LOCUS       V031566                 7694 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031566
VERSION     V031566
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3214
                     /label="ADAM11(NM_002390)"
                     /note="ADAM11(NM_002390)"
                     /gene="ADAM11"
     polyA_signal    3261..3485
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3531..3959
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3973..4302
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4369..5163
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5337..5458
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5507..5523)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5531..5547
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5555..5585)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5600..5621
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5909..6494)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6665..7525)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7526..7630)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aggctgctgc ggcgctgggc gttcgcggct ctgctgctgt cgctgctccc
      961 cacgcccggt cttgggaccc aaggtcctgc tggagctctg cgatgggggg gcttacccca
     1021 gctgggaggc ccaggagccc ctgaggtcac ggaacccagc cgtctggtta gggagagctc
     1081 cgggggagag gtccgaaagc agcagctgga cacaagggtc cgccaggagc caccaggggg
     1141 cccgcctgtc catctggccc aggtgagttt cgtcatccca gccttcaact caaacttcac
     1201 cctggacctg gagctgaacc accacctcct ctcctcgcaa tacgtggagc gccacttcag
     1261 ccgggagggg acaacccagc acagcaccgg ggctggagac cactgctact accaggggaa
     1321 gctccggggg aacccgcact ccttcgccgc cctctccacc tgccaggggc tgcatggggt
     1381 cttctctgat gggaacttga cttacatcgt ggagccccaa gaggtggctg gaccttgggg
     1441 agcccctcag ggaccccttc cccacctcat ttaccggacc cctctcctcc cagatcccct
     1501 cggatgcagg gaaccaggct gcctgtttgc tgtgcctgcc cagtcggctc ctccaaaccg
     1561 gccgaggctg agaaggaaaa ggcaggtccg ccggggccac cctacagtgc acagtgaaac
     1621 caagtatgtg gagctaattg tgatcaacga ccaccagctg ttcgagcaga tgcgacagtc
     1681 ggtggtcctc accagcaact ttgccaagtc cgtggtgaac ctggccgatg tgatatacaa
     1741 ggagcagctc aacactcgca tcgtcctggt tgccatggaa acatgggcag atggggacaa
     1801 gatccaggtg caggatgacc tcctggagac cctggcccgg ctcatggtct accgacggga
     1861 gggtctgcct gagcccagtg atgccaccca cctcttctcg ggcaggacct tccagagcac
     1921 gagcagcggg gcagcctacg tggggggcat atgctccctg tcccacggcg ggggtgtgaa
     1981 cgagtacggc aacatggggg cgatggccgt gacccttgcc cagacgctgg gacagaacct
     2041 gggcatgatg tggaacaaac accggagctc ggcaggggac tgcaagtgtc cagacatctg
     2101 gctgggctgc atcatggagg acactgggtt ctacctgccc cgcaagttct cgcgctgtag
     2161 catcgacgag tacaaccagt ttctgcagga gggtggtggc agctgcctct tcaacaagcc
     2221 cctcaagctc ctggaccccc cagagtgcgg gaacggcttc gtggaggcag gggaggagtg
     2281 cgactgcggc tcggtgcagg agtgcagccg cgcaggtggc aactgctgca agaaatgcac
     2341 cctgactcac gacgccatgt gcagcgacgg gctctgctgt cgccgctgca agtacgaacc
     2401 acggggtgtg tcctgccgag aggccgtgaa cgagtgcgac atcgcggaga cctgcaccgg
     2461 ggactctagc cagtgcccgc ctaacctgca caagctggac ggttactact gtgaccatga
     2521 gcagggccgc tgctacggag gtcgctgcaa aacccgggac cggcagtgcc aggttctttg
     2581 gggccatgcg gctgctgatc gcttctgcta cgagaagctg aatgtggagg ggacggagcg
     2641 tgggagctgt gggcgcaagg gatctggctg ggtccagtgc agtaagcagg acgtgctgtg
     2701 tggcttcctc ctctgtgtca acatctctgg agctcctcgg ctaggggacc tggtgggaga
     2761 catcagtagt gtcaccttct accaccaggg caaggagctg gactgcaggg gaggccacgt
     2821 gcagctggcg gacggctctg acctgagcta tgtggaggat ggcacagcct gcgggcctaa
     2881 catgttgtgc ctggaccatc gctgcctgcc agcttctgcc ttcaacttca gcacctgccc
     2941 cggcagtggg gagcgccgga tttgctccca ccacggggtc tgcagcaatg aagggaagtg
     3001 catctgtcag ccagactgga caggcaaaga ctgcagtatc cataaccccc tgcccacgtc
     3061 cccacccacg ggggagacgg agagatataa aggtcccagc ggcaccaaca tcatcattgg
     3121 ctccattgct ggggctgtcc tggttgcagc catcgtcctg ggcggcacgg gctggggatt
     3181 taaaaacatt cgccgaggaa ggtccggagg ggcctaactc gagtctagag ggcccgttta
     3241 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3301 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3361 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3421 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     3481 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     3541 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3601 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3661 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3721 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3781 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3841 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3901 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     3961 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4021 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4141 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4201 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     4261 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     4321 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     4381 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     4441 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     4501 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     4561 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     4621 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     4681 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     4741 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     4801 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     4861 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     4921 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     4981 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     5041 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     5101 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     5161 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     5221 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     5281 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     5341 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     5461 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     5521 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5581 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5641 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5701 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6421 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6481 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6541 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6601 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6661 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6721 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6781 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6841 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6901 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6961 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7021 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7081 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7141 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7201 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7261 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7321 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7381 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7441 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7501 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7561 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7621 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     7681 cgatccccta tggt
//
