LOCUS       40924_10076        7063 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pcDNA3.1+PA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Vahedi F, Taiebi Meibody N, KianiZadeh M, Mahmoudi M.
  TITLE     Construction of a Eukaryotic Plasmid Encoding Bacillus anthracis 
            Protective Antigen, a Candidate for DNA Vaccine
  JOURNAL   Iran J Immunol 2, 134-140 (2005)
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Vahedi F, Kyd J, Julla GM, Afzalaghaeei M, Kianizadeh M, Mahmoudi M.
  TITLE     A DNA vaccine candidate for B. anthracis immunization, pcDNA3.1+PA 
            plasmid, induce Th1/Th2 mixed responses and protection in mice
  JOURNAL   World J. Microbiol. Biotechnol. 24 (10), 2171-2178 (2008)
REFERENCE   3  (bases 1 to 7063)
  AUTHORS   Vahedi F, Mahmoudi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2007) Immunology, Razi Vaccine and Serum 
            Institute, Ahmad Abad, Mashhad 91735, Iran
REFERENCE   4  (bases 1 to 7063)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7063)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Iran J 
            Immunol 2, 134-140 (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "World J. 
            Microbiol. Biotechnol."; date: "2008"; volume: "24"; issue: "10"; 
            pages: "2171-2178"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-APR-2007) Immunology, Razi Vaccine and Serum Institute, Ahmad 
            Abad, Mashhad 91735, Iran"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            920..2605
                     /gene="pa"
                     /label=pa
                     /note="protective antigen fragment; derived from Bacillus 
                     anthracis isolate 34F2 plasmid pXO1"
     polyA_signal    2663..2887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2933..3361
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3375..3704
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3771..4562
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4739..4872
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4909..4925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4933..4949)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4957..4987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5002..5023)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5311..5896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6070..6927)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6928..7032)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca tggttccaga ccgtgacaat gatggaatcc ctgattcatt
      961 agaggtagaa ggatatacgg ttgatgtcaa aaataaaaga acttttcttt caccatggat
     1021 ttctaatatt catgaaaaga aaggattaac caaatataaa tcatctcctg aaaaatggag
     1081 cacggcttct gatccgtaca gtgatttcga aaaggttaca ggacggattg ataagaatgt
     1141 atcaccagag gcaagacacc cccttgtggc agcttatccg attgtacatg tagatatgga
     1201 gaatattatt ctctcaaaaa atgaggatca atccacacag aatactgata gtcaaacgag
     1261 aacaataagt aaaaatactt ctacaagtag gacacatact agtgaagtac atggaaatgc
     1321 agaagtgcat gcgtcgttct ttgatattgg tgggagtgta tctgcaggat ttagtaattc
     1381 gaattcaagt acggtcgcaa ttgatcattc actatctcta gcaggggaaa gaacttgggc
     1441 tgaaacaatg ggtttaaata ccgctgatac agcaagatta aatgccaata ttagatatgt
     1501 aaatactggg acggctccaa tctacaacgt gttaccaacg acttcgttag tgttaggaaa
     1561 aaatcaaaca ctcgcgacaa ttaaagctaa ggaaaaccaa ttaagtcaaa tacttgcacc
     1621 taataattat tatccttcta aaaacttggc gccaatcgca ttaaatgcac aagacgattt
     1681 cagttctact ccaattacaa tgaattacaa tcaatttctt gagttagaaa aaacgaaaca
     1741 attaagatta gatacggatc aagtatatgg gaatatagca acatacaatt ttgaaaatgg
     1801 aagagtgagg gtggatacag gctcgaactg gagtgaagtg ttaccgcaaa ttcaagaaac
     1861 aactgcacgt atcattttta atggaaaaga tttaaatctg gtagaaaggc ggatagcggc
     1921 ggttaatcct agtgatccat tagaaacgac taaaccggat atgacattaa aagaagccct
     1981 taaaatagca tttggattta acgaatcgaa tggaaactta caatatcaag ggaaagacat
     2041 aaccgaattt gattttaatt tcgatcaaca aacatctcaa aatatcaaga atcagttagc
     2101 ggaattaaac gtaactaaca tatatactgt attagataaa atcaaattaa atgcaaaaat
     2161 gaatatttta ataagagata aacgttttca ttatgataga aataacatag cagttggggc
     2221 ggatgagtca gtagttaagg aggctcatag agaagtaatt aattcgtcaa cagagggatt
     2281 attgttaaat attgataagg atataagaaa aatattatca ggttatattg tagaaattga
     2341 agatactgaa gggcttaaag aagttataaa tgacagatat gatatgttga atatttctag
     2401 tttacggcaa gatggaaaaa catttataga ttttaaaaaa tataatgata aattaccgtt
     2461 atatataagt aatcccaatt ataaggtaaa tgtatatgct gttactaaag aaaacactat
     2521 tattaatcct agtgagaatg gggatactag taccaacggg atcaagaaaa ttttaatctt
     2581 ttctaaaaaa ggctatgaga taggataagg taattgaagc tcgagtctag agggcccgtt
     2641 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2701 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2761 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2821 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2881 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2941 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3001 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3061 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3121 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3181 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3241 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3301 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3361 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3421 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     3481 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     3541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     3661 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     3721 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     3781 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3841 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3901 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3961 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4021 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4081 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4141 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4201 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4261 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     4321 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4381 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4441 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4501 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4561 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4621 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4681 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     4741 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4801 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4861 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     4921 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4981 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5821 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     5881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7021 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
