LOCUS       V009399                 3907 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009399
VERSION     V009399
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
   PUBMED   24473925
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10";
            pages: "4557-4570"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label="B-CC"
                     /note="B-CC"
     protein_bind    472..505
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     RBS             620..628
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             634..1629
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1642..1883
                     /label="oriT"
                     /note="oriT"
     primer_bind     complement(1902..1921)
     rep_origin      complement(2160..2748)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2922..3779)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3780..3884)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccgg agtacgcgcc
      421 cggggagccc gggggcacgc cctggcaccc gcaccgcggg atatcggtac cataacttcg
      481 tataatgtat gctatacgaa gttatattta aattacaaat aatacttgac atatcactgt
      541 gattcacata taatatgcgc aggtcggctg gttggctgac aaagtgcgta ggatgactgt
      601 actacaatca aaaaaataat aaggaggtaa atcatgaccc aggagtccct gctgctgctg
      661 gaccgcatcg acagcgacga cagctacgcg agcctgcgca acgaccagga gttctgggag
      721 ccgctcgccc gccgcgcgct ggaagagctg ggcctgccgg tcccgccggt gctgcgcgtg
      781 ccgggtgagt ccaccaaccc ggtgctggtg ggcgagccgg acccggtcat caagctgttc
      841 ggggagcact ggtgcggccc ggagagcctg gcgagcgaga gcgaggcgta cgcggtgctg
      901 gccgacgccc cggtcccggt gccgcgcctg ctgggtcgcg gcgagctgcg cccaggcacg
      961 ggtgcctggc cgtggccgta cctggtgatg tcccgcatga cgggcaccac gtggcgcagc
     1021 gcgatggacg gcaccaccga ccgcaacgcc ctgctggccc tggcgcgcga actgggtcgc
     1081 gtcctgggtc gcctgcatcg cgtcccgctg accggcaaca cggtgctgac cccgcactcc
     1141 gaagtgttcc cggagctgct gcgcgagcgc cgcgccgcca ccgtcgagga ccaccggggc
     1201 tggggctacc tgagcccgcg cctgctggac cgcctggaag actggctgcc ggacgtcgac
     1261 acgctgctgg cgggtcgcga gccgcgcttc gtgcacggcg acctgcacgg caccaacatc
     1321 ttcgtcgacc tggcggccac cgaggtcacc ggcatcgtcg acttcacgga cgtgtacgcc
     1381 ggtgactccc gctactccct ggtccagctt catctgaacg ccttccgcgg tgaccgcgag
     1441 atcctggcgg cgctgctgga cggtgcgcag tggaagcgca ccgaggactt cgcgcgcgag
     1501 ctgctggcct tcacgttcct ccacgacttc gaggtcttcg aagaaacccc gctggacctg
     1561 agcggcttca ccgacccgga agaactggcg cagttcctgt ggggtccgcc ggacacggcg
     1621 ccgggcgcgt gataagatat ccgccagcct cgcagagcag gattcccgtt gagcaccgcc
     1681 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
     1741 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
     1801 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
     1861 aagcagggtt atgcagcgga aaagatatcc ccgtatctag acgaagcagc tccagcctac
     1921 acagctgatt aattaactgg cctcatgggc cttccgctca ctgcccgctt tccagtcggg
     1981 aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct
     2041 ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc
     2101 taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3061 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3901 gtgccac
//
