LOCUS       40924_5059        3670 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pattPGGsaacoriT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
  PUBMED    24473925
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10"; 
            pages: "4557-4570"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     protein_bind    409..442
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     RBS             542..550
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             556..1329
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     misc_feature    1342..1583
                     /label=oriT
                     /note="oriT"
     misc_recomb     1609..1658
                     /label=P-GG
                     /note="P-GG"
     primer_bind     complement(1665..1684)
     rep_origin      complement(1923..2511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2685..3542)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3543..3647)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccat aacttcgtat
      421 aatgtatgct atacgaagtt atatttaaat tacaaatggc cgttgacagc ccaagcaaat
      481 actcatataa tcatagaaaa gcggctatat cgctacagct tccagtagga tacaagaagt
      541 ttaaggaggt aacaaatgca gtacgagtgg cgcaaggcgg agctgatcgg ccagctcctg
      601 aacctgggtg tcacgccggg tggcgtgctg ctggtgcaca gcagcttccg cagcgtccgc
      661 ccgctggagg acggtccgct gggcctgatc gaggccctgc gcgcggcgct gggtccgggt
      721 ggcacgctgg tgatgccgag ctggtccggc ctggacgacg aaccgttcga cccggccacc
      781 agcccggtca ccccggacct gggcgtggtg agcgacacgt tctggcgcct gccgaacgtc
      841 aagcgcagcg cgcacccgtt cgcgttcgcc gccgccggtc cgcaggcgga gcagatcatc
      901 tccgacccgc tgccgctgcc gccgcacagc ccggcgtccc cggtcgcgcg cgtccacgag
      961 ctggacggtc aggtgctgct gctgggcgtc ggccacgacg ccaacaccac cctgcacctg
     1021 gccgagctga tggccaaggt cccgtacggc gtcccgcgcc attgcaccat ccttcaggac
     1081 ggcaagctgg tgcgcgtgga ctacctggag aacgaccact gctgcgagcg cttcgcgctg
     1141 gcggaccgct ggctgaagga aaagagcctc cagaaggaag gcccggtggg ccacgccttc
     1201 gcccgcctga tccgctcccg cgacatcgtc gcgaccgccc tgggtcaact gggtcgcgac
     1261 ccgctgatct tcctgcatcc gccggaagcc ggctgcgaag agtgcgacgc ggcgcgccag
     1321 tccatcggct gataagatat ccgccagcct cgcagagcag gattcccgtt gagcaccgcc
     1381 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
     1441 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
     1501 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
     1561 aagcagggtt atgcagcgga aaagatatca tttgtatcta gagatatcgt agtgccccaa
     1621 ctggggtaac ctgggagttc tctcagttgg gggcgtagtc tagacgaagc agctccagcc
     1681 tacacagctg attaattaac tggcctcatg ggccttccgc tcactgcccg ctttccagtc
     1741 gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc
     1801 gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt
     1861 gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     2101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     2161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2341 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     2401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2821 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3601 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3661 aaagtgccac
//
