LOCUS       V008614                 6615 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008614
VERSION     V008614
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Zhang W, Lohman AW, Zhuravlova Y, Lu X, Wiens MD, Hoi H, Yaganoglu
            S, Mohr MA, Kitova EN, Klassen JS, Pantazis P, Thompson RJ, Campbell
            RE.
  TITLE     Optogenetic control with a photocleavable protein, PhoCl
  JOURNAL   Nat. Methods (2017) In press
   PUBMED   28288123
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Zhang W.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2017) Chemistry, University of Alberta, 11227
            Saskatchewan Drive, Department of Chemistry, CCIS 4-140, Edmonton,
            Alberta T6G2C5, Canada
REFERENCE   3  (bases 1 to 6615)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6615)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: GeneStudio v. 2.2.0.0
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Methods (2017) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-FEB-2017) Chemistry, University of Alberta, 11227 Saskatchewan
            Drive, Department of Chemistry, CCIS 4-140, Edmonton, Alberta
            T6G2C5, Canada"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             904..2181
                     /gene="PANX1"
                     /label="Pannexin-1"
                     /note="Pannexin-1 from Homo sapiens. Accession#: Q96RD7"
     polyA_signal    2215..2439
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2485..2913
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2927..3256
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3323..4114
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4291..4424
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4461..4477)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4485..4501)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4509..4539)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4554..4575)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4863..5448)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5622..6479)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6480..6584)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctagctgcc
      901 accatggcca tcgctcacct ggccacggag tacgtgttct cggatttctt gctgaaggag
      961 cccacggagc ccaagttcaa ggggctgcga ctggagctgg ctgtggacaa gatggtcacg
     1021 tgcattgcgg tggggctgcc cctgctgctc atctcgctgg ccttcgcgca ggagatctcg
     1081 attggtacac agataagctg tttctctcca agttctttct cctggcgtca ggctgccttt
     1141 gtggattcat attgctgggc ggctgttcag cagaagaact cactgcagag cgagtctgga
     1201 aacctcccac tgtggctgca taagtttttc ccctacatcc tgctgctctt tgcgatcctc
     1261 ctgtacctgc ccccgctgtt ctggcgtttc gcagctgctc ctcatatttg ctcagacttg
     1321 aagtttatca tggaagaact tgacaaagtt tacaaccgtg caattaaggc tgcaaagagt
     1381 gcgcgtgacc ttgacatgag agatggagcc tgctcagttc caggtgttac cgagaactta
     1441 gggcaaagtt tgtgggaggt atctgaaagc cacttcaagt acccaattgt ggagcagtac
     1501 ttgaagacaa agaaaaattc taataattta atcatcaagt acattagctg ccgcctgctg
     1561 acactcatca ttatactgtt agcgtgtatc tacctgggct attacttcag cctctcctca
     1621 ctctcagacg agtttgtgtg cagcatcaaa tcagggatcc tgagaaacga cagcaccgtg
     1681 cccgatcagt ttcagtgcaa actcattgcc gtgggcatct tccagttgct cagtgtcatt
     1741 aaccttgtgg tttatgtcct gctggctccc gtggttgtct acacgctgtt tgttccattc
     1801 cgacagaaga cagatgttct caaagtgtac gaaatcctcc ccacttttga tgttctgcat
     1861 ttcaaatctg aagggtacaa cgatttgagc ctctacaatc tcttcttgga ggaaaatata
     1921 agtgaggtca agtcatacaa gtgtcttaag gtactggaga atattaagag cagtggtcag
     1981 gggatcgacc caatgctact cctgacaaac cttgatgaga tggaagaatg tgcaagtcat
     2041 ttaaaaactc ccatgtctgc agagatgaga gaggagcagg ggaaccagac ggcagagctc
     2101 caaggtatga acatagacag tgaaactaaa gcaaataatg gagagaagaa tgcccgacag
     2161 cgacttctga actcttcttg ctaaaagctt ggtaaacccg ctgatcagcc tcgactgtgc
     2221 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2281 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2341 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2401 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2461 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2521 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2581 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2641 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2701 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2761 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2821 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2881 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     2941 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3001 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3061 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3121 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3181 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3241 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
     3301 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3361 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3421 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3481 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
     3541 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3601 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3661 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     3721 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     3781 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     3841 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     3901 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     3961 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4021 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4081 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4141 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4201 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4261 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     4321 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4381 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     4441 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     4501 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     4561 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     4621 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     4681 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4741 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4801 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4861 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4921 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4981 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5041 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5101 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5161 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5221 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     5281 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     5341 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     5401 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5461 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5521 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5761 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6601 gtgccacctg acgtc
//
