LOCUS       V013335                 7480 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013335
VERSION     V013335
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(60..435)
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(620..724)
                     /label="AmpR promoter"
     repeat_region   838..940
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     1028..1178
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     1212..1228
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1447..1658
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             2176..2892
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    complement(3518..3652)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             4220..4639
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(4705..6039)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(6892..7480)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tccatccagt ctataattgt gccggaagct agagtagtag tccccagtta tagttgcgca
       61 acgttgtgcc atgctgcagg catcgtgtgt cacgctcgtc gtttggtatg gcttcattca
      121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      361 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
      421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      721 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
      781 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaattaatt aagcaagcat
      841 catcaataat ataccttatt ttggattgaa gccaatatga taatgagggg gtggagtttg
      901 tgacgtggcg cggggcgtgg gaacggggcg ggtgacgtag tagtgtggcg gaagtgtgat
      961 gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac gtttttggtg
     1021 tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat gttgtagtaa
     1081 atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat aagaggaagt
     1141 gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg agatccggta
     1201 ccgtttaaac tcgaggtcga cggtatcgat gcctccccgt caccaccccc cccaacccgc
     1261 cccgaccgga gctgagagta attcatacaa aaggactcgc ccctgccttg gggaatccca
     1321 gggaccgtcg ttaaactccc actaacgtag aacccagaga tcgctgcgtt cccgccccct
     1381 cacccgcccg ctctcgtcat cactgaggtg gagaagagca tgcgtgaggc tccggtgccc
     1441 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     1501 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     1561 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     1621 acgttctttt tcgcaacggg tttgccgcca gaacacaggt acacatattg accaaatcag
     1681 ggtaattttg catttgtaat tttaaaaaat gctttcttct tttaatatac ttttttgttt
     1741 atcttatttc taatactttc cctaatctct ttctttcagg gcaataatga tacaatgtat
     1801 catgcctctt tgcaccattc taaagaataa cagtgataat ttctgggtta aggcaatagc
     1861 cgattagttc tcgaggatcc gactgaagtc gctagctcga gcttttggag aatatttctg
     1921 catataaata tttctgcata taaattgtaa ctgatgtaag aggtttcata ttgctaatag
     1981 cagctacaat ccagctacca ttctgctttt attttatggt tgggataagg ctggattatt
     2041 ctgagtccaa gctaggccct tttgctaatc atgttcatac ctcttatctt cctcccacag
     2101 ctcctgggca acgtgctggt ctgtgtgctg gcccatcact ttggcaaagc acgtgagatt
     2161 gaattctgac acaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2221 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2281 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2341 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2401 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2461 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2521 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2581 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2641 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     2701 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2761 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     2821 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2881 gagctgtaca aggccaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     2941 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3001 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     3061 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     3121 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     3181 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     3241 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     3301 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     3361 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     3421 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     3481 cgcaagcccg gtgcctgagc ggccgccacc gcggggagat ccagacatga taagatacat
     3541 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3601 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3661 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     3721 gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt
     3781 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     3841 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     3901 ggcgcagcca tgaggtcgac tctagtcccc gcggtggcag atctggaagg tgctgaggta
     3961 cgatgagacc cgcaccaggt gcagaccctg cgagtgtggc ggtaaacata ttaggaacca
     4021 gcctgtgatg ctggatgtga ccgaggagct gaggcccgat cacttggtgc tggcctgcac
     4081 ccgcgctgag tttggctcta gcgatgaaga tacagattga ggtactgaaa tgtgtgggcg
     4141 tggcttaagg gtgggaaaga atatataagg tgggggtctt atgtagtttt gtatctgttt
     4201 tgcagcagcc gccgccgcca tgagcaccaa ctcgtttgat ggaagcattg tgagctcata
     4261 tttgacaacg cgcatgcccc catgggccgg ggtgcgtcag aatgtgatgg gctccagcat
     4321 tgatggtcgc cccgtcctgc ccgcaaactc tactaccttg acctacgaga ccgtgtctgg
     4381 aacgccgttg gagactgcag cctccgccgc cgcttcagcc gctgcagcca ccgcccgcgg
     4441 gattgtgact gactttgctt tcctgagccc gcttgcaagc agtgcagctt cccgttcatc
     4501 cgcccgcgat gacaagttga cggctctttt ggcacaattg gattctttga cccgggaact
     4561 taatgtcgtt tctcagcagc tgttggatct gcgccagcag gtttctgccc tgaaggcttc
     4621 ctcccctccc aatgcggttt aaaacataaa taaaaaacca gactctgttt ggatttggat
     4681 caagcaagtg tcttgctgtc tttatttagg ggttttgcgc gcgcggtagg cccgggacca
     4741 gcggtctcgg tcgttgaggg tcctgtgtat tttttccagg acgtggtaaa ggtgactctg
     4801 gatgttcaga tacatgggca taagcccgtc tctggggtgg aggtagcacc actgcagagc
     4861 ttcatgctgc ggggtggtgt tgtagatgat ccagtcgtag caggagcgct gggcgtggtg
     4921 cctaaaaatg tctttcagta gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt
     4981 tacaaagcgg ttaagctggg atgggtgcat acgtggggat atgagatgca tcttggactg
     5041 tatttttagg ttggctatgt tcccagccat atccctccgg ggattcatgt tgtgcagaac
     5101 caccagcaca gtgtatccgg tgcacttggg aaatttgtca tgtagcttag aaggaaatgc
     5161 gtggaagaac ttggagacgc ccttgtgacc tccaagattt tccatgcatt cgtccataat
     5221 gatggcaatg ggcccacggg cggcggcctg ggcgaagata tttctgggat cactaacgtc
     5281 atagttgtgt tccaggatga gatcgtcata ggccattttt acaaagcgcg ggcggagggt
     5341 gccagactgc ggtataatgg ttccatccgg cccaggggcg tagttaccct cacagatttg
     5401 catttcccac gctttgagtt cagatggggg gatcatgtct acctgcgggg cgatgaagaa
     5461 aacggtttcc ggggtagggg agatcagctg ggaagaaagc aggttcctga gcagctgcga
     5521 cttaccgcag ccggtgggcc cgtaaatcac acctattacc ggctgcaact ggtagttaag
     5581 agagctgcag ctgccgtcat ccctgagcag gggggccact tcgttaagca tgtccctgac
     5641 tcgcatgttt tccctgacca aatccgccag aaggcgctcg ccgcccagcg atagcagttc
     5701 ttgcaaggaa gcaaagtttt tcaacggttt gagaccgtcc gccgtaggca tgcttttgag
     5761 cgtttgacca agcagttcca ggcggtccca cagctcggtc acctgctcta cggcatctcg
     5821 atccagcata tctcctcgtt tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg
     5881 tgctcgtcca gacgggccag ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta
     5941 gtctgggtca cggtgaaggg gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg
     6001 ctggtcctgc tggtgctgaa gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat
     6061 ttgaccatgg tgtcatagtc cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc
     6121 ttggaggagg cgccgcacga ggggcagtgc agacttttga gggcgtagag cttgggcgcg
     6181 agaaataccg attccgggga gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat
     6241 tccacgagcc aggtgagctc tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt
     6301 ttgatgcgtt tcttacctct ggtttccatg agccggtgtc cacgctcggt gacgaaaagg
     6361 ctgtccgtgt ccccgtatac agacttgaga ggcctgtcct cgaccgatgc ccttgagagc
     6421 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     6481 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     6541 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     6601 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     6661 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     6721 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     6781 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     6841 tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6901 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6961 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7021 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7081 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7141 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7201 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7261 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7321 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7381 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcgggg ggttttttgt
     7441 ttgcaagcag cagattacgc gcagaaaaaa aggatctcac
//
