LOCUS V040747 8377 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040747 VERSION V040747 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..3867 /label="AARS1(NM_001605)" /note="AARS1(NM_001605)" /gene="AARS1" CDS 3874..3939 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3977..4201 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4247..4675 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4689..5018 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5085..5879 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6053..6174 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6223..6239) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6247..6263 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6271..6301) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6316..6337 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6625..7210) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7381..8241) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8242..8346) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatggact ctactctaac agcaagtgaa atccggcagc gatttataga tttcttcaag 1021 aggaacgagc atacgtatgt tcactcgtct gccaccatcc cattggatga ccccactttg 1081 ctctttgcca atgcaggcat gaaccagttt aaacccattt tcctgaacac aattgaccca 1141 tctcacccca tggcaaagct gagcagagct gccaataccc agaagtgcat ccgggctggg 1201 ggcaaacata atgacctgga cgatgtgggc aaggatgtct atcatcacac cttcttcgag 1261 atgctgggct cttggtcttt tggagattac tttaaggaat tggcatgtaa gatggctctg 1321 gaactcctca cccaagagtt tggcattccc attgaaagac tttatgttac ttactttggc 1381 ggggatgaag cagctggctt agaagcagat ctggaatgca aacagatctg gcaaaatttg 1441 gggctggatg acaccaaaat cctcccaggc aacatgaagg ataacttctg ggagatgggt 1501 gacacgggcc cctgtggtcc ttgcagtgag atccactacg accggattgg tggtcgggac 1561 gccgcacatc ttgtcaacca ggacgaccct aatgtgctgg agatctggaa ccttgtgttc 1621 atccagtata acagggaagc tgatggcatt ctgaaacctc ttcccaagaa aagcattgac 1681 acagggatgg gcctggaacg actggtatct gtgctgcaga ataagatgtc caactatgac 1741 actgaccttt ttgtccctta ctttgaagcc attcagaagg gcacaggtgc ccgaccatac 1801 actgggaaag ttggtgctga ggatgccgat gggattgaca tggcctaccg ggtgctggct 1861 gaccacgctc ggaccatcac tgtggcactg gctgatggtg gccggcctga caacacaggg 1921 cgtggatatg tgttgagacg gattctccgc cgagctgtcc gatacgccca tgaaaagctc 1981 aatgccagca ggggcttctt tgctacgtta gtggatgttg tcgtccagtc cctgggagat 2041 gcatttcctg agctgaagaa ggacccagac atggtgaagg acatcattaa tgaagaagag 2101 gtgcagtttc tcaagactct cagcagaggg cgtcgcatcc tggacaggaa aattcagagc 2161 ctgggagaca gcaagaccat tcccggagac actgcttggc tcctctatga cacctatggg 2221 tttccagtgg atctgactgg actgattgct gaagagaagg gcctggtggt agacatggat 2281 ggctttgaag aggagaggaa actggcccag ctgaaatcac agggcaaggg agctggtggg 2341 gaagacctca ttatgctgga catttacgct atcgaagagc tccgggcacg gggtctggag 2401 gtcacagatg attccccaaa gtacaattac catttggact ccagtggtag ctatgtattt 2461 gagaacacag tggctacggt gatggctctg cgcagggaga agatgttcgt ggaagaggtg 2521 tccacaggcc aggagtgtgg agtggtgctg gacaagacct gtttctatgc tgagcaagga 2581 ggccagatct atgacgaagg ctacctggtg aaggtggatg acagcagtga agataaaaca 2641 gagtttacag tgaagaatgc tcaggtccga ggagggtatg tgctacacat tggaaccatc 2701 tacggtgacc tgaaagtggg ggatcaggtc tggctgttta ttgatgagcc ccgacgaaga 2761 cccatcatga gcaaccacac agctacgcac attctgaact tcgccctgcg ctcagtgctt 2821 ggggaagctg accagaaagg ctcattggtt gctcctgacc gcctcagatt tgactttact 2881 gccaagggag ccatgtccac ccaacagatc aagaaggctg aagagattgc taatgagatg 2941 attgaggcag ccaaggccgt ctatacccag gattgccccc tggcagcagc gaaagccatc 3001 cagggcctac gggctgtgtt tgatgagacc tatcctgacc ctgtgcgagt cgtctccatt 3061 ggggtcccgg tgtccgagtt gctggatgac ccctctgggc ctgctggctc cctgacttct 3121 gttgagttct gtgggggaac gcacctgcgg aactcgagtc atgcaggagc ttttgtgatc 3181 gtgacggaag aagccattgc caagggtatc cggaggattg tggctgtcac aggtgccgag 3241 gcccagaagg ccctcaggaa agcagagagc ttgaagaaat gtctctctgt catggaagcc 3301 aaagtgaagg ctcagactgc tccaaacaag gatgtgcaga gggagatcgc tgaccttgga 3361 gaggccctgg ccactgcagt catcccccag tggcagaagg atgaattgcg ggagactctc 3421 aaatccctaa agaaggtcat ggatgacttg gaccgagcca gcaaagccga tgtccagaaa 3481 cgagtgttag agaagacgaa gcagttcatc gacagcaacc ccaaccagcc tcttgtcatc 3541 ctggagatgg agagcggcgc ctcagccaag gccctgaatg aagccttgaa gctcttcaag 3601 atgcactccc ctcagacttc tgccatgctc ttcacggtgg acaatgaggc tggcaagatc 3661 acgtgcctgt gtcaagttcc ccagaatgca gccaatcggg gcttaaaagc cagcgagtgg 3721 gtgcagcagg tgtcaggctt gatggacggt aaaggtggtg gcaaggatgt gtctgcacag 3781 gccacaggca agaacgttgg ctgcctgcag gaggcgctgc agctggccac ttccttcgcc 3841 cagctgcgcc tcggggatgt aaagaacctc gaggactaca aagaccatga cggtgattat 3901 aaagatcatg acatcgacta caaggatgac gatgacaagt agtgagggcc cgtttaaacc 3961 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 4021 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 4081 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 4141 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 4201 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 4261 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 4321 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 4381 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 4441 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 4501 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 4561 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 4621 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 4681 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 4741 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 4801 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4861 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4921 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 4981 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 5041 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 5101 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 5161 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 5221 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 5281 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 5341 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 5401 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 5461 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 5521 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 5581 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 5641 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 5701 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 5761 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 5821 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 5881 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 5941 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 6001 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 6061 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6121 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6181 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 6241 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6301 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6361 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6421 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6481 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6541 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6601 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6661 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6721 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 6781 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 6841 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6901 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 6961 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7021 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7081 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7141 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 7741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 7801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 7861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 7921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 7981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 8281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8341 tccgcgcaca tttccccgaa aagtgccacc tgacgtc //