LOCUS       40924_4454       10072 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pamGFP+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Bowring FJ, Yeadon PJ, Catcheside DE.
  TITLE     Use of fluorescent protein to analyse recombination at three loci in
            Neurospora crassa
  JOURNAL   Fungal Genet. Biol. 49 (8), 619-625 (2012)
  PUBMED    22691725
REFERENCE   2  (bases 1 to 10072)
  AUTHORS   Bowring FJ, Yeadon J, Catcheside DEA.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2012) School of Biological Sciences, Flinders 
            University, Sturt Road, Bedford Park, SA 5162, Australia
REFERENCE   3  (bases 1 to 10072)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10072)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2012"; volume: "49"; issue: "8"; pages: 
            "619-625"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUN-2012) School of Biological Sciences, Flinders University, 
            Sturt Road, Bedford Park, SA 5162, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10072
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    336..2090
                     /function="targeting to the am locus"
                     /label=5' truncated am gene
                     /note="5' truncated am gene"
     regulatory      2099..3020
                     /gene="ccg-1"
                     /label=Neurospora crassa ccg-1 (grg-1) promoter
                     /note="Neurospora crassa ccg-1 (grg-1) promoter"
                     /regulatory_class="promoter"
     gene            2099..3020
                     /gene="ccg-1"
                     /label=ccg-1
     primer_bind     3021..3037
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4225..4941
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     complement(4965..4981)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4990..6436
                     /function="targeting to the am locus"
                     /label=3' flank of Neurospora crassa am gene
                     /note="3' flank of Neurospora crassa am gene"
     promoter        complement(6506..6524)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6531..6547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6688..7116
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             7460..8251
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             8272..9129
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9303..9891
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc ggcttgcagg cctacaaggg tacgtctgag
      361 agaaaaacca actgtttagc cgcggacata gagctgactt gatttttcac cacagagttg
      421 gcctacactc tcgagaactc ctccctcttc cagaagcacc ccgagtaccg caccgccctc
      481 accgttgcct ccatccccga gcgtgtcatt cagttccgtg ttgtctggga ggacgacaac
      541 ggcaacgtcc aggtcaaccg cggttaccgt gtccagttca actccgccct cggtccctac
      601 aagggtggtc tccgtctcca cccctccgtc aacctttcca ttctcaagtt cctcggtttc
      661 gagcagatct tcaagaatgc ccttactggc cgtaagtgac cgaacggata tacaaaacca
      721 gaatatcaga tttgctgact cggcgtctct agtgagcatg ggtggtggca agggtggtgc
      781 cgacttcgac cccaagggca agagcgacgc tgagatccgt cgcttctgct gcgctttcat
      841 ggccgagctt cacaagcaca ttggtgctga taccgatgtt cccgctggtg atatcggtgt
      901 tggtggccgt gagatcggtt acatgttcgg tgcctaccgc aaggccgcga accgtttcga
      961 gggtgtcctt actggcaagg gcctctcctg gggtggttcg ctcattcgcc ctgaggccac
     1021 tggttacggt cttgtttact acgtcggcca catgctcgag tactctggcg ccggctccta
     1081 cgctggcaag cgcgttgccc tctccggttc cggcaacgtc gcccagtacg ccgccctcaa
     1141 gctcattgag ctaggcgcca ccgttgtctc cctctccgac tccaagggtg cccttgtcgc
     1201 cactggcgag tccggcatca ccgttgagga catcaacgcc gtcatggcca tcaaggaggc
     1261 ccgtcagtcc cttaccagct tccagcacgc tggccacctc aagtggatcg agggtgcccg
     1321 cccctggctt cacgtcggca aggttgacat cgctcttcct tgcgctaccc agaacgaggt
     1381 ctccaaggag gaggctgagg gtctccttgc cgccggctgc aagttcgtcg ctgagggttc
     1441 caacatgggc tgcactctcg aggccatcga ggtctttgag aacaaccgca aggagaagaa
     1501 gggcgaggcc gtctggtacg cccccggcaa ggccgccaac tgtggtggtg ttgccgtttc
     1561 cggtctcgag atggctcaga acagccagcg cctcaactgg actcaggctg aggttgacga
     1621 gaagctcaag gacatcatga agaacgcctt cttcaacggt ctcaacactg ccaagaccta
     1681 cgtcgaggct gctgagggcg agcttccttc ccttgttgcc ggctccaaca ttgctggttt
     1741 cgtcaaggtt gcccaggcca tgcacgacca gggtgactgg tggtccaaga actaaatggt
     1801 gtcatttcac cgtttaatga gaatggaaag ggaaaaaaag gaaaacttta tgatgggaac
     1861 tgagcaatga aaatcacggg ccggagttgg ggtttgccat gctatcttac cgagcaggct
     1921 tgggttcttt tggcgtttga gtctatttta gacggaggcg ggtttgatga ctttccatgt
     1981 acatatacca ttggtgaatt catatcctac ctgtcgattc acacaagact tgactttctt
     2041 gcaacgcatt cctagtgacc gtgatataaa cttggctgcg tgctcccaat ttcgaagcta
     2101 gaaggagcag tccatctgcg tgaatcacga gagaatcagc tactttgaat cgatggatgc
     2161 agctaccaga agtcactcag ttcgttcaaa gccacatcac tgggcacttc cattgggaca
     2221 ggcattgatc ggacgagacc gacttctggc cgctttcaac agccacatta tatccatgtc
     2281 acggctacgc gtcggccttc ggtaacagaa aagcacacag acagcgattg tgacatggat
     2341 tcgggcaaag cattggtggt cgcaccaggt catcctgagt gtgcagtggc tgctattcag
     2401 atttcatcta actgcgggag aggggttcaa aggggcgtga cgtcacagac aacgggtgaa
     2461 ggacgaagat tgcctcactt ctttgctagc aatttgcctg caaagaaggc gcacatgaca
     2521 agcaaacaac tgggaaacca ctattgaata cccacaatgc aaagctcgga aggtacgtct
     2581 tgattgcagt gtgtcgagtg tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca
     2641 cctcctccac ttctccgagt gccccacccg aacctccagg cgagatggcc ggaacatacc
     2701 atccgcgttg ggattatgac gtatctcctt cttcttcaca tgattccatc ccgttgttgc
     2761 ttgtttgcga gctgtgacgg gagatcgtag atgccacttc gggccaggca ggcagtgcag
     2821 gcagccagga acacaagctt ccaacttggt catctcgatt gccgattaag ggaaccaaat
     2881 gcctatataa gactgtcctc ccacctcccc aataccattc ttttcttctt ccatcatcag
     2941 ccaacaaagc aatcacatct tcactacttc aaatcaacac aacactcaaa ccactttcac
     3001 aacccctcac atcaaccaaa tctagaacta gtggatccac catgcctccc aagaagaccg
     3061 agaccaaggc cgccgatgcc tctgccgccg ccgctcctgc tcccgccgct gctcccacct
     3121 ctgctcccaa gaccaagagc cccagcaccc atgcctctta ccttgtaagc tttacttaat
     3181 ccccaagaga aacgcatctt ttgctaacat tcaaacagga catgatcacg gacgctattg
     3241 ttgcggtatt taaaccccct cattatcttt gttgtggcgt gttttggttg tgggcgcttc
     3301 tctggtcggt gagcaaaatg agacaattgc tctcatgatg cacgcaacca agagtcaccc
     3361 acaccactca ccaccctttt actgtccctc agtcgtgctg attccttctt tttgtcatgc
     3421 tacagctcaa ggaccgcgct ggatctaggt atgtgccatt tttctgctta cttttctcat
     3481 tttttcacca gggctttgcc aagccacacc gcaaaccttg cgggcccttc ctacggtcgc
     3541 accgatgacc actttcattc ttatttccat gacgcgatga aggatcacaa catgttgcta
     3601 acgcacacca cagccgtcaa gctctcaaga agtatgtccg tgccaacaac acgctcggca
     3661 acgtcaccga caacatgttc gactcgctct tcaacaaggc cttgaagaac ggtgtcgaca
     3721 agggtgtctt tgagcagccc aagggtatgt caccacccac atgacgcatc atctccttgt
     3781 attactcttt gcatgctcac acacgatata ggtccttccg gtggcaccaa gcttgccaag
     3841 aaggtcgcta agcctgctcc caagaaggct gctcccaaga aggagaccaa ggagaagaag
     3901 cccgctgccg ctaagaagga gggtgccgcc aagaaggaga ccaaggagaa gaaggctcct
     3961 gctgccaaga aggcggctgc ccccaagaag gctgctgctc ccaagaagga ggtcaaggag
     4021 aagaaggcgg ctgcccccaa gaagaaggcc gctgctcctg ccgttgccga caaggagacc
     4081 gtcctcacca agaccaagtc tggccgtgtc gccaagagca ccgctaagcc tgctgccgcc
     4141 aagaaggccg ctgctcccaa gaaggctgcc gccagcaaga aggccgagaa ggccgagccc
     4201 gctgccgaga aggcattaat taacatggtg agcaagggcg aggagctgtt caccggggtg
     4261 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4321 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4381 aagctgcccg tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc
     4441 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4501 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4561 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4621 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4681 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4741 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4801 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     4861 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4921 ggcatggacg agctgtacaa gtaagaattc gatatcaagc ttatcgatac cgtcgacctc
     4981 gggcgcgccg ctgttacaaa atcccgaacc tcttccaggc catgtcaaat gttggtaccc
     5041 gcagctttgg cagaaatcag ggtaatactg cgtataaatc atcgatgacc tcttgcgccg
     5101 cagacaacac ctgatcatgt cattcttgtt gaagattgaa accaattttg gagtttcaga
     5161 gattccgatg tcgcccgttt ctgtgattac tgacaacctc tcttcacttt gactcatcac
     5221 tatcaacaca tcgtcaagta cttttttgta caaaaatggg ccgcaaatct gatccggatg
     5281 atattctgaa cgtaagtagc ggcagtcagg cgtcgttggc atgactcgag atgtgcgctg
     5341 tctgatgtcg agggtcgagg atgttgctgg atcacactcg cgggcaccgt acatacatgg
     5401 atcccatgtg ctttgagacg atgctctctt tgtttttctt gttttttttc tttcactctt
     5461 tttgtcgagt ctttctgttc ctctgttttt ctccgattcg atacggcggc tttcggagat
     5521 gggggccgac tcatcacagc ttttccagcg tatggatgta caagaggctc gcattccgtc
     5581 actgtatcgt agctggtgag ctggagtatt ggagtatctg atcccattgg actattgata
     5641 gtgagagagg tagggtctta attggacact aacagtttcc tgtacttagc gcggtcttaa
     5701 gagttggtgc aagagtaaac aactagcgac aaacaaggag gttgcctaat ccccgtgccc
     5761 ttgttcagct tcctctgtac aacttgagac cgaaaggggt gatgatgatc caaaaaaaaa
     5821 cactgtgaag gagttgggga agcaccgaac ttaaaagtcc agggggttga aacaatgttg
     5881 tggtggcgat gccttcctcc ttcaacatac tcttcatcgt agcaatatcg atatcttggc
     5941 acaaatgatc tcgccacgca tttccagatt cgccaatcct cggacggcac aaccacacct
     6001 ccccacgggg aacagcttgg tgtcaccttg tgttggtgct actctttcca ttcctgtctc
     6061 atcaggtgcc tgataaccca agaaccaaaa cctttgaggc ttcattcact ttgaacccag
     6121 taaacaggga attacgaaac gaaacgagaa cgggtgtaac cagaggtcaa ccttgccatg
     6181 acttgttttg tccgaattgg gaccagatag gggaagaaaa aaagaaaaag agaaaacaaa
     6241 taattggata agtagagagg gacaagctgg ctcgggacga tacaaggaat catgcaattg
     6301 ggacttgaat gagaacacag gcatcacgag ggagcctgct tgctggggaa cacgaaagcg
     6361 atattcttgg atgtcagcac ttttggactg acatttcaca gagcaaggta cggtgtgatg
     6421 cgatgttggt gtcactaagc cgaattctgc agatatccat cacactggcg gccgctcgag
     6481 catgcatcta gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     6541 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     6601 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6661 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     6721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6781 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6841 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6901 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6961 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7021 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7081 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaattcag ggcgcaaggg
     7141 ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
     7201 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
     7261 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
     7321 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
     7381 gatggctttc ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac
     7441 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7501 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7561 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7621 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7681 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7741 gggcgaagtg ccggggcagg atctcctgtc atcccacctt gctcctgccg agaaagtatc
     7801 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7861 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7921 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7981 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     8041 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     8101 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     8161 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     8221 cgccttctat cgccttcttg acgagttctt ctgaattgaa aaaggaagag tatgagtatt
     8281 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8341 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8401 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8461 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8521 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8581 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8641 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8701 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8761 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8821 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8881 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8941 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9001 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9061 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9121 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9961 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10021 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
