LOCUS       V008595                 7476 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008595
VERSION     V008595
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7476)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7476)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             947..3082
                     /gene="IRAK1"
                     /label="Interleukin-1 receptor-associated kinase 1"
                     /note="Interleukin-1 receptor-associated kinase 1 from Homo
                     sapiens. Accession#: P51617"
     promoter        complement(3101..3119)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3145..3369
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3415..3843
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3896..4106
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             4181..4972
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5149..5282
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5319..5335)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5343..5359)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5367..5397)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5412..5433)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5721..6309)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6483..7340)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7341..7445)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcaccatgg ccggggggcc
      961 gggcccgggg gagcccgcag cccccggcgc ccagcacttc ttgtacgagg tgccgccctg
     1021 ggtcatgtgc cgcttctaca aagtgatgga cgccctggag cccgccgact ggtgccagtt
     1081 cgccgccctg atcgtgcgcg accagaccga gctgcggctg tgcgagcgct ccgggcagcg
     1141 cgcggccagc gtcctgtggc cctggatcaa ccgcaacgcc cgtgtggccg acctcgtgca
     1201 catcctcacg cacctgcagc tgctccgtgc gcgggacatc atcacagcct ggcaccctcc
     1261 cgccccgctt ccgtccccag gcaccactgc cccgaggccc agcagcatcc ctgcacccgc
     1321 cgaggccgag gcctggagcc cccggaagtt gccatcctca gcctccacct tcctctcccc
     1381 agcttttcca ggctcccaga cccattcagg gcctgagctc ggcctggttc caagccctgc
     1441 ttccctgtgg cctccaccgc catctccagc cccttcttct accaagccag gcccagagag
     1501 ctcagtgtcc ctcctgcagg gagcccgccc ctctccgttt tgctggcccc tctgtgagat
     1561 ttcccggggc acccacaact tctcggagga gctcaagatc ggggagggtg gctttgggtg
     1621 cgtgtaccgg gcggtgatga ggaacacggt gtatgctgtg aagaggctga aggagaacgc
     1681 tgacctggag tggactgcag tgaagcagag cttcctgacc gaggtggagc agctgtccag
     1741 gtttcgtcac ccaaacattg tggactttgc tggctactgt gctcagaacg gcttctactg
     1801 cctggtgtac ggcttcctgc ccaacggctc cctggaggac cgtctccact gccagaccca
     1861 ggcctgccca cctctctcct ggcctcagcg actggacatc cttctgggta cagcccgggc
     1921 aattcagttt ctacatcagg acagccccag cctcatccat ggagacatca agagttccaa
     1981 cgtccttctg gatgagaggc tgacacccaa gctgggagac tttggcctgg cccggttcag
     2041 ccgctttgcc gggtccagcc ccagccagag cagcatggtg gcccggacac agacagtgcg
     2101 gggcaccctg gcctacctgc ccgaggagta catcaagacg ggaaggctgg ctgtggacac
     2161 ggacaccttc agctttgggg tggtagtgct agagaccttg gctggtcaga gggctgtgaa
     2221 gacgcacggt gccaggacca agtatctgaa agacctggtg gaagaggagg ctgaggaggc
     2281 tggagtggct ttgagaagca cccagagcac actgcaagca ggtctggctg cagatgcctg
     2341 ggctgctccc atcgccatgc agatctacaa gaagcacctg gaccccaggc ccgggccctg
     2401 cccacctgag ctgggcctgg gcctgggcca gctggcctgc tgctgcctgc accgccgggc
     2461 caaaaggagg cctcctatga cccaggtgta cgagaggcta gagaagctgc aggcagtggt
     2521 ggcgggggtg cccgggcatt tggaggccgc cagctgcatc cccccttccc cgcaggagaa
     2581 ctcctacgtg tccagcactg gcagagccca cagtggggct gctccatggc agcccctggc
     2641 agcgccatca ggagccagtg cccaggcagc agagcagctg cagagaggcc ccaaccagcc
     2701 cgtggagagt gacgagagcc taggcggcct ctctgctgcc ctgcgctcct ggcacttgac
     2761 tccaagctgc cctctggacc cagcacccct cagggaggcc ggctgtcctc agggggacac
     2821 ggcaggagaa tcgagctggg ggagtggccc aggatcccgg cccacagccg tggaaggact
     2881 ggcccttggc agctctgcat catcgtcgtc agagccaccg cagattatca tcaaccctgc
     2941 ccgacagaag atggtccaga agctggccct gtacgaggat ggggccctgg acagcctgca
     3001 gctgctgtcg tccagctccc tcccaggctt gggcctggaa caggacaggc aggggcccga
     3061 agaaagtgat gaatttcaga gctgatctag agggccctat tctatagtgt cacctaaatg
     3121 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3181 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3241 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3301 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3361 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3481 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3541 ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt
     3601 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3721 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3781 ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3841 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3961 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4021 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     4081 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     4141 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     4381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4681 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     4741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4981 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5041 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5101 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     5161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     5281 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     5341 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5401 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5461 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5521 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5581 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5881 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     5941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6121 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6481 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6541 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6601 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6661 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6721 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6781 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6841 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6901 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6961 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7021 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7081 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7141 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7201 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7261 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7321 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7381 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7441 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
