LOCUS       40924_9851        7657 bp DNA     circular SYN 17-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pcDNA-mBeta-cat-Tcf3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Relaix F, Murphy P, Avner P, Duffy DJ.
  TITLE     MYCN Integrative Omics Reveal Context Dependent Transcriptional 
            Repressor/Activator Functions and Discover Potential Therapeutic 
            Targets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Relaix F, Murphy P, Avner P, Duffy DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2014) Systems Biology Ireland, University College 
            Dublin, Belfield, Dublin, Leinster 0004, Ireland
REFERENCE   3  (bases 1 to 7657)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7657)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2014) Systems Biology Ireland, University College Dublin, 
            Belfield, Dublin, Leinster 0004, Ireland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..7657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(827..1206)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(1391..1495)
                     /label=AmpR promoter
     enhancer        1761..2140
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2141..2344
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2389..2407
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2440..2463
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(4707..4725)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(4736..4754)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4780..5004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5050..5478
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5492..5821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     polyA_signal    6855..6988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7025..7041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7049..7065)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7073..7103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7118..7139)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(join(7427..7657,1..358))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
       61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggg
     1141 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     1201 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     1261 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     1321 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1381 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1441 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1501 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     1561 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     1621 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     1681 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     1741 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     1801 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     1861 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     1921 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     1981 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2041 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2101 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2161 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2221 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2281 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2341 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     2401 ctatagggag accctagaac tagtggatcc gccaccatgg actacaagga cgacgatgac
     2461 aaagatcccc cgatctctcg aggatccgaa ttcacatatg cagctgctgt tttgttccga
     2521 atgtctgagg acaagccaca ggattacaag aagcggcttt cagtcgagct gaccagttcc
     2581 ctcttcagga cagagccaat ggcttggaat gagactgcag atcttggact ggacattggt
     2641 gcccagggag aagcccttgg atatcgccag gatgatccca gctaccgttc ttttcactct
     2701 ggtggatacg gccaggatgc cttggggatg gaccctatga tggagcatga gatgggtggc
     2761 caccaccctg gtgctgacta tccagttgat gggctgcctg atctgggaca cgcccaggac
     2821 ctcatggatg ggctgccccc aggtgatagc aatcagctgg cctggtttga tactgacctg
     2881 cccgggatcg atgtcgacgg tatcgataag cttgataacg aatcggagaa tcagagcagt
     2941 agctcggact ccgaggcgga gaggcgcccg cagcccgccc gggacgcttt ccagaagccg
     3001 cgcgactatt tcgccgaagt gagaaggccc caggatggcg ctttttttaa gggacctgcg
     3061 tatcctgggt accccttcct gatgattcca gacctgagca gcccgtacct ctccaacgga
     3121 cccctgtctc ctggtggagc gcgcacctac ctacagatga aatggcccct cctcgatgtc
     3181 ccctccagtg ctacagtcaa ggacacaagg tcgccatctc cagcacactt gtccaacaaa
     3241 gttcctgttg ttcagcaccc tcatcacatg cacccgctga cacctctcat cacctacagc
     3301 aacgaccact tctcccctgc gtcccctccc acgcatctgt ccccagagat cgacccaaag
     3361 acaggaatcc cccggccccc tcatccatct gagctgtcac cgtattaccc actgtctcca
     3421 ggagctgtcg gacaaatccc ccatcccctc ggctggctcg tcccacagca agggcagccc
     3481 atgtactctc tccctccggg tggtttccgg catccttacc ctgccctcgc catgaacgct
     3541 tcaatgtcca gcctggtctc aagccggttc ccacacatgg tggctcctgc ccatcctggt
     3601 ctgcccacct caggaatccc ccaccctgcc atcgtctccc ccattgtgaa gcaggagcca
     3661 gcagccccca gcctgagccc tgcagtgagt gcgaaatccc cagttacggt gaagaaggaa
     3721 gaggagaaga aacctcacgt gaaaaagccc ctgaatgcct tcatgttgta tatgaaggag
     3781 atgagggcca aggtggtggc cgagtgtacc ctgaaggaaa gtgcagccat taaccaaatc
     3841 ctgggaagaa agtggcacaa cccgtcaaga gaagaacagg ccaaatacta tgagcttgcc
     3901 cggaaagaac ggcagcttca cgcgcagctc tacctaacct ggtcagcccg ggacaactat
     3961 gggaagaaga agaagaggaa gagagaaaag cagctgtcac agacacagtc tcagcagcaa
     4021 atccaagagg cagagggtgc tctggcctct aagagcaaga agccatgcat tcagtacctg
     4081 ccccctgaga agccttgtga tagccctgcg tcttcccatg gcagcacgct ggactcccct
     4141 gcgacgccct ccgcagcctt ggcctcacca gctgcccctg cggctaccca ctccgagcaa
     4201 gcgcagcccc tgtcgctcac caccaaaccg gaggcccggg cccagctggc tctccactcc
     4261 gctgccttcc tgtcggctaa ggcggccgcc agcaactcca gccagatggg cagccagccc
     4321 ccactcctat ccaggccctt gcccttgggc tctatgcccg ccgctctgct gacctctccc
     4381 cctactttcc cagctacgct ccatgcccac caggccctcc ccgtgctaca ggcccagcct
     4441 ctttccttgg tcaccaagtc tgcccactaa gctgcccccg gttctatgca ggctgtctcg
     4501 ggactccaag tagtaatgat tcagaggaga ggaaggggga aacacaaagg aagcattatt
     4561 ggtcaatatt tgaccacttt gaactgttct gtaaagtgac tggtaacagc agtgccttac
     4621 cactgtagat ataaccagta gctgatcttc aggctttttt aaaaaaaaaa aaaaaaaaaa
     4681 aagaattctg cagatatctc tagttccctt tagtgagggt taattgcggc cctattctat
     4741 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     4801 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4861 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4921 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4981 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5041 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5101 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5161 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5221 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5281 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5341 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5401 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5461 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5521 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5581 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5641 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5701 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5761 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5821 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     5881 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     5941 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6001 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6061 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6121 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6181 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6241 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6301 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6361 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6421 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6481 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6541 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatggctgac
     6601 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6661 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     6721 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6781 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6841 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6901 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6961 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7021 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7081 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7141 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7201 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7261 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7321 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7381 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7441 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7501 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7561 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7621 atagctcacg ctgtaggtat ctcagttcgg tgtaggt
//
