LOCUS       V038933                 8457 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038933
VERSION     V038933
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             927..3260
                     /label="ABLIM1(NM_002313)"
                     /note="ABLIM1(NM_002313)"
                     /gene="ABLIM1"
     CDS             3300..4019
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4057..4281
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4327..4755
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4769..5098
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5165..5959
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6133..6254
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6303..6319)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6327..6343
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6351..6381)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6396..6417
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6705..7290)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7461..8321)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8322..8426)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaagcc actgggatcc gccaccatgc ctgccttcct tggtctaaag tgtctgggga
      961 aattgtgcag ctctgagaaa agcaaagtca cctcatctga gagaaccagt gccaggggct
     1021 cgaacagaaa gagactgatt gttgaggacc ggagggtctc tgggacctcc ttcaccgctc
     1081 ataggcgtgc cactatcact catttgctgt atctctgtcc caaggactac tgcccacgtg
     1141 ggcgtgtatg taacagcgtt gatccttttg tggcccaccc tcaggaccct caccacccat
     1201 cagagaagcc tgtcattcac tgccataaat gtggggagcc ttgcaagggt gaagtgcttc
     1261 gggtccagac caaacatttc cacatcaagt gtttcacctg caaagtgtgt ggctgtgacc
     1321 tggcacaagg gggcttcttc ataaagaacg gagagtatct ctgcaccctg gactaccagc
     1381 ggatgtacgg gacacgctgc catggctgtg gggagttcgt ggagggcgaa gtggtgactg
     1441 ctctgggcaa gacctaccat cccaattgct ttgcttgtac tatctgcaag cgcccgtttc
     1501 cacccggaga ccgagtcaca ttcaatggga gagactgcct ttgtcaactc tgtgcacagc
     1561 cgatgtcgtc cagtccgaaa gaaaccacct tctccagcaa ttgtgccggc tgcggaagag
     1621 atatcaagaa tgggcaggcg ctgctggcgc tggataagca gtggcacttg gggtgcttta
     1681 aatgcaagtc ctgcgggaag gtcctcaccg gggagtacat cagcaaggat ggtgctccgt
     1741 actgtgaaaa ggactaccag ggactctttg gggtgaaatg tgaggcgtgt caccagttta
     1801 tcacagggaa agtcctggag gcaggtgaca aacattacca ccccagctgt gcacgatgca
     1861 gcagatgcaa ccagatgttc acagaaggag aggaaatgta tcttcaaggc tccaccgttt
     1921 ggcatcccga ctgtaagcaa tctacgaaga ccgaggaaaa gctgcggcct accaggacat
     1981 cctcggaaag tatttattct aggccaggct ccagtattcc tggctcacca ggtcatacta
     2041 tctatgcaaa agtagacaat gagatcctgg attacaagga tttagcagcc attccgaagg
     2101 tcaaggcaat ttatgacatt gaacgtccag atcttattac ctatgagcct ttctacactt
     2161 cgggctatga tgacaaacag gagagacaga gccttggaga gtctccgagg actttgtctc
     2221 ctactccatc agcagaaggg taccaggatg ttcgggatcg gatgatccat cggtccacga
     2281 gccagggctc catcaactcc cctgtgtaca gccgccacag ctacactcca accacgtccc
     2341 gctctcccca gcatttccac agacctggca atgagccgtc cagcggccgg aactcccctc
     2401 tcccttaccg gccagacagc cgccctctaa ctccaactta cgctcaggcc cctaaacatt
     2461 tccatgttcc agatcaagga atcaacattt accgaaagcc acccatctac aaacagcatg
     2521 ctgccttggc agcccagagc aagtcctcag aagatatcat caagttttcc aagttcccag
     2581 cagcccaggc accagacccc agcgagacac caaagattga gacggaccac tggcctggtc
     2641 ccccctcatt tgctgtcgta ggacctgaca tgaaacgcag atctagtggc agagaggaag
     2701 atgatgagga acttctgaga cgtcggcagc ttcaagaaga gcaattaatg aagcttaact
     2761 caggcctggg acagttgatc ttgaaagaag agatggagaa agagagccgg gaaaggtcat
     2821 ctctgttagc cagtcgctac gattctccca tcaactcagc ttcacatatt ccatcatcta
     2881 aaactgcatc tctccctggc tatggaagaa atgggcttca ccggcctgtt tctaccgact
     2941 tcgctcagta taacagctat ggggatgtca gcgggggagt gcgagattac cagacactcc
     3001 cagatggcca catgcctgca atgagaatgg accgaggagt gtctatgccc aacatgttgg
     3061 aaccaaagat atttccatat gaaatgctca tggtgaccaa cagagggcga aacaaaatcc
     3121 tcagagaggt ggacagaacc aggctggagc gccacttagc ccctgaagtg tttcgggaaa
     3181 tctttggaat gtccatacag gagtttgaca ggttacctct ttggagacgc aacgacatga
     3241 agaaaaaagc aaaactcttc tcctcgagtg gcggtggagg aagcggcgga ggtggatcta
     3301 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3361 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3421 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3481 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3541 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3601 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3661 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3721 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3781 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3841 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3901 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3961 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtagt
     4021 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4081 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4141 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4201 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4261 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4321 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4381 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4441 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4501 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4561 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4621 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4681 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4741 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4801 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4861 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4921 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4981 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5041 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5101 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     5161 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5221 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5281 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5341 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5401 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5461 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5521 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5581 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5641 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5701 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5761 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5821 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     5881 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     5941 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6001 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6061 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6121 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6241 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag
     7261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     7441 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7501 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7561 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7621 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7681 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7741 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7801 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7861 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7921 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7981 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8041 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8101 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8161 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8221 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8281 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8341 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8401 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
