LOCUS       V008976                 4942 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008976
VERSION     V008976
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Gourguechon S, Cande WZ.
  TITLE     Rapid tagging and integration of genes in Giardia intestinalis
  JOURNAL   Eukaryotic Cell (2010) In press
   PUBMED   21115739
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Gourguechon S, Cande WZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2010) Mol and Cell Biology, University of
            California, Berkeley, 345 LSA, Berkeley, CA 94720-3200, USA
REFERENCE   3  (bases 1 to 4942)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell (2010) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-NOV-2010) Mol and Cell Biology, University of California,
            Berkeley, 345 LSA, Berkeley, CA 94720-3200, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1687..1713
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1717..1743
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1747..1773
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2184..2603
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(2754..2772)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2793..2809)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2817..2833)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2841..2871)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2886..2907)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3195..3783)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3957..4814)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4815..4919)
                     /label="AmpR promoter"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggtaccat
      661 tccacaattg acggcaatcg ctacggctat agcccgaggg gcgcctctaa cgatcgttta
      721 cctgagcgcc gacgctcgaa aaggggtgag gattatcgga aagaacacac ttacaaaccc
      781 accatttcca agtttgcgca ggcgctgcct cgctcagatg ttccgtgtca cgaacggctg
      841 tattcagcca gacctagtgt tcaacggaac aagaatgcag atggtgagga ctacgaggca
      901 ccggcactct ctccgcgttt gcaggagttt gtgaacaaca gcagttttga gttgttccgt
      961 ccccacatat caaagcgtgc aagggcttta gaggtcgaag cgcctttcta cgatcgtctc
     1021 catccatcca aggcctctaa cagacttgtt ggcatttgct gtcttttcat tgcagcaaag
     1081 tacgagcaaa gctatattcc ttcacttagg cattttgtgg aggcgctgac ccaggtcgac
     1141 ccggaattcc tcgtcaattg taagtgcgag ggtaaccgtt cgggaccgtg ctacaggtgc
     1201 agtcgcgaag ctataataga tacggaagaa tctatcctgc atacgctgga tttccagctg
     1261 gctatgccca caattctcac attcctacga aggtatgcca gaatttcgga aatgaagaat
     1321 cgagaccgtt acattgcatt ttatatctct gaactctcgt gtctgacgac aagtatcttc
     1381 aaatttaagc ctagcgagat tgcggcaggc tgcattgcga tgtcccgtag attgactcgg
     1441 aagccgactt gggacgaaac cctcgaaaag tattctggat acactgagag cgatacaaaa
     1501 aaggttatgg cggagatttc tacgatcatg aagaaatacg cttgtaaccc gaaggccaga
     1561 tacataaggc gcaaatactc gcaagaggat cgcttctacg gggtgtcctt gatgattgag
     1621 actgtggtaa cgcaggcgct aactcttaat acaaaggaag caaagcccgg gctcgagctt
     1681 aaggaatatc cttatgacgt cccagactac gcggagtacc catacgatgt acctgattat
     1741 gctgagtacc catacgacgt accagattac gctggatcct gaatcgaatt cctttgcatc
     1801 atcgttgctc ggtcgtctgc ctcgtttacg acctgcttgt tcctgggcct caaatggtta
     1861 gtggttttcc ttcttccaac ggcgttggtc catcctcggc cgcgtgatca aatgccttcg
     1921 agcgtatgtc tgacctgttt gtgaatgacg atccgtggct ttactcagat tcctgtgttt
     1981 gtgaataaga aatccgtgaa tttgttgggg tggaacggcg aagtggcctc tgctacaata
     2041 agaactggag agtgtgttca gaggcgaatg cgcaacgaac tttgtcgtta cacgacgtgt
     2101 gcgcctgtgg tcttcgtgcg gtttccgcgc ggaattttgc gccttttcta aaaaactaac
     2161 cctaaaacaa gaaaatcatg ggaatgaaaa catttaacat ttctcaacaa gatctagaat
     2221 tagtagaagt agcgacagag aagattacaa tgctttatga ggataataaa catcatgtgg
     2281 gagcggcaat tcgtacgaaa acaggagaaa tcatttcggc agtacatatt gaagcgtata
     2341 taggacgagt aactgtttgt gcagaagcca ttgcgattgg tagtgcagtt tcgaatggac
     2401 aaaaggattt tgacacgatt gtagctgtta gacaccctta ttctgacgaa gtagatagaa
     2461 gtattcgagt ggtaagtcct tgtggtatgt gtagggagtt gatttcagac tatgcaccag
     2521 attgttttgt gttaatagaa atgaatggca agttagtcaa aactacgatt gaagaactca
     2581 ttccactcaa atatacccga aatgtcgagt gagggctgaa gtgaatattt accttttccg
     2641 ggttcgacgg gcgtgcttga cgatgcggaa gtcgctgtcc gttccaacgg ctatctgccc
     2701 caggaccccc atcccggcgc cgatcagctg gtaccgagct ccagcttttg ttccctttag
     2761 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2821 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2881 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2941 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3001 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3061 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3121 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3181 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3241 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3301 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3361 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3421 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3481 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3541 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3601 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3661 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3721 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3781 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3841 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3901 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3961 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4021 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4081 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4141 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4201 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4261 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4321 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4381 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4441 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4501 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4561 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4621 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4681 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4741 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4801 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4861 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4921 acatttcccc gaaaagtgcc ac
//
