LOCUS V026844 7064 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026844 VERSION V026844 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2554 /label="AJUBA(NM_032876)" /note="AJUBA(NM_032876)" /gene="AJUBA" CDS 2561..2626 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2664..2888 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2934..3362 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3376..3705 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3772..4566 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4740..4861 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4910..4926) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4934..4950 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4958..4988) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5003..5024 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5312..5897) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6068..6928) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6929..7033) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggagcggt taggagagaa 961 agccagtcgc ctgctggaga agttcggccg cagaaagggt gaatctagcc ggtctgggtc 1021 tgacgggacc cccgggccgg gcaaggggcg cctaagtggg ttggggggac ctaggaagtc 1081 agggccccga ggagctactg ggggacctgg ggatgagccg ttggagccgg cccgggagca 1141 aggttccctg gacgctgagc gaaatcagcg cggctccttt gaggcgccgc gctacgaagg 1201 ctcttttccc gcggggccgc cgcccacccg ggccttgcct ctacctcagt cgttgccccc 1261 cgattttcgg ctggagccca cggccccggc cctcagcccc cgctctagct tcgccagtag 1321 ctcggccagc gacgcgagca agccgtccag cccccggggc agcctgctgc tggacggggc 1381 gggggctggc ggagctggag gtagccggcc ctgcagcaat cgcaccagcg gcatcagcat 1441 gggctacgac cagcgccacg ggagcccctt gccagcgggg ccgtgcctgt ttggcccacc 1501 cctggccgga gcaccggcag gctattctcc cggaggggtc ccgtccgcct acccggagct 1561 ccacgccgcc ctggaccgat tgtacgctca gcggcccgcg gggttcggct gccaggaaag 1621 ccgccactcg tatcccccgg ccctgggcag ccctggagct ctagccgggg ccggagtggg 1681 agcggcgggg cccttggaga gacggggggc gcaacccgga cgacactctg tgaccggcta 1741 cggggactgc gccgtgggcg cccggtacca ggacgagcta acagctttgc ttcgcctgac 1801 ggtgggcacc ggtgggcgag aagccggagc ccgcggagaa ccctcgggga ttgagccgtc 1861 gggtctggag gagccaccag gtcctttcgt tccggaggcc gcccgggccc ggatgcggga 1921 gccagaggcc agggaggact acttcggcac ctgtatcaag tgcaacaaag gcatctatgg 1981 gcagagcaat gcctgccagg ccctggacag cctctaccac acccagtgct ttgtttgctg 2041 ctcttgtggg cgaactttgc gttgcaaggc tttctacagt gtcaatggct ctgtgtactg 2101 tgaggaagat tatctgtttt cagggtttca ggaggcagct gagaaatgct gtgtctgtgg 2161 tcacttgatt ttggagaaga tcctacaagc aatggggaag tcctatcatc caggctgttt 2221 ccgatgcatt gtttgcaaca agtgcctgga tggcatcccc ttcacagtgg acttctccaa 2281 ccaagtatac tgtgtcaccg actaccacaa aaattatgct cctaagtgtg cagcctgtgg 2341 ccaacccatc ctcccctctg agggctgtga ggacatcgtg agggtgatat ccatggaccg 2401 ggattatcac tttgagtgct accactgtga ggactgccgg atgcagctga gtgatgagga 2461 aggctgctgc tgtttccctc tggatgggca cttgctctgc catggttgcc acatgcagcg 2521 gctcaatgcc cgacaacccc ctgccaacta tatcctcgag gactacaaag accatgacgg 2581 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccgt 2641 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 2701 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 2761 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 2821 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 2881 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 2941 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 3001 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 3061 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 3121 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 3181 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 3241 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 3301 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 3361 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3421 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3481 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3541 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3601 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 3661 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 3721 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 3781 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 3841 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 3901 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 3961 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 4021 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 4081 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 4141 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 4201 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 4261 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 4321 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 4381 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 4441 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 4501 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 4561 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 4621 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 4681 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 4741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 4801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 4861 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 4921 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 4981 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 5041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 5101 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 5161 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 5221 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 5281 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5341 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 5401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 5521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 5581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 5761 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5821 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca 5881 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 5941 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 6001 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 6061 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 6121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 6241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 6301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 6361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 6421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 6481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 6541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 6601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 6661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 6721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 6781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 6841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 6901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 6961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 7021 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc //