LOCUS       40924_5004        3667 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector patt-saac-oriT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Myronovskyi M, Rosenkranzer B, Luzhetskyy A.
  TITLE     Iterative marker excision system
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (10), 4557-4570 (2014)
  PUBMED    24473925
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Myronovskyi M, Rosenkraenzer B, Luzhetskyy A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical
            Research Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany
REFERENCE   3  (bases 1 to 3667)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3667)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2014"; volume: "98"; issue: "10"; 
            pages: "4557-4570"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-JUL-2013) AMEG, Helmholtz Institute for Pharmaceutical Research 
            Saarland, Campus, Geb. C2.3 Universitaet des Saarlandes, 
            Saarbruecken, Saarland 66123, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     383..402
     misc_recomb     complement(409..459)
                     /label=B-CC
                     /note="B-CC"
     RBS             551..559
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             565..1338
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     misc_feature    1351..1592
                     /label=oriT
                     /note="oriT"
     misc_recomb     1606..1655
                     /label=P-GG
                     /note="P-GG"
     primer_bind     complement(1662..1681)
     rep_origin      complement(1920..2508)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2682..3539)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3540..3644)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggcctaggc gcgccacagc tgttccgggg atccgtcgac ccggtaccgg agtacgcgcc
      421 cggggagccc gggggcacgc cctggcaccc gcaccgcggg ataaatggcc gttgacagcc
      481 caagcaaata ctcatataat catagaaaag cggctatatc gctacagctt ccagtaggat
      541 acaagaagtt taaggaggta acaaatgcag tacgagtggc gcaaggcgga gctgatcggc
      601 cagctcctga acctgggtgt cacgccgggt ggcgtgctgc tggtgcacag cagcttccgc
      661 agcgtccgcc cgctggagga cggtccgctg ggcctgatcg aggccctgcg cgcggcgctg
      721 ggtccgggtg gcacgctggt gatgccgagc tggtccggcc tggacgacga accgttcgac
      781 ccggccacca gcccggtcac cccggacctg ggcgtggtga gcgacacgtt ctggcgcctg
      841 ccgaacgtca agcgcagcgc gcacccgttc gcgttcgccg ccgccggtcc gcaggcggag
      901 cagatcatct ccgacccgct gccgctgccg ccgcacagcc cggcgtcccc ggtcgcgcgc
      961 gtccacgagc tggacggtca ggtgctgctg ctgggcgtcg gccacgacgc caacaccacc
     1021 ctgcacctgg ccgagctgat ggccaaggtc ccgtacggcg tcccgcgcca ttgcaccatc
     1081 cttcaggacg gcaagctggt gcgcgtggac tacctggaga acgaccactg ctgcgagcgc
     1141 ttcgcgctgg cggaccgctg gctgaaggaa aagagcctcc agaaggaagg cccggtgggc
     1201 cacgccttcg cccgcctgat ccgctcccgc gacatcgtcg cgaccgccct gggtcaactg
     1261 ggtcgcgacc cgctgatctt cctgcatccg ccggaagccg gctgcgaaga gtgcgacgcg
     1321 gcgcgccagt ccatcggctg ataagatatc cgccagcctc gcagagcagg attcccgttg
     1381 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     1441 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     1501 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     1561 atgaccccga agcagggtta tgcagcggaa aagatatcat ttatcgtagt gccccaactg
     1621 gggtaacctg ggagttctct cagttggggg cgtagtctag acgaagcagc tccagcctac
     1681 acagctgatt aattaactgg cctcatgggc cttccgctca ctgcccgctt tccagtcggg
     1741 aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct
     1801 ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc
     1861 taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1921 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1981 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2041 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2101 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2161 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2221 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2281 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2341 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2401 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2461 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2521 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2581 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2641 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2701 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2761 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2821 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2881 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2941 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3001 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3061 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3661 gtgccac
//
