LOCUS V029202 7507 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029202 VERSION V029202 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2943 /label="AMER2(NM_152704)" /note="AMER2(NM_152704)" /gene="AMER2" CDS 2950..3039 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 3074..3298 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3344..3772 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3786..4115 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4182..4976 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5150..5271 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5320..5336) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5344..5360 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5368..5398) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5413..5434 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5722..6307) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6478..7338) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7339..7443) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atggagacga gccggagccg cggcggcggc 961 ggggctgtca gcgagcgcgg cggagctggc gcgtccgtgg gggtctgcag gaggaaggcg 1021 gaggccgggg ccgggaccgg gaccctcgcg gcagacatgg acttgcattg tgactgtgcc 1081 gccgaaacgc cggccgccga gccgccgtcg gggaagatta ataaagctgc cttcaaatta 1141 ttcaagaaga ggaaatcggg tggcaccatg cccagcattt ttggggtcaa aaacaaaggg 1201 gacgggaaaa gctcgggtcc gacggggctg gtgaggagca ggacccacga cggacttgcc 1261 gaggtgctgg tgctggagag cggcaggaag gaggagccgc gcggcggggg cgacagcggc 1321 gggggcggcg gggggcggcc gaacccgggg ccccccagag ccgcagggcc cggcgggggc 1381 tccctcgcca gcagctcggt ggccaagtcg cacagcttct tctcgctgct gaagaagaac 1441 gggcgctcgg aaaacggcaa gggagagcct gtggacgcga gcaaggccgg cggcaaacaa 1501 aagcgggggc tgcgggggct gttcagcggc atgcgctggc acaggaaaga caagcgggcc 1561 aaggcggagg ccgcggaggg gcgcgcgccc gggggcggct tgatcctacc cggctcgctc 1621 accgccagcc tggagtgcgt caaggaggag acgcccagag ccgcgcgcga gccggaggag 1681 cccagccagg acgccccgcg agacccagca ggtgagcccg cagggggaga ggaggtgccc 1741 gcccccgccg accgcgcccc agcgcggagc tgccgagagg cagagggcct cgcgcacccc 1801 ggcgacaccg gcgcccgggg agaggacgcc gcggggcatc ggcgcgccga gccggggccc 1861 ggggaggtcc gcacggcaga ggacgcttcc aggacggggg ccgttcccgt aaagacggtc 1921 ccccttgtcg actccgaagg cggcagcggc cgggcgcccg ccgccccaga ccctgcctct 1981 gtcgatccac cctcagaccc gtcggcagat cgtatttgtt tgatgttttc tgacgtgact 2041 tcactgaaaa gctttgactc tcttacaggc tgtggagata ttattgcaga ccaagaggaa 2101 gaggcaggtc ccagctgtga caagcatgtc cccgggccag gcaagccggc tctgtctaaa 2161 aagaaccccg gcgtggtggc ctaccaagga ggcggggaag agatggccag cccggacgag 2221 gtggacgaca cctatctaca ggagttctgg gacatgctct cccagaccga ggagcaggga 2281 cccgagcccc aggagggcgc ggctaaggtg gcagctgcgc tggaaaccaa ggtggtgccc 2341 gagaccccca aagacaccag gtgtgtggaa gcggccaagg acgcgtcctc ggtcaagcgc 2401 aggaggctca accggattcc catcgagccc catcctaagg aggagcccaa gcacccggag 2461 aaggagcagc aggaaggcgt ccccaacagc gacgagggct actgggactc caccacgcca 2521 ggcccagagg aagacagctc gagcagcggg aagaaggcgg gcatcccccg ggatagctac 2581 agcggggacg cgctctatga tctctatgct gacccggacg gaagtccagc aacccttcct 2641 ggagggaagg acaacgagga gacgtcctcc ctgtcccggt taaagcccgt atctccaggc 2701 accatcacct gtccactgcg aacaccaggc agcttgctga aggactctaa gatccctatt 2761 agcatcaagc acctgaccaa ccttccatct agccatcccg tggtgcacca gcaaccctcc 2821 aggagtgaga tgcccagaac aaaaatcccg gtttccaaag tgctggtccg cagagtcagc 2881 aaccggggct tggctgggac caccatcaga gcaacggcct gccacgacag tgccaaaaag 2941 ttggatatcg aacaaaaact aatatcagag gaagatttgg aacagaaact aatcagtgaa 3001 gaagatttag agcaaaagtt aatttccgag gaggacttat gagggcccgt ttaaacccgc 3061 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 3121 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 3181 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 3241 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 3301 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 3361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 3421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 3481 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 3541 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 3601 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 3661 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 3721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 3781 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3841 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 3901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 3961 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 4021 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 4081 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 4141 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 4201 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 4261 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 4321 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 4381 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 4441 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 4501 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 4561 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 4621 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 4681 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 4741 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 4801 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 4861 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 4921 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 4981 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 5041 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 5101 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 5161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 5221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 5281 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 5341 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 5401 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 5461 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 5521 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 5581 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 5641 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5881 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 6001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 6061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 6121 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 6181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 6241 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 6301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 6361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 6421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 6481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 6541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 6601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 6661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 6721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 6781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 6841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 6901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 6961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 7021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 7081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 7141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 7201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 7261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 7321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 7381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 7441 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 7501 ctatggt //