LOCUS       V009403                 3903 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009403
VERSION     V009403
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
   PUBMED   24473925
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3903)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3903)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10";
            pages: "4557-4570"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes,
            Saarbruecken, Saarland 66123, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label="B-CC"
                     /note="B-CC"
     RBS             566..574
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             580..1575
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     misc_feature    1588..1829
                     /label="oriT"
                     /note="oriT"
     misc_recomb     1842..1891
                     /label="P-GG"
                     /note="P-GG"
     primer_bind     complement(1898..1917)
     rep_origin      complement(2156..2744)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2918..3775)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3776..3880)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccgg agtacgcgcc
      421 cggggagccc gggggcacgc cctggcaccc gcaccgcggg ataaataata cttgacatat
      481 cactgtgatt cacatataat atgcgcaggt cggctggttg gctgacaaag tgcgtaggat
      541 gactgtacta caatcaaaaa aataataagg aggtaaatca tgacccagga gtccctgctg
      601 ctgctggacc gcatcgacag cgacgacagc tacgcgagcc tgcgcaacga ccaggagttc
      661 tgggagccgc tcgcccgccg cgcgctggaa gagctgggcc tgccggtccc gccggtgctg
      721 cgcgtgccgg gtgagtccac caacccggtg ctggtgggcg agccggaccc ggtcatcaag
      781 ctgttcgggg agcactggtg cggcccggag agcctggcga gcgagagcga ggcgtacgcg
      841 gtgctggccg acgccccggt cccggtgccg cgcctgctgg gtcgcggcga gctgcgccca
      901 ggcacgggtg cctggccgtg gccgtacctg gtgatgtccc gcatgacggg caccacgtgg
      961 cgcagcgcga tggacggcac caccgaccgc aacgccctgc tggccctggc gcgcgaactg
     1021 ggtcgcgtcc tgggtcgcct gcatcgcgtc ccgctgaccg gcaacacggt gctgaccccg
     1081 cactccgaag tgttcccgga gctgctgcgc gagcgccgcg ccgccaccgt cgaggaccac
     1141 cggggctggg gctacctgag cccgcgcctg ctggaccgcc tggaagactg gctgccggac
     1201 gtcgacacgc tgctggcggg tcgcgagccg cgcttcgtgc acggcgacct gcacggcacc
     1261 aacatcttcg tcgacctggc ggccaccgag gtcaccggca tcgtcgactt cacggacgtg
     1321 tacgccggtg actcccgcta ctccctggtc cagcttcatc tgaacgcctt ccgcggtgac
     1381 cgcgagatcc tggcggcgct gctggacggt gcgcagtgga agcgcaccga ggacttcgcg
     1441 cgcgagctgc tggccttcac gttcctccac gacttcgagg tcttcgaaga aaccccgctg
     1501 gacctgagcg gcttcaccga cccggaagaa ctggcgcagt tcctgtgggg tccgccggac
     1561 acggcgccgg gcgcgtgata agatatccgc cagcctcgca gagcaggatt cccgttgagc
     1621 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     1681 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     1741 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     1801 accccgaagc agggttatgc agcggaaaag atatccccat cgtagtgccc caactggggt
     1861 aacctgggag ttctctcagt tgggggcgta gtctagacga agcagctcca gcctacacag
     1921 ctgattaatt aactggcctc atgggccttc cgctcactgc ccgctttcca gtcgggaaac
     1981 ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg ggcgctctcc
     2041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg ggtgcctaat
     2101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2581 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2641 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2701 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2761 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2821 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2881 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2941 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3001 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
     3061 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3121 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3181 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3241 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3301 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3361 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3421 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3481 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3541 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3601 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3661 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3721 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3901 cac
//
