag agtggagcgt aacgcagtgg aggggctccg aactttgtgt
     2761 gttgcttata aaaggctgat ccaagaagaa tatgaaggca tttgtaagct gctgcaggct
     2821 gccaaagtgg cccttcaaga tcgagagaaa aagttagcag aagcctatga gcaaatagag
     2881 aaagatctta ctctgcttgg tgctacagct gttgaggacc ggctgcagga gaaagctgca
     2941 gacaccatcg aggccctgca gaaggccggg atcaaagtct gggttctcac gggagacaag
     3001 atggagacgg ccgcggccac gtgctacgcc tgcaagctct tccgcaggaa cacgcagctg
     3061 ctggagctga ccaccaagag gatcgaggag cagagcctgc acgacgtcct gttcgagctg
     3121 agcaagacgg tcctgcgcca cagcgggagc ctgaccagag acaacctgtc cggactttca
     3181 gcagatatgc aggactacgg tttaattatc gacggagctg cactgtctct gataatgaag
     3241 cctcgagaag acgggagttc cggcaactac agggagctct tcctggaaat ctgccggagc
     3301 tgcagcgcgg tgctctgctg ccgcatggcg cccttgcaga aggctcagat tgttaaatta
     3361 atcaaatttt caaaagagca cccaatcacg ttagcaattg gcgatggtgc aaatgatgtc
     3421 agcatgattc tggaagcgca cgtgggcata ggtgtcatcg gcaaggaagg ccgccaggct
     3481 gccaggaaca gcgactatgc aatcccaaag tttaagcatt tgaagaagat gctgcttgtt
     3541 cacgggcatt tttattacat taggatctct gagctcgtgc agtacttctt ctataagaac
     3601 gtctgcttca tcttccctca gtttttatac cagttcttct gtgggttttc acaacagact
     3661 ttgtacgaca ccgcgtatct gaccctctac aacatcagct tcacctccct ccccatcctc
     3721 ctgtacagcc tcatggagca gcatgttggc attgacgtgc tcaagagaga cccgaccctg
     3781 tacagggacg tcgccaagaa tgccctgctg cgctggcgcg tgttcatcta ctggacgctc
     3841 ctgggactgt ttgacgcact ggtgttcttc tttggtgctt atttcgtgtt tgaaaataca
     3901 actgtgacaa gcaacgggca gatatttgga aactggacgt ttggaacgct ggtattcacc
     3961 gtgatggtgt tcacagttac actaaagctt gcattggaca cacactactg gacttggatc
     4021 aaccattttg tcatctgggg gtcgctgctg ttctacgttg tcttttcgct tctctgggga
     4081 ggagtgatct ggccgttcct caactaccag aggatgtact acgtgttcat ccagatgctg
     4141 tccagcgggc ccgcctggct ggccatcgtg ctgctggtga ccatcagcct ccttcccgac
     4201 gtcctcaaga aagtcctgtg ccggcagctg tggccaacag caacagagag agtccagact
     4261 aagagccagt gcctttctgt cgagcagtca accatcttta tgctttctca gacttccagc
     4321 agcctgagtt tcctcgagta cccatacgac gtacctgatt atgcaactta tccttatgat
     4381 gtacctgatt atgctagcta tccttatgat gtcccagact acgcatgagg gcccgtttaa
     4441 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4501 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4561 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4621 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4681 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5161 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5221 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5281 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5341 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5401 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5461 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5521 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5581 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5641 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5701 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5761 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5821 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5881 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5941 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6001 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6061 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6121 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6181 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6241 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6301 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6361 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6421 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6481 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6661 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6721 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6781 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6841 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6901 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6961 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7621 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8881 gatcccctat ggt
//
