LOCUS       V009401                10124 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009401
VERSION     V009401
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
  TITLE     The Q system: a repressible binary system for transgene expression,
            lineage tracing, and mosaic analysis
  JOURNAL   Cell 141 (3), 536-548 (2010)
   PUBMED   20434990
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   Potter CJ, Tasic B, Luo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2010) Neuroscience, The Johns Hopkins School of
            Medicine, 855 N. Wolfe Street, Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 10124)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell";
            date: "2010"; volume: "141"; issue: "3"; pages: "536-548"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-APR-2010) Neuroscience, The Johns Hopkins School of Medicine,
            855 N. Wolfe Street, Baltimore, MD 21205, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label="mini-white"
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4874..7321
                     /gene="qa-1f"
                     /label="Transcription factor qa-1f"
                     /note="Transcription factor qa-1f from Neurospora crassa
                     (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
                     FGSC 987). Accession#: P11638"
     regulatory      7331..7587
                     /label="hsp70 terminator"
                     /note="hsp70 terminator"
                     /regulatory_class="terminator"
     protein_bind    7648..7717
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7936..7954)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7975..7991)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7999..8015
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8023..8053)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8068..8089)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8377..8965)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9139..9996)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9997..10101)
                     /label="AmpR promoter"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgggaat
     4861 tgggaattcc aacatgccgc ctaaacgcaa gacactcaat gccgctgccg aagccaatgc
     4921 ccacgctgat ggccatgctg atggcaatgc tgatggtcat gtcgctaaca ctgcagcaag
     4981 cagcaacaac gcccgttttg cggacttgac caacattgac acacccggcc tcggccctac
     5041 cacgacgacg ttacttgtcg agcccgctcg ttcgaaacgc cagagagtct cgagggcctg
     5101 tgatcagtgt cgagctgcac gtgaaaagtg tgatggaatc cagccggctt gcttcccctg
     5161 tgtgtcgcag ggccggtcgt gtacctacca ggccagtccc aagaagcgag gagtccagac
     5221 gggctacatc cgcactctcg aactggctct ggcttggatg ttcgagaacg ttgcccgcag
     5281 cgaggacgcc ctccacaatc ttttggtccg tgatgctggc cagggcagcg ctctcctggt
     5341 cggcaaagac tcgcctgctg cagaacgcct gcatgcaaga tgggcgacga gtcgagtcaa
     5401 caaaagcatc acccgtcttc tctcaggtca ggccgcacaa gatccatctg aagacggcca
     5461 atccccgtcc gaagacataa atgtccaaga tgccggggca aagacatccg acttccctca
     5521 tgcgcctcac ttgactttct cggcgcctaa gtctagtaca gctgagacac gcactctacc
     5581 aggcccggtc cgaccaccta tttcggcaaa caccctggaa aacaaccttc agccagatgg
     5641 taccgggata gggaagctac cacccaatca ttggcgcctg ctggatatct acttttccta
     5701 cacgcattct tggctcccta tcctcgagaa gaaagacatg taccaagcat tgtaccagta
     5761 ctctgaacaa ggctcgttgc ttccctctgc gaatgtcgag tctggcgttc atgccgagct
     5821 ctggagcgcg ctcgccctgg cgtccttcca ggctgctgct actgctgcat cgagtgctac
     5881 gggtccagct tcagctgctc atggccatga caatgccatc aatccttcac ctgcagacat
     5941 atctgacaca gcccgaaagc tcataccttt ggaaagcggg ccgttccagg ttcagcactg
     6001 cagagcgttg ctgcttcttt gtctcgtaag ccttgggcgg gatgattggg agtctgcttg
     6061 gttgctggtt ggctttgcgg tccgcgtcct acttgttgtt cgcacccagt tgcctcctga
     6121 tgatgaccgg ccacgaccaa gaatgcgtgc gctgctcgtc gcgtgcttca tcgtggatac
     6181 cattgtgtct atgagacaca acgtgccggc ccatctcaag ccagacgaca ttgcggatct
     6241 gccgttacct gaagacggtc aagatcaatg ggagccgtgg acaccatgtg agggcttagg
     6301 cggtgaacac accatgctgc aaatgttgag gaacccggca taccctttaa gcacattcaa
     6361 ccacctatat ggcgtgacca agctggttgc tttggagctt ctgccaagaa tacgaacatc
     6421 ttcacagaac gctcccttgg agttcaggtc gcggttgcag caggtaatcg gccacaattc
     6481 tcccttcagc gtctttgtcc tttcccagga tacagcatcg gcttttgtgc ctactgcata
     6541 ccttacccgt accgtttatt tatgggcagc tgccttttct gagcctctca acgaacacta
     6601 ctcgcatctt ctgatcgaaa ctcttgatca gtatcagaag cggtttggta catatgcaat
     6661 cccacctctg atcccttctc ttctagactc ccttcttgct ttaaagaaac aatcacattc
     6721 ttcagagcgg catcgaaggc acttggaaga gcttttcccc gcctactcct ccatttggcc
     6781 tcggggaggc cgacacagca atactggcct ccaacccata cgacaacttg agcttccgcc
     6841 gactgcgact gccactgcaa gtatcatgcc ccatgtcatg gaacagcccc tgtcaacgtc
     6901 aataaatccg gtcaatgatc ggtttaatgg aataccgaat cctaccccct acaatagcga
     6961 tgcggcccta gacgcaatta ctcaaaccaa tgactacgga tcagtcaata cccatggcat
     7021 tctcagtaca tatccaccac ctgccaccca cttgaacgag gcctcggtgg cccttgcgcc
     7081 tggaggtgct cctcccagac cacccccgcc atacgttgat agtacaacga accatcctcc
     7141 ttaccatagt aacctcgtcc ccatggccaa tttcggatat tcgactgtag actatgatgc
     7201 gatggtggat gatctggcat cgatcgagta cacggatgcg gttgacgttg atccgcagtt
     7261 catgacgaac ctcgggtttg tcccagggtg taattttagc gatatcaaca cgtatgagca
     7321 ataggacgtc tatcgatacc gtcgactaaa gccaaataga aaattattca gttcctggct
     7381 taagttttta aaagtgatat tatttatttg gttgtaacca accaaaagaa tgtaaataac
     7441 taatacataa ttatgttagt tttaagttag caacaaattg attttagcta tattagctac
     7501 ttggttaata aatagaatat atttatttaa agataattgc gtttttattg tcagggagtg
     7561 agtttgctta aaaactcgtt tagatccgcg gccgcggctc gagggtacct ctagagatcc
     7621 actagtgtcg acgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc agggcgtgcc
     7681 cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc
     7741 gaggtggcgg tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa
     7801 accgctgggc gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga
     7861 tacgcggggc agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagttct
     7921 agccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     7981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     8821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
