LOCUS       V012106                 9248 bp    DNA     circular SYN 20-DEC-2021
DEFINITION  Exported.
ACCESSION   V012106
VERSION     V012106
KEYWORDS    pcDNA3-TLR7-YFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9248)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 9248)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9248)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        120..449
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             516..1307
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1484..1617
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1654..1670)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1678..1694)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1702..1732)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1747..1768)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(1885..1902)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2056..2644)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2818..3675)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3676..3780)
                     /label="AmpR promoter"
     primer_bind     complement(3855..3874)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        4046..4425
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4426..4629
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4674..4692
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4724..7870
                     /gene="TLR7"
                     /label="Toll-like receptor 7"
                     /note="Toll-like receptor 7 from Homo sapiens. Accession#:
                     Q9NYK1"
     CDS             7877..8593
                     /label="EYFP"
                     /note="enhanced YFP"
     promoter        complement(8612..8630)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    8656..8880
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8926..9248
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
       61 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
      121 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      181 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      241 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      301 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      361 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
      421 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
      481 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
      541 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      601 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      661 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      721 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      781 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      841 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      901 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
      961 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     1021 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     1081 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1141 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1201 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1261 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     1321 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     1381 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     1441 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     1501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     1621 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1801 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1861 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2461 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2761 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2821 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2881 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     2941 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3001 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3061 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3121 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3181 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3241 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3301 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3361 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3421 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3481 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3541 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3601 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3661 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3721 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3781 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     3841 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     3901 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     3961 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     4021 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     4081 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4141 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4201 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4261 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4321 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4381 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     4441 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     4501 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     4561 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     4621 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     4681 actcactata gggagaccca agcttggtac cgagctcgga tccatggtgt ttccaatgtg
     4741 gacactgaag agacaaattc ttatcctttt taacataatc ctaatttcca aactccttgg
     4801 ggctagatgg tttcctaaaa ctctgccctg tgatgtcact ctggatgttc caaagaacca
     4861 tgtgatcgtg gactgcacag acaagcattt gacagaaatt cctggaggta ttcccacgaa
     4921 caccacgaac ctcaccctca ccattaacca cataccagac atctccccag cgtcctttca
     4981 cagactggac catctggtag agatcgattt cagatgcaac tgtgtaccta ttccactggg
     5041 gtcaaaaaac aacatgtgca tcaagaggct gcagattaaa cccagaagct ttagtggact
     5101 cacttattta aaatcccttt acctggatgg aaaccagcta ctagagatac cgcagggcct
     5161 cccgcctagc ttacagcttc tcagccttga ggccaacaac atcttttcca tcagaaaaga
     5221 gaatctaaca gaactggcca acatagaaat actctacctg ggccaaaact gttattatcg
     5281 aaatccttgt tatgtttcat attcaataga gaaagatgcc ttcctaaact tgacaaagtt
     5341 aaaagtgctc tccctgaaag ataacaatgt cacagccgtc cctactgttt tgccatctac
     5401 tttaacagaa ctatatctct acaacaacat gattgcaaaa atccaagaag atgattttaa
     5461 taacctcaac caattacaaa ttcttgacct aagtggaaat tgccctcgtt gttataatgc
     5521 cccatttcct tgtgcgccgt gtaaaaataa ttctccccta cagatccctg taaatgcttt
     5581 tgatgcgctg acagaattaa aagttttacg tctacacagt aactctcttc agcatgtgcc
     5641 cccaagatgg tttaagaaca tcaacaaact ccaggaactg gatctgtccc aaaacttctt
     5701 ggccaaagaa attggggatg ctaaatttct gcattttctc cccagcctca tccaattgga
     5761 tctgtctttc aattttgaac ttcaggtcta tcgtgcatct atgaatctat cacaagcatt
     5821 ttcttcactg aaaagcctga aaattctgcg gatcagagga tatgtcttta aagagttgaa
     5881 aagctttaac ctctcgccat tacataatct tcaaaatctt gaagttcttg atcttggcac
     5941 taactttata aaaattgcta acctcagcat gtttaaacaa tttaaaagac tgaaagtcat
     6001 agatctttca gtgaataaaa tatcaccttc aggagattca agtgaagttg gcttctgctc
     6061 aaatgccaga acttctgtag aaagttatga accccaggtc ctggaacaat tacattattt
     6121 cagatatgat aagtatgcaa ggagttgcag attcaaaaac aaagaggctt ctttcatgtc
     6181 tgttaatgaa agctgctaca agtatgggca gaccttggat ctaagtaaaa atagtatatt
     6241 ttttgtcaag tcctctgatt ttcagcatct ttctttcctc aaatgcctga atctgtcagg
     6301 aaatctcatt agccaaactc ttaatggcag tgaattccaa cctttagcag agctgagata
     6361 tttggacttc tccaacaacc ggcttgattt actccattca acagcatttg aagagcttca
     6421 caaactggaa gttctggata taagcagtaa tagccattat tttcaatcag aaggaattac
     6481 tcatatgcta aactttacca agaacctaaa ggttctgcag aaactgatga tgaacgacaa
     6541 tgacatctct tcctccacca gcaggaccat ggagagtgag tctcttagaa ctctggaatt
     6601 cagaggaaat cacttagatg ttttatggag agaaggtgat aacagatact tacaattatt
     6661 caagaatctg ctaaaattag aggaattaga catctctaaa aattccctaa gtttcttgcc
     6721 ttctggagtt tttgatggta tgcctccaaa tctaaagaat ctctctttgg ccaaaaatgg
     6781 gctcaaatct ttcagttgga agaaactcca gtgtctaaag aacctggaaa ctttggacct
     6841 cagccacaac caactgacca ctgtccctga gagattatcc aactgttcca gaagcctcaa
     6901 gaatctgatt cttaagaata atcaaatcag gagtctgacg aagtattttc tacaagatgc
     6961 cttccagttg cgatatctgg atctcagctc aaataaaatc cagatgatcc aaaagaccag
     7021 cttcccagaa aatgtcctca acaatctgaa gatgttgctt ttgcatcata atcggtttct
     7081 gtgcacctgt gatgctgtgt ggtttgtctg gtgggttaac catacggagg tgactattcc
     7141 ttacctggcc acagatgtga cttgtgtggg gccaggagca cacaagggcc aaagtgtgat
     7201 ctccctggat ctgtacacct gtgagttaga tctgactaac ctgattctgt tctcactttc
     7261 catatctgta tctctctttc tcatggtgat gatgacagca agtcacctct atttctggga
     7321 tgtgtggtat atttaccatt tctgtaaggc caagataaag gggtatcagc gtctaatatc
     7381 accagactgt tgctatgatg cttttattgt gtatgacact aaagacccag ctgtgaccga
     7441 gtgggttttg gctgagctgg tggccaaact ggaagaccca agagagaaac attttaattt
     7501 atgtctcgag gaaagggact ggttaccagg gcagccagtt ctggaaaacc tttcccagag
     7561 catacagctt agcaaaaaga cagtgtttgt gatgacagac aagtatgcaa agactgaaaa
     7621 ttttaagata gcattttact tgtcccatca gaggctcatg gatgaaaaag ttgatgtgat
     7681 tatcttgata tttcttgaga agccctttca gaagtccaag ttcctccagc tccggaaaag
     7741 gctctgtggg agttctgtcc ttgagtggcc aacaaacccg caagctcacc catacttctg
     7801 gcagtgtcta aagaacgccc tggccacaga caatcatgtg gcctatagtc aggtgttcaa
     7861 ggaaacggtc gtcgagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7921 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     7981 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     8041 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgaagtgct tcgcccgcta
     8101 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     8161 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     8221 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     8281 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     8341 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     8401 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     8461 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa
     8521 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     8581 cgagctgtac aagtaatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     8641 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     8701 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     8761 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8821 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8881 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8941 gagcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9121 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9241 ctggaaca
//
