LOCUS       V012788                 6215 bp    DNA     circular SYN 17-NOV-2021
DEFINITION  Exported.
ACCESSION   V012788
VERSION     V012788
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        385..688
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        689..892
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     928..944
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(978..994)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     polyA_signal    complement(1014..1148)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1716..2135
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2201..3535)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(4388..4976)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5150..6007)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6008..6112)
                     /label="AmpR promoter"
ORIGIN
        1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
       61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
      121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
      181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
      241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
      301 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatattt
      361 gtctagggag atcagcctgc aggtcgttac ataacttacg gtaaatggcc cgcctggctg
      421 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      481 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      541 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      601 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      661 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      721 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      781 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      841 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      901 gaaccgtcag ttggtaccgt ttaaactcga ggtcgacggt atcgataagc ttgatatcga
      961 attcctgcag cccgggggat ccactagttc tagagcggcc gccacagcgg ggagatccag
     1021 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1081 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1141 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1201 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg
     1261 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1321 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1381 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gtccccgcgg tggcagatct
     1441 ggaaggtgct gaggtacgat gagacccgca ccaggtgcag accctgcgag tgtggcggta
     1501 aacatattag gaaccagcct gtgatgctgg atgtgaccga ggagctgagg cccgatcact
     1561 tggtgctggc ctgcacccgc gctgagtttg gctctagcga tgaagataca gattgaggta
     1621 ctgaaatgtg tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt
     1681 agttttgtat ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa
     1741 gcattgtgag ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg
     1801 tgatgggctc cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct
     1861 acgagaccgt gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg
     1921 cagccaccgc ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg
     1981 cagcttcccg ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt
     2041 ctttgacccg ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt
     2101 ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact
     2161 ctgtttggat ttggatcaag caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc
     2221 ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct gtgtattttt tccaggacgt
     2281 ggtaaaggtg actctggatg ttcagataca tgggcataag cccgtctctg gggtggaggt
     2341 agcaccactg cagagcttca tgctgcgggg tggtgttgta gatgatccag tcgtagcagg
     2401 agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc
     2461 ccttggtgta agtgtttaca aagcggttaa gctgggatgg gtgcatacgt ggggatatga
     2521 gatgcatctt ggactgtatt tttaggttgg ctatgttccc agccatatcc ctccggggat
     2581 tcatgttgtg cagaaccacc agcacagtgt atccggtgca cttgggaaat ttgtcatgta
     2641 gcttagaagg aaatgcgtgg aagaacttgg agacgccctt gtgacctcca agattttcca
     2701 tgcattcgtc cataatgatg gcaatgggcc cacgggcggc ggcctgggcg aagatatttc
     2761 tgggatcact aacgtcatag ttgtgttcca ggatgagatc gtcataggcc atttttacaa
     2821 agcgcgggcg gagggtgcca gactgcggta taatggttcc atccggccca ggggcgtagt
     2881 taccctcaca gatttgcatt tcccacgctt tgagttcaga tggggggatc atgtctacct
     2941 gcggggcgat gaagaaaacg gtttccgggg taggggagat cagctgggaa gaaagcaggt
     3001 tcctgagcag ctgcgactta ccgcagccgg tgggcccgta aatcacacct attaccgggt
     3061 gcaactggta gttaagagag ctgcagctgc cgtcatccct gagcaggggg gccacttcgt
     3121 taagcatgtc cctgactcgc atgttttccc tgaccaaatc cgccagaagg cgctcgccgc
     3181 ccagcgatag cagttcttgc aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg
     3241 taggcatgct tttgagcgtt tgaccaagca gttccaggcg gtcccacagc tcggtcacct
     3301 gctctacggc atctcgatcc agcatatctc ctcgtttcgc gggttggggc ggctttcgct
     3361 gtacggcagt agtcggtgct cgtccagacg ggccagggtc atgtctttcc acgggcgcag
     3421 ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc gctccgggct gcgcgctggc
     3481 cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc
     3541 gtcggccagg tagcatttga ccatggtgtc atagtccagc ccctccgcgg cgtggccctt
     3601 ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg cagtgcagac ttttgagggc
     3661 gtagagcttg ggcgcgagaa ataccgattc cggggagtag gcatccgcgc cgcaggcccc
     3721 gcagacggtc tcgcattcca cgagccaggt gagctctggc cgttcggggt caaaaaccag
     3781 gtttccccca tgctttttga tgcgtttctt acctctggtt tccatgagcc ggtgtccacg
     3841 ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgac
     3901 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     3961 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     4021 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     4081 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     4141 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     4201 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     4261 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     4321 accatcaggg acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4381 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4441 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4501 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4561 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4621 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4681 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4741 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4801 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     4861 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4921 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4981 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5041 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5101 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5161 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5221 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5281 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5341 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5401 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5461 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5521 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5581 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5641 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5701 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5761 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5821 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5881 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5941 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6001 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6061 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6121 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6181 ataggcgtat cacgaggccc tttcgtcttc aagaa
//
