LOCUS       V009159                 4094 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009159
VERSION     V009159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Liu TZ.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2013) Guangzhou Institutes of Biomedicine and
            Health, Chinese Academy of Sciences, 190 Kaiyuan Road, Science Park,
            Guangdong, Guangzhou 510530, China
REFERENCE   2  (bases 1 to 4094)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4094)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (06-OCT-2013) Guangzhou Institutes of Biomedicine and Health,
            Chinese Academy of Sciences, 190 Kaiyuan Road, Science Park,
            Guangdong, Guangzhou 510530, China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2226..2242
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      2272..2321
                     /label="artifical promoter"
                     /note="artifical promoter"
                     /regulatory_class="promoter"
     misc_feature    2277..2304
                     /label="MTB dif"
                     /note="MTB dif"
     CDS             2366..3361
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    3377..3404
                     /label="MTB dif"
                     /note="MTB dif"
     promoter        complement(3451..3469)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3479..3495)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3637..4092
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctctag aactagtgga tccgatatcg aattccatat gcccaagctt ctcgagactt
     2281 gacataatgt cgcttatcgg cttaatcgat ctagaccggc cgtgcggaat taagccggcc
     2341 cgtaccctgt gaatagaggt ccgctgtgac acaagaatcc ctgttacttc tcgaccgtat
     2401 tgattcggat gattcctacg cgagcctgcg gaacgaccag gagttctggg agccgctggc
     2461 ccgccgagcc ctggaggagc tcgggctgcc ggtgccgccg gtgctgcggg tgcccggcga
     2521 gagcaccaac cccgtactgg tcggcgagcc cggcccggtg atcaagctgt tcggcgagca
     2581 ctggtgcggt ccggagagcc tcgcgtcgga gtcggaggcg tacgcggtcc tggcggacgc
     2641 cccggttccg gtgccccgcc tcctcggccg cggcgagctg cggcccggca ccggagcctg
     2701 gccgtggccc tacctggtga tgagccggat gaccggcacc acctggcggt ccgcgatgga
     2761 cggcacgacc gaccggaacg cgctgctcgc cctggcccgc gaactcggcc gggtgctcgg
     2821 acggctgcac agggtgccgc tgaccgggaa caccgtgctc accccccatt ccgaggtctt
     2881 cccggaactg ctgcgggaac gccgcgcggc gaccgtcgag gaccaccgcg ggtggggcta
     2941 cctctcgccc cggctgctgg accgcctgga ggactggctg ccggacgtgg acacgctgct
     3001 ggccggccgc gaaccccggt tcgtccacgg cgacctgcac gggaccaaca tcttcgtgga
     3061 cctggccgcg accgaggtca ccgggatcgt cgacttcacc gacgtctatg cgggagactc
     3121 ccgctacagc ctggtgcaac tgcatctcaa cgccttccgg ggcgaccgcg agatcctggc
     3181 cgcgctgctc gacggggcgc agtggaagcg gaccgaggac ttcgcccgcg aactgctcgc
     3241 cttcaccttc ctgcacgact tcgaggtgtt cgaggagacc ccgctggatc tctccggctt
     3301 caccgatccg gaggaactgg cgcagttcct ctgggggccg ccggacaccg cccccggcgc
     3361 ctgatctaga cccgggactt gacataatgt cgcttatcgg cttactcgag aagcttgcca
     3421 tggctgcagc gctagcggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     3481 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3541 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3601 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     3661 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3721 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3781 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3841 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3901 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3961 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4021 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4081 cgcttacaat ttag
//
