LOCUS       pcDNA4_V5-His_B.        5096 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for expressing C-terminally V5- and 6xHis-tagged 
            proteins. For other reading frames, use pcDNA(TM)4/V5-His A or 
            pcDNA(TM)4/V5-His C.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA4 V5-His B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The cloned gene must include a Kozak sequence and start codon.
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    895..1012
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1013..1054
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1064..1081
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1110..1334
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1380..1808
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1822..2151
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2199..2246
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2265..2636
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2769..2902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2939..2955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2963..2979)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2987..3017)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3032..3053)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3341..3929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4103..4960)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4961..5065)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     1021 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     1081 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1141 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1201 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1261 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     1321 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     1381 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1441 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1501 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1561 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1621 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1681 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1741 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1801 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     1861 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     1921 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     1981 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2041 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     2101 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     2161 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     2221 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     2281 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     2341 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     2401 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     2461 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     2521 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     2581 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     2641 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     2701 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     2761 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2821 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2881 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     2941 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3001 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3061 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3121 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
