LOCUS       pcDNA6_myc-His_A        5126 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for expressing C-terminally Myc- and 6xHis-tagged 
            proteins. For other reading frames, use pcDNA(TM)6/myc-His B or 
            pcDNA(TM)6/myc-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA6 myc-His A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..5126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    902..999
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             997..1026
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1042..1059
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1088..1312
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1358..1786
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1800..2129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2177..2224
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2243..2638
                     /label=BSD
                     /note="blasticidin S deaminase"
     polyA_signal    2799..2932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2969..2985)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2993..3009)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3017..3047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3062..3083)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3371..3959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4133..4990)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4991..5095)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
      961 gcacagtggc ggccgctcga gtctagaggg cccttcgaac aaaaactcat ctcagaagag
     1021 gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     1081 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1141 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1201 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1261 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     1321 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     1381 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1441 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1501 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1561 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1621 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1681 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1741 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     1801 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1861 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1921 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     1981 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2041 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     2101 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     2161 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     2221 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
     2281 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     2341 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     2401 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     2461 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     2521 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     2581 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     2641 agcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     2701 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     2761 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     2821 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2881 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     2941 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3001 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3061 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3121 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3181 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3241 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3301 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3361 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3421 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3481 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3541 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3601 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3661 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3721 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3781 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3841 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3901 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3961 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4021 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4081 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4141 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4201 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4261 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4321 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4381 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4441 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4501 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4561 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4621 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4681 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4741 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4801 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4861 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4921 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4981 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5041 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5101 ttccccgaaa agtgccacct gacgtc
//
