LOCUS       V012787                 6935 bp    DNA     circular SYN 17-NOV-2021
DEFINITION  Exported.
ACCESSION   V012787
VERSION     V012787
KEYWORDS    pacAd5 CMV-GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        385..688
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        689..892
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    926..978
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             992..1708
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    complement(1732..1866)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             2436..2855
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2921..4255)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(5108..5696)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5870..6727)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6728..6832)
                     /label="AmpR promoter"
ORIGIN
        1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
       61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
      121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
      181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
      241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
      301 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatattt
      361 gtctagggag atcagcctgc aggtcgttac ataacttacg gtaaatggcc cgcctggctg
      421 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      481 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      541 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      601 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      661 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      721 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      781 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      841 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      901 gaaccgtcag atggtaccgt ttaaactcga gctcaagctt cgaattctgc agtcgacggt
      961 accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
     1021 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1081 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1141 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     1201 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1261 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1321 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1381 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1441 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1501 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1561 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1621 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1681 cactctcggc atggacgagc tgtacaagta aagcggccgc cacagcgggg agatccagac
     1741 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     1801 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     1861 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     1921 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcccggctgc
     1981 ctcgcgcgtt tcggtgatga cggtgaaaac ctcttgacac atgcagctcc cggagacggt
     2041 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     2101 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gtccccgcgg tggcagatct
     2161 ggaaggtgct gaggtacgat gagacccgca ccaggtgcag accctgcgag tgtggcggta
     2221 aacatattag gaaccagcct gtgatgctgg atgtgaccga ggagctgagg cccgatcact
     2281 tggtgctggc ctgcacccgc gctgagtttg gctctagcga tgaagataca gattgaggta
     2341 ctgaaatgtg tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt
     2401 agttttgtat ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa
     2461 gcattgtgag ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg
     2521 tgatgggctc cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct
     2581 acgagaccgt gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg
     2641 cagccaccgc ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg
     2701 cagcttcccg ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt
     2761 ctttgacccg ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt
     2821 ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact
     2881 ctgtttggat ttggatcaag caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc
     2941 ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct gtgtattttt tccaggacgt
     3001 ggtaaaggtg actctggatg ttcagataca tgggcataag cccgtctctg gggtggaggt
     3061 agcaccactg cagagcttca tgctgcgggg tggtgttgta gatgatccag tcgtagcagg
     3121 agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc
     3181 ccttggtgta agtgtttaca aagcggttaa gctgggatgg gtgcatacgt ggggatatga
     3241 gatgcatctt ggactgtatt tttaggttgg ctatgttccc agccatatcc ctccggggat
     3301 tcatgttgtg cagaaccacc agcacagtgt atccggtgca cttgggaaat ttgtcatgta
     3361 gcttagaagg aaatgcgtgg aagaacttgg agacgccctt gtgacctcca agattttcca
     3421 tgcattcgtc cataatgatg gcaatgggcc cacgggcggc ggcctgggcg aagatatttc
     3481 tgggatcact aacgtcatag ttgtgttcca ggatgagatc gtcataggcc atttttacaa
     3541 agcgcgggcg gagggtgcca gactgcggta taatggttcc atccggccca ggggcgtagt
     3601 taccctcaca gatttgcatt tcccacgctt tgagttcaga tggggggatc atgtctacct
     3661 gcggggcgat gaagaaaacg gtttccgggg taggggagat cagctgggaa gaaagcaggt
     3721 tcctgagcag ctgcgactta ccgcagccgg tgggcccgta aatcacacct attaccgggt
     3781 gcaactggta gttaagagag ctgcagctgc cgtcatccct gagcaggggg gccacttcgt
     3841 taagcatgtc cctgactcgc atgttttccc tgaccaaatc cgccagaagg cgctcgccgc
     3901 ccagcgatag cagttcttgc aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg
     3961 taggcatgct tttgagcgtt tgaccaagca gttccaggcg gtcccacagc tcggtcacct
     4021 gctctacggc atctcgatcc agcatatctc ctcgtttcgc gggttggggc ggctttcgct
     4081 gtacggcagt agtcggtgct cgtccagacg ggccagggtc atgtctttcc acgggcgcag
     4141 ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc gctccgggct gcgcgctggc
     4201 cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc
     4261 gtcggccagg tagcatttga ccatggtgtc atagtccagc ccctccgcgg cgtggccctt
     4321 ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg cagtgcagac ttttgagggc
     4381 gtagagcttg ggcgcgagaa ataccgattc cggggagtag gcatccgcgc cgcaggcccc
     4441 gcagacggtc tcgcattcca cgagccaggt gagctctggc cgttcggggt caaaaaccag
     4501 gtttccccca tgctttttga tgcgtttctt acctctggtt tccatgagcc ggtgtccacg
     4561 ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgac
     4621 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     4681 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     4741 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     4801 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     4861 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     4921 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     4981 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     5041 accatcaggg acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5521 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5641 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5701 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5761 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5821 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5881 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5941 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6001 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6061 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6121 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6181 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6241 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6301 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6361 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6421 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6481 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6541 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6601 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6661 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6721 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6781 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6841 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6901 ataggcgtat cacgaggccc tttcgtcttc aagaa
//
