LOCUS       40924_5014        3698 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector patt-saadA-oriT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
  PUBMED    24473925
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3698)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3698)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10"; 
            pages: "4557-4570"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label=B-CC
                     /note="B-CC"
     CDS             581..1369
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    1382..1623
                     /label=oriT
                     /note="oriT"
     misc_recomb     1637..1686
                     /label=P-GG
                     /note="P-GG"
     primer_bind     complement(1693..1712)
     rep_origin      complement(1951..2539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2713..3570)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3571..3675)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccgg agtacgcgcc
      421 cggggagccc gggggcacgc cctggcaccc gcaccgcggg ataaatacct attgacaatt
      481 aaaggctaaa atgctataat tccaccacgc tggctacatt atacctcggc atagtaggat
      541 atccgccaca ttaaacaatc agataaagag gaggtttccc atgcgcgagg ccgtgatcgc
      601 cgaggtgtcc acgcagctca gcgaggtggt cggcgtcatc gagcgccacc tggagccgac
      661 cctgctggcc gtccacctgt acggctccgc cgtggacggt ggcctgaagc cgcacagcga
      721 catcgacctg ctggtgaccg tcaccgtccg cctggacgaa accacccgcc gcgcgctgat
      781 caacgacctg ctggaaacca gcgcgtcccc aggtgagagc gagatcctgc gcgcggtcga
      841 ggtcaccatc gtggtgcacg acgacatcat cccgtggcgc tacccggcca agcgcgagtt
      901 gcagttcggc gaatggcagc gcaacgacat cctggcgggt atcttcgagc cggcgacgat
      961 cgacatcgac ctggcgatcc tgctgacgaa ggcgcgcgag cacagcgtgg ccctggtggg
     1021 cccggcggcc gaagagctgt tcgacccggt gccggagcag gacctgttcg aggcgctgaa
     1081 cgaaacgctg accctgtgga acagcccgcc ggactgggcg ggtgacgagc gcaacgtggt
     1141 cctgaccctg agccgcatct ggtactccgc ggtcacgggc aagatcgccc cgaaggacgt
     1201 ggcggccgac tgggcgatgg agcgcctgcc ggcgcagtac cagccggtca tcctggaagc
     1261 ccgccaggcc tacctgggcc aggaagagga ccgcctggcc agccgcgccg accagttgga
     1321 agagttcgtc cactacgtca agggcgagat caccaaggtc gtcggtaagt gataacccgg
     1381 gcgccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
     1441 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
     1501 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
     1561 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
     1621 aaacccggga tttatcgtag tgccccaact ggggtaacct gggagttctc tcagttgggg
     1681 gcgtagtcta gacgaagcag ctccagccta cacagctgat taattaactg gcctcatggg
     1741 ccttccgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaacatg
     1801 gtcatagctg tttccttgcg tattgggcgc tctccgcttc ctcgctcact gactcgctgc
     1861 gctcggtcgt tcgggtaaag cctggggtgc ctaatgagca aaaggccagc aaaaggccag
     1921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2821 atctggcccc agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc
     2881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3661 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
