LOCUS       V008597                 8223 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008597
VERSION     V008597
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     A recombinant plasmid for specific expression of human factor IX in
            kertinocytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Hosseini SJ, Zomorodipour A, Jalal R, Sabouni F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2006) Molecular Genetics, The National Institute
            for Genetic Engineering and Biotechnology, Blvd. Pjoohesh. km-17
            Tehran-karaj Highway, Tehran 14155-6343, Iran
REFERENCE   3  (bases 1 to 8223)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-AUG-2006) Molecular Genetics, The National Institute for Genetic
            Engineering and Biotechnology, Blvd. Pjoohesh. km-17 Tehran-karaj
            Highway, Tehran 14155-6343, Iran"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(260..277)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             2285..3667
                     /gene="F9"
                     /label="Coagulation factor IX"
                     /note="Coagulation factor IX from Homo sapiens. Accession#:
                     P00740"
     promoter        complement(3775..3793)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3819..4043
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4089..4517
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4531..4861
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4928..5719
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5896..6029
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6066..6082)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6090..6106)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6114..6144)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6159..6180)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6468..7056)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7230..8087)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8088..8192)
                     /label="AmpR promoter"
     promoter        8223
                     /label="K14"
                     /note="Human keratin 14 promoter"
ORIGIN
        1 gacggatcgg gagatctgct agggttctgg tgttggtgcg tggggttggg gtgggactgc
       61 agaagtgcct tttaagatta tgtgattgac tgatctgtca ttggttccct gccatcttta
      121 tcttttggat tcccctcgga ggagggggag gaaggagttt cttttgggtt ttattgaatc
      181 aaatgaaagg gaaagtagag gtgttcctat gtcccgggct ccggagcttc tattcctgat
      241 ccctgcataa gaaggagaca tggtggtggt ggtggtgggt gggggtggtg gggcacagag
      301 gaagccgata ctgggctctg caccccattc ccgctcccag atccctctgg acacagcatt
      361 tttctccagt gagcacagcc tccccttgcc ccacagccaa cagcaacatg cctcccaaca
      421 aaagcatctg tccctcagcc aaaacccctg ttgcctctct ctggggaaat tgtagggctg
      481 ggccagggtg gggggaccat tctctgcagg gagattagga gtktctgtca ggggcgggtg
      541 gagcggggtg gggccctggc ttactcacat ccttgagagt cctttgctgg cagatttggg
      601 gagcccacag ctcagatgtc tgtctcagca ttgtcttcca agctcctagg ccacagtagt
      661 ggggggctcc cttctctggc ttcttctttg gtgacagtca aggtggggtt gggggtgaca
      721 gagggtcctg cttctcttct aggagcagtt gatcccagga agagcattgg agcctccagc
      781 aggggctgtt ggggcctgtc tgaggagata ggatgcgtca ggcagcccca gacacgatca
      841 cattcctctc aacatgcctg ccggggtctg tggagccgag gggctgatgg gagggtgggg
      901 tgggggccgg aagggtttgc tttgggaggt tgtctgggag attgctgaag ttttgatata
      961 cacacctcca aagcaggacc aagtggactc ctacccctga cccttggggc ttcaggagtc
     1021 agggaccctc gtgtccacct cagccttgcc cttggcacag cccagctcca ctccagcctc
     1081 tactcctccc cagaacatct cctgggccag ttccacaagg ggctcaaacg agggcacctg
     1141 agctgcccac actagggatg ttctgggggt ctgagaagat atctggggct ggaagaataa
     1201 aaggcccccc taggcctgtt cctggatgca gctccagcca ctttggggct aagcctgggc
     1261 tataacaatg ccaacgaggc ctcttgccat actcggttta caaaaccctt tcacatacat
     1321 tgtcgcattg gattctcaga gctgactgca ctaagcagaa tagatggtat gactcccact
     1381 ttgcagatga gaacactgag gctcagagaa gtgccaagcc ctgggtcaca gaggcgtaaa
     1441 tggcagagcc aggacccacc tgactccagg ctgtttcctg gcctccatga ggccacctgc
     1501 cctatggtgt ggtggatgtg agatcctcac catagggagg agattagggt ctgtgctcag
     1561 ggctggggag agctgcctgg atttctcttt gatggggatg ttggggtggg aatcacgata
     1621 cacctgacta gctgggtgta tttcagggat gggacagact tctcagcaca gcacggcagg
     1681 tcaggcctgg gagggccccc cagacctcct tgtctctaat agagggtcat ggtgagggag
     1741 gcctgtctgt gcccaaggtg accttgccat gccggtgctt tccagccggg tatccatccc
     1801 ctgcagcagc aggcttcctc tacgtggatg ttaaaggccc attcagttca tggagagcta
     1861 gcaggtaact aggtttaagg tgcagaggcc ctgctctctg tcaccctggc taagcccagt
     1921 gcgtgggttc ctgagggctg ggactcccag ggtccgatgg gaaagtgtag cctgcaggcc
     1981 cacacctccc cctgtgaatc acgcctggcg ggacaagaaa gcccaaaaca ctccaaacaa
     2041 tgagtttcca gtaaaatatg acagacatga tgaggcggat gagaggaggg acctggctgg
     2101 gagttggcgc tagcctgtgg gtgatgaaag ccaaggggaa tggaaagtgc cagacccgcc
     2161 ccctacccat gagtataaag cactcgcatc cctttccaat ttacccgagc accttctctt
     2221 cactcagcca actgctgcgt cgctcacctc cctcctcaag cttggtaccg agctcggatc
     2281 cgttatgcag cgcgtgaaca tgatcatggc agaatcacca ggcctcatca ccatctgcct
     2341 tttaggatat ctactcagtg ctgaatgtac agtttttctt gatcatgaaa acgccaacaa
     2401 aattctgaat cggccaaaga ggtataattc aggtaaattg gaagagtttg ttcaagggaa
     2461 ccttgagaga gaatgtatgg aagaaaagtg tagttttgga gaagcacgag aagtttttga
     2521 aaacactgaa agaacaactg aattttggaa gcagtatgtt gatggagatc agtgtgagtc
     2581 caatccatgt ttaaatggcg gcagttgcaa ggatgacatt aattcctatg aatgttggtg
     2641 tccctttgga tttgaaggaa agaactgtga attagatgta acatgtaaca ttaagaatgg
     2701 cagatgcgag cagttttgta aaaatagtgc tgataacaag gtggtttgct cctgtactga
     2761 gggatatcga cttgcagaaa accagaagtc ctgtgaacca gcagtgccat ttccatgtgg
     2821 aagagtttct gtttcacaaa cttctaagct cacccgtgct gagactgttt ttcctgatgt
     2881 ggactatgta aattctactg aagctgaaac cattttggat aacatcactc aaagcaccca
     2941 atcatttaat gacttcactc gggttgttgg tggagaagat gccaaaccag gtcaattccc
     3001 ttggcaggtt gttttgaatg gtaaagttga tgcattctgt ggaggctcta tcgttaatga
     3061 aaaatggatt gtaactgctg cccactgtgt tgaaactggt gttaaaatta cagtcgtcgc
     3121 aggtgaacat aatattgagg agacagaaca tacagagcaa aagcgaaatg tgattcgaat
     3181 tattcctcac cacaactaca atgcagctat taataagtac aaccatgaca ttgcccttct
     3241 ggaactggac gaacccttag tgctaaacag ctacgttaca cctatttgca ttgctgacaa
     3301 ggaatacacg aacatcttcc tcaaatttgg atctggctat gtaagtggct ggggaagagt
     3361 cttccacaaa gggagatcag ctttagttct tcagtacctt agagttccac ttgttgaccg
     3421 agccacatgt cttcgatcta caaagttcac catctataac aacatgttct gtgctggctt
     3481 ccatgaagga ggtagagatt catgtcaagg agatagtggg ggaccccatg ttactgaagt
     3541 ggaagggacc agtttcttaa ctggaattat tagctggggt gaagagtgtg caatgaaagg
     3601 caaatatgga atatatacca aggtatcccg gtatgtcaac tggattaagg aaaaaacaaa
     3661 gctcacttaa tgaaagatgg atttccaagg ttaattcatt ggaattgaaa attaacaggg
     3721 cctctcacta actaatcact gcggccgctc gagcatgcat ctagagggcc ctattctata
     3781 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     3841 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3901 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3961 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4021 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4081 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4141 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4381 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4441 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     4501 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4561 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4621 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4681 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4741 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4801 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4861 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     4921 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4981 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     5041 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     5101 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     5161 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     5221 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     5281 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     5341 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     5401 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     5461 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     5521 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     5581 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5641 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5701 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5761 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5821 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5881 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5941 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6001 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     6061 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6121 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6181 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6661 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6901 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8221 gtc
//
