LOCUS       V009402                 3649 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009402
VERSION     V009402
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
   PUBMED   24473925
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3649)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3649)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10";
            pages: "4557-4570"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label="B-CC"
                     /note="B-CC"
     RBS             566..574
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             580..1575
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_recomb     1588..1637
                     /label="P-GG"
                     /note="P-GG"
     primer_bind     complement(1644..1663)
     rep_origin      complement(1902..2490)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2664..3521)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3522..3626)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccgg agtacgcgcc
      421 cggggagccc gggggcacgc cctggcaccc gcaccgcggg ataaataata cttgacatat
      481 cactgtgatt cacatataat atgcgcaggt cggctggttg gctgacaaag tgcgtaggat
      541 gactgtacta caatcaaaaa aataataagg aggtaaatca tgacccagga gtccctgctg
      601 ctgctggacc gcatcgacag cgacgacagc tacgcgagcc tgcgcaacga ccaggagttc
      661 tgggagccgc tcgcccgccg cgcgctggaa gagctgggcc tgccggtccc gccggtgctg
      721 cgcgtgccgg gtgagtccac caacccggtg ctggtgggcg agccggaccc ggtcatcaag
      781 ctgttcgggg agcactggtg cggcccggag agcctggcga gcgagagcga ggcgtacgcg
      841 gtgctggccg acgccccggt cccggtgccg cgcctgctgg gtcgcggcga gctgcgccca
      901 ggcacgggtg cctggccgtg gccgtacctg gtgatgtccc gcatgacggg caccacgtgg
      961 cgcagcgcga tggacggcac caccgaccgc aacgccctgc tggccctggc gcgcgaactg
     1021 ggtcgcgtcc tgggtcgcct gcatcgcgtc ccgctgaccg gcaacacggt gctgaccccg
     1081 cactccgaag tgttcccgga gctgctgcgc gagcgccgcg ccgccaccgt cgaggaccac
     1141 cggggctggg gctacctgag cccgcgcctg ctggaccgcc tggaagactg gctgccggac
     1201 gtcgacacgc tgctggcggg tcgcgagccg cgcttcgtgc acggcgacct gcacggcacc
     1261 aacatcttcg tcgacctggc ggccaccgag gtcaccggca tcgtcgactt cacggacgtg
     1321 tacgccggtg actcccgcta ctccctggtc cagcttcatc tgaacgcctt ccgcggtgac
     1381 cgcgagatcc tggcggcgct gctggacggt gcgcagtgga agcgcaccga ggacttcgcg
     1441 cgcgagctgc tggccttcac gttcctccac gacttcgagg tcttcgaaga aaccccgctg
     1501 gacctgagcg gcttcaccga cccggaagaa ctggcgcagt tcctgtgggg tccgccggac
     1561 acggcgccgg gcgcgtgata agatatcgta gtgccccaac tggggtaacc tgggagttct
     1621 ctcagttggg ggcgtagtct agacgaagca gctccagcct acacagctga ttaattaact
     1681 ggcctcatgg gccttccgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1741 gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac
     1801 tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag
     1861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2761 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     2821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
