LOCUS       V038745                 9400 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038745
VERSION     V038745
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             907..4920
                     /label="ADGRA2(NM_032777)"
                     /note="ADGRA2(NM_032777)"
                     /gene="ADGRA2"
     polyA_signal    4967..5191
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5237..5665
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5679..6008
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6075..6869
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7043..7164
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7213..7229)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7237..7253
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7261..7291)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7306..7327
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7615..8200)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8371..9231)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9232..9336)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccccatgg gcgccggggg acgcaggatg cggggggcgc ccgcgcgcct gctgctgccg
      961 ctgctgccgt ggctcctgct gctcctggcg cccgaggctc ggggcgcgcc cggctgcccg
     1021 ctatccatcc gcagctgcaa gtgctcgggg gagcggccca aggggctgag cggcggcgtc
     1081 cctggcccgg ctcggcggag ggtggtgtgc agcggcgggg acctcccgga gcctcccgag
     1141 cccggccttc tgcctaacgg caccgttacc ctgctcttga gcaataacaa gatcacgggg
     1201 ctccgcaatg gctccttcct gggactgtca ctgctggaga agctggacct gaggaacaac
     1261 atcatcagca cagtgcagcc gggcgccttc ctgggcctgg gggagctgaa gcgtttagat
     1321 ctctccaaca accggattgg ctgtctcacc tccgagacct tccagggcct ccccaggctt
     1381 ctccgactaa acatatctgg aaacatcttc tccagtctgc aacctggggt ctttgatgag
     1441 ctgccagccc ttaaggttgt ggacttgggc accgagttcc tgacctgtga ctgccacctg
     1501 cgctggctgc tgccctgggc ccagaatcgc tccctgcagc tgtcggaaca cacgctctgt
     1561 gcttacccca gtgccctgca tgctcaggcc ctgggcagcc tccaggaggc ccagctctgc
     1621 tgcgaggggg ccctggagct gcacacacac cacctcatcc cgtccctacg ccaagtggtg
     1681 ttccaggggg atcggctgcc cttccagtgc tctgccagct acctgggcaa cgacacccgc
     1741 atccgctggt accacaaccg agcccctgtg gagggtgatg agcaggcggg catcctcctg
     1801 gccgagagcc tcatccacga ctgcaccttc atcaccagtg agctgacgct gtctcacatc
     1861 ggcgtgtggg cctcaggcga gtgggagtgc accgtgtcca tggcccaagg caacgccagc
     1921 aagaaggtgg agatcgtggt gctggagacc tctgcctcct actgccccgc cgagcgtgtt
     1981 gccaacaacc gcggggactt caggtggccc cgaactctgg ctggcatcac agcctaccag
     2041 tcctgcctgc agtatccctt cacctcagtg cccctgggcg ggggtgcccc gggcacccga
     2101 gcctcccgcc ggtgtgaccg tgccggccgc tgggagccag gggactactc ccactgtctc
     2161 tacaccaacg acatcaccag ggtgctgtac accttcgtgc tgatgcccat caatgcctcc
     2221 aatgcgctga ccctggctca ccagctgcgc gtgtacacag ccgaggccgc tagcttttca
     2281 gacatgatgg atgtagtcta tgtggctcag atgatccaga aatttttggg ttatgtcgac
     2341 cagatcaaag agctggtaga ggtgatggtg gacatggcca gcaacctgat gctggtggac
     2401 gagcacctgc tgtggctggc ccagcgcgag gacaaggcct gcagccgcat cgtgggtgcc
     2461 ctggagcgca ttgggggggc cgccctcagc ccccatgccc agcacatctc agtgaatgcg
     2521 aggaacgtgg cattggaggc ctacctcatc aagccgcaca gctacgtggg cctgacctgc
     2581 acagccttcc agaggaggga gggaggggtg ccgggcacac ggccaggaag ccctggccag
     2641 aaccccccac ctgagcccga gcccccagct gaccagcagc tccgcttccg ctgcaccacc
     2701 gggaggccca atgtttctct gtcgtccttc cacatcaaga acagcgtggc cctggcctcc
     2761 atccagctgc ccccgagtct attctcatcc cttccggctg ccctggctcc cccggtgccc
     2821 ccagactgca ccctgcaact gctcgtcttc cgaaatggcc gcctcttcca cagccacagc
     2881 aacacctccc gccctggagc tgctgggcct ggcaagaggc gtggcgtggc cacccccgtc
     2941 atcttcgcag gaaccagtgg ctgtggcgtg ggaaacctga cagagccagt ggccgtttcg
     3001 ctgcggcact gggctgaggg agccgaacct gtggccgctt ggtggagcca ggaggggccc
     3061 ggggaggctg ggggctggac ctcggagggc tgccagctcc gctccagcca gcccaatgtc
     3121 agcgccctgc actgccagca cttgggcaat gtggccgtgc tcatggagct gagcgccttt
     3181 cccagggagg tggggggcgc cggggcaggg ctgcaccccg tggtataccc ctgcacggcc
     3241 ttgctgctgc tctgcctctt cgccaccatc atcacctaca tcctcaacca cagctccatc
     3301 cgtgtgtccc ggaaaggctg gcacatgctg ctgaacttgt gcttccacat agccatgacc
     3361 tctgctgtct ttgcgggggg catcacactc accaactacc agatggtctg ccaggcggtg
     3421 ggcatcaccc tgcactactc ctccctatcc acgctgctct ggatgggcgt gaaggcgcga
     3481 gtgctccata aggagctcac ctggagggca ccccctccgc aagaagggga ccccgctctg
     3541 cctactccca gtcctatgct ccggttctat ttgatcgctg gagggattcc actcattatc
     3601 tgtggcatca cagctgcagt caacatccac aactaccggg accacagccc ctactgctgg
     3661 ctggtgtggc gtccaagcct tggcgccttc tacatccctg tggctttgat tctgctcatc
     3721 acctggatct atttcctgtg cgccgggcta cgcttacggg gtcctctggc acagaacccc
     3781 aaggcgggca acagcagggc ctccctggag gcaggggagg agctgagggg ttccaccagg
     3841 ctcaggggca gcggccccct cctgagtgac tcaggttccc ttcttgctac tgggagcgcg
     3901 cgagtgggga cgcccgggcc cccggaggat ggtgacagcc tctattctcc gggagtccag
     3961 ctaggggcgc tggtgaccac gcacttcctg tacttggcca tgtgggcctg cggggctctg
     4021 gcagtgtccc agcgctggct gccccgggtg gtgtgcagct gcttgtacgg ggtggcagcc
     4081 tccgccctgg gcctcttcgt cttcactcac cactgtgcca ggcggaggga cgtgagagcc
     4141 tcgtggcgcg cctgctgccc ccctgcctct cccgcggccc cccatgcccc gccccgggcc
     4201 ctgcccgccg ccgcagagga cggttccccg gtgttcgggg agggcccccc ctccctcaag
     4261 tcctccccaa gcggcagcag cggccatccg ctggctctgg gcccctgcaa gctcaccaac
     4321 ctgcagctgg cccagagtca ggtgtgcgag gcgggggcgg cggccggcgg ggaaggagag
     4381 ccggagccgg cgggcacccg gggaaacctc gcccaccgcc accccaacaa cgtgcaccac
     4441 gggcgtcggg cgcacaagag ccgggccaag ggacaccgcg cgggggaggc ctgcggcaag
     4501 aaccggctca aggccctgcg cgggggcgcg gcgggggcgc tggagctgct gtccagcgag
     4561 agcggcagtc tgcacaacag ccccaccgac agctacctgg gcagcagccg caacagcccg
     4621 ggcgccggcc tgcagctgga aggcgagccc atgctcacgc cgtccgaggg cagcgacacc
     4681 agcgccgcgc cgctttctga ggcgggccgg gcaggccagc gccgcagcgc cagccgcgac
     4741 agtctcaagg gcggcggcgc gctggagaag gagagccatc gccgctcgta cccgctcaac
     4801 gccgccagcc taaacggcgc ccccaagggg ggcaagtacg acgacgtcac cctgatgggc
     4861 gcggaggtag ccagcggcgg ctgcatgaag accggactct ggaagagcga aactaccgtc
     4921 taactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4981 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5041 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5101 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5161 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5221 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5281 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5341 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5401 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5461 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5521 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5581 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5641 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     5701 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5761 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5821 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5881 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5941 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6001 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6061 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6121 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6181 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6241 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6301 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6361 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6421 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6481 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6541 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6601 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6661 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6721 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6781 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6841 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     6901 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6961 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7021 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7081 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7141 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7201 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7261 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7321 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7381 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7441 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7501 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7561 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7621 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7681 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7741 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7801 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7861 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7921 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7981 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8041 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8101 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     8161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8281 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8341 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8401 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8461 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8521 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8581 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8641 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8701 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8761 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8821 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8881 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8941 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9001 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9061 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9121 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9181 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9241 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9301 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9361 tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
