LOCUS       V016455                 7781 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V016455
VERSION     V016455
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 7781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..7781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             926..3073
                     /gene="PA"
                     /label="Polymerase acidic protein"
                     /note="Polymerase acidic protein from Influenza A virus
                     (strain A/Wilson-Smith/1933 H1N1). Accession#: P15659"
     promoter        complement(3333..3351)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3377..3601
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3647..4075
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4089..4419
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4486..5277
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5454..5587
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5624..5640)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5648..5664)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5672..5702)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5717..5738)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6026..6614)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6788..7645)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7646..7750)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttaccggc
      901 cagcgaaagc aggtactgat tcaaaatgga agattttgtg cgacaatgct tcaatccgat
      961 gattgtcgag cttgcggaaa aggcaatgaa agagtatgga gaggacctga aaatcgaaac
     1021 aaacaaattt gcagcaatat gcactcactt ggaagtgtgc ttcatgtatt cagattttca
     1081 cttcatcgat gagcaaggcg agtcaatagt cgtagaactt ggcgatccaa atgcactttt
     1141 gaagcacaga tttgaaataa tcgagggaag agatcgcaca atagcctgga cagtaataaa
     1201 cagtatttgc aacactacag gggctgagaa accaaagttt ctaccagatt tgtatgatta
     1261 caagaagaat agattcatcg aaattggagt aacaaggaga gaagttcaca tatactatct
     1321 ggaaaaggcc aataaaatta aatctgagaa gacacacatc cacattttct cattcactgg
     1381 ggaggaaatg gccacaaagg ccgactacac tctcgatgaa gaaagcaggg ctaggatcaa
     1441 aaccaggcta ttcaccataa gacaagaaat ggctagcaga ggcctctggg attcctttcg
     1501 tcagtccgag agaggcgaag agacaattga agaaagattt gaaatcacag gaacaatgcg
     1561 caagcttgcc gaccaaagtc tcccgccaaa cttctccagc cttgaaaatt ttagagccta
     1621 tgtggatgga ttcgaaccga acggctacat tgagggcaag ctttctcaaa tgtccaaaga
     1681 agtaaatgct agaattgaac cttttttgaa atcaacacca cgaccactta gacttccgga
     1741 tgggcctccc tgttctcagc ggtccaaatt cctgctgatg gatgccttaa aattaagcat
     1801 tgaggaccca agtcatgagg gagaggggat accgctatat gatgcaatca aatgcatgag
     1861 aacattcttt ggatggaagg aacccaatgt tgttaaacca cacgaaaagg gaataaatcc
     1921 aaattatctt ctgtcatgga agcaagtact ggcagaactg caggacattg agaatgagga
     1981 gaaaattcca aggactaaaa atatgaagaa aacgagtcag ttaaagtggg cacttggtga
     2041 gaacatggca ccagaaaagg tagactttga cgattgtaaa gatgtaggcg atttgaagca
     2101 atatgatagt gatgaaccag aattgaggtc gcttgcaagt tggattcaga atgagttcaa
     2161 caaggcatgt gaactgaccg attcaagctg gatagagctc gatgagattg gagaagatgc
     2221 ggctccaatt gaacacattg caagcatgag aaggaattat ttcacagcag aggtgtctca
     2281 ttgcagagcc acagaataca taatgaaggg ggtgtacatc aatactgcct tgcttaatgc
     2341 atcctgtgca gcaatggatg atttccaatt gattccaatg ataagcaagt gtagaactaa
     2401 ggagggaagg cgaaagacca atttgtacgg tttcatcata aaaggaagat cccacttaag
     2461 gaatgacacc gatgtggtaa actttgtgag catggagttt tccctcactg acccaagact
     2521 tgaaccacac aaatgggaga agtactgtgt tcttgaggta ggagatatgc ttctaagaag
     2581 tgccataggc catgtgtcaa ggcctatgtt cttgtatgtg aggacaaatg gaacctcaaa
     2641 aattaaaatg aaatggggga tggaaatgag gcgttgcctc cttcagtcac ttcaacaaat
     2701 cgagagtatg attgaagctg agtcctctgt caaggagaaa gacatgacca aagagttctt
     2761 tgaaaacaaa tcagaaacat ggcccgttgg agagtccccc aaaggagtgg aggaaggttc
     2821 cattgggaag gtctgcagaa ctttattggc aaagtcggta ttcaacagct tgtatgcatc
     2881 tccacaactg gaaggatttt cagctgaatc aagaaaactg cttcttatcg ttcaggctct
     2941 tagggacaac ctggaacctg ggacctttga tcttgggggg ctatatgaag caattgagga
     3001 gtgcctgatt aatgatccct gggttttgct taatgcttct tggttcaact ccttcctcac
     3061 acatgcattg agatagttgt ggcaatgcta ctatttgcta tccatactgt ccaaaaaagt
     3121 accttgtttc tactaataac ccggcggccc aaaatgccga ctcggagcga aagatatacc
     3181 tcccccgggg ccgggaggtc gcgtcaccga ccacgccgcc ggtaagcttg gtaccgagct
     3241 cggatccact agtaacggcc gccagtgtgc tggaattctg cagatatcca tcacactggc
     3301 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
     3361 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3421 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3481 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3541 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3601 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     3661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3781 ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac
     3841 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3901 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3961 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg
     4021 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     4081 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt
     4141 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4201 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4261 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4321 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4381 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4441 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4501 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4561 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4621 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4681 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4741 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4801 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4861 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4921 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4981 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5041 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5101 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5161 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5221 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5281 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5341 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5401 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5581 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5641 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5701 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5761 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5821 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5881 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     6241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7741 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
