LOCUS       V008978                 4663 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008978
VERSION     V008978
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Gourguechon S, Cande WZ.
  TITLE     Rapid tagging and integration of genes in Giardia intestinalis
  JOURNAL   Eukaryotic Cell (2010) In press
   PUBMED   21115739
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Gourguechon S, Cande WZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2010) Mol and Cell Biology, University of
            California, Berkeley, 345 LSA, Berkeley, CA 94720-3200, USA
REFERENCE   3  (bases 1 to 4663)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell (2010) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-NOV-2010) Mol and Cell Biology, University of California,
            Berkeley, 345 LSA, Berkeley, CA 94720-3200, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             653..1498
                     /codon_start=1
                     /product="tagged cyclin-dependent kinase 1"
                     /label="tagged cyclin-dependent kinase 1"
                     /note="Cdk1"
                     /protein_id="ADR65092.1"
                     /translation="ELMSVIHHNHRLHLIFEYAENDLKKYMDKNPDVSMRVIKSFLYQL
                     INGVNFCHSRRCLHRDLKPQNLLLSVSDASETPVLKIGDFGLARAFGIPIRQFTHEIIT
                     LWYRPPEILLGSRHYSTSVDIWSIACIWAEMLMKTPLFPGDSEIDQLFKIFEVLGLPDD
                     TTWPGVTALPDWKQSFPKFRGKTLKRVLGALLDDEGLDLLTAMLEMDPVKRISAKNALE
                     HPYFSHNDFDPPGVELKRSEEQKLISEEDLLRSEEQKLISEEDLLRSEEQKLISEEDLL
                     "
     misc_feature    1373..1498
                     /label="3Myc epitope tag"
                     /note="3Myc epitope tag"
     CDS             1379..1408
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             1421..1450
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             1463..1492
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             1908..2327
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(2475..2493)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2514..2530)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2538..2554)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2562..2592)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2607..2628)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2916..3504)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3678..4535)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4536..4640)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcat
      661 gtccgttatc catcacaatc accgtctcca tctgatcttc gagtatgccg agaacgacct
      721 gaagaagtac atggataaga accctgatgt aagcatgcgc gtgatcaagt cattcttgta
      781 tcagctcatc aatggagtca acttctgcca cagcagacgc tgcctccacc gagacctgaa
      841 acctcagaat ctgctcctca gcgtcagcga tgcgtccgag acccctgtct tgaagatagg
      901 ggactttggc ctggcgagag cgttcggcat tcccattcgt cagttcacac acgagataat
      961 cacactctgg tacaggccgc ccgagatcct ccttggcagc agacactaca gcacgtctgt
     1021 cgacatttgg tccattgcat gcatctgggc cgagatgctg atgaagacac ccctcttccc
     1081 cggcgacagt gagattgacc agcttttcaa gatatttgag gtactcgggc tccccgacga
     1141 cacaacgtgg cctggggtta cggccctccc agattggaag cagagcttcc cgaagttccg
     1201 ggggaagact ctcaagagag ttttgggggc tcttcttgac gatgaaggac tcgacctcct
     1261 cactgccatg ctcgagatgg atcccgtcaa gcgcattagc gcgaagaacg ctctcgagca
     1321 cccgtacttc tcccacaacg attttgaccc tcccggggtc gagcttaaga gatccgagga
     1381 gcagaagctg atctcggagg aggatctgct gcgtagtgaa gaacaaaaac ttataagtga
     1441 agaagattta ttgaggtcag aggaacagaa attaatttca gaagaggacc ttttatgagg
     1501 atcctgaatc gaattctttg catcatcgtt gctcggtcgt ctgcctcgtt tacgacctgc
     1561 ttgttcctgg gcctcaaatg gttagtggtt ttccttcttc caacggcgtt ggtccatcct
     1621 cggccgcgtg atcaaatgcc ttcgagcgta tgtctgacct gtttgtgaat gacgatccgt
     1681 ggctttactc agattcctgt gtttgtgaat aagaaatccg tgaatttgtt ggggtggaac
     1741 ggcgaagtgg cctctgctac aataagaact ggagagtgtg ttcagaggcg aatgcgcaac
     1801 gaactttgtc gttacacgac gtgtgcgcct gtggtcttcg tgcggtttcc gcgcggaatt
     1861 ttgcgccttt tctaaaaaac taaccctaaa acaagaaaat catgggaatg aaaacattta
     1921 acatttctca acaagatcta gaattagtag aagtagcgac agagaagatt acaatgcttt
     1981 atgaggataa taaacatcat gtgggagcgg caattcgtac gaaaacagga gaaatcattt
     2041 cggcagtaca tattgaagcg tatataggac gagtaactgt ttgtgcagaa gccattgcga
     2101 ttggtagtgc agtttcgaat ggacaaaagg attttgacac gattgtagct gttagacacc
     2161 cttattctga cgaagtagat agaagtattc gagtggtaag tccttgtggt atgtgtaggg
     2221 agttgatttc agactatgca ccagattgtt ttgtgttaat agaaatgaat ggcaagttag
     2281 tcaaaactac gattgaagaa ctcattccac tcaaatatac ccgaaattaa gtcgagtgag
     2341 ggctgaagtg aatatttacc ttttccgggt tcgacgggcg tgcttgacga tgcggaagtc
     2401 gctgtccgtt ccaacggcta tctgccccag gacccccatc ccggcgccga tcagctggta
     2461 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     2521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2581 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2641 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2701 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     3361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4561 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4621 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
