LOCUS       40924_5009        3419 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector patt-saac, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
  PUBMED    24473925
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3419)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3419)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10"; 
            pages: "4557-4570"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label=B-CC
                     /note="B-CC"
     RBS             551..559
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             565..1338
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     misc_recomb     1358..1407
                     /label=P-GG
                     /note="P-GG"
     primer_bind     complement(1414..1433)
     rep_origin      complement(1672..2260)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2434..3291)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3292..3396)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccgg agtacgcgcc
      421 cggggagccc gggggcacgc cctggcaccc gcaccgcggg ataaatggcc gttgacagcc
      481 caagcaaata ctcatataat catagaaaag cggctatatc gctacagctt ccagtaggat
      541 acaagaagtt taaggaggta acaaatgcag tacgagtggc gcaaggcgga gctgatcggc
      601 cagctcctga acctgggtgt cacgccgggt ggcgtgctgc tggtgcacag cagcttccgc
      661 agcgtccgcc cgctggagga cggtccgctg ggcctgatcg aggccctgcg cgcggcgctg
      721 ggtccgggtg gcacgctggt gatgccgagc tggtccggcc tggacgacga accgttcgac
      781 ccggccacca gcccggtcac cccggacctg ggcgtggtga gcgacacgtt ctggcgcctg
      841 ccgaacgtca agcgcagcgc gcacccgttc gcgttcgccg ccgccggtcc gcaggcggag
      901 cagatcatct ccgacccgct gccgctgccg ccgcacagcc cggcgtcccc ggtcgcgcgc
      961 gtccacgagc tggacggtca ggtgctgctg ctgggcgtcg gccacgacgc caacaccacc
     1021 ctgcacctgg ccgagctgat ggccaaggtc ccgtacggcg tcccgcgcca ttgcaccatc
     1081 cttcaggacg gcaagctggt gcgcgtggac tacctggaga acgaccactg ctgcgagcgc
     1141 ttcgcgctgg cggaccgctg gctgaaggaa aagagcctcc agaaggaagg cccggtgggc
     1201 cacgccttcg cccgcctgat ccgctcccgc gacatcgtcg cgaccgccct gggtcaactg
     1261 ggtcgcgacc cgctgatctt cctgcatccg ccggaagccg gctgcgaaga gtgcgacgcg
     1321 gcgcgccagt ccatcggctg ataagatatc atttatcgta gtgccccaac tggggtaacc
     1381 tgggagttct ctcagttggg ggcgtagtct agacgaagca gctccagcct acacagctga
     1441 ttaattaact ggcctcatgg gccttccgct cactgcccgc tttccagtcg ggaaacctgt
     1501 cgtgccagct gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt
     1561 cctcgctcac tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc
     1621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2101 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2221 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2341 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2401 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2461 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2521 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg
     2581 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2641 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2701 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2761 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2821 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2881 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2941 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3001 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3061 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3121 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3181 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3241 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3301 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3361 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
