LOCUS       40924_4989        5467 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector paT7P-3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium 
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27794600
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of 
            Science and Technology of China, University of Science and 
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 5467)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5467)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2016) School of Life Sciences, University of Science and 
            Technology of China, University of Science and Technology of China, 
            No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China, 
            Hefei, Anhui 230026, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0 
            Assembly Name         :: paT7P-3
            Coverage              :: 5467bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..116)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      125..162
                     /regulatory_class="promoter"
     regulatory      179..190
                     /regulatory_class="ribosome_binding_site"
     RBS             179..190
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1925..2374
                     /label=pMag
                     /note="'positive Magnet' variant of an N-terminally
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
     regulatory      3371..3404
                     /regulatory_class="terminator"
     misc_feature    3404..3424
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3425..3482
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(3677..4265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4501..5313)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
      121 tagcggcacg taagaggttc caactttcac cataatgaaa caccatgggg tactagagaa
      181 agaggagaaa agatctatga acacgattaa catcgctaag aacgacttct ctgacatcga
      241 actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga
      301 acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt
      361 tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac
      421 taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa
      481 gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa atcaagccgg aagccgtagc
      541 gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca
      601 ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg
      661 tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg
      721 gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct
      781 actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg
      841 ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg
      901 cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc
      961 aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc
     1021 taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc
     1081 gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga
     1141 ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct
     1201 agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat
     1261 tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac
     1321 cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg
     1381 tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg
     1441 gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca
     1501 aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga
     1561 aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt
     1621 ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc
     1681 tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg
     1741 ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc
     1801 gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg
     1861 tggtcgcgcg gttaacttgc ttcctagtga attcggcggc agtggagggt ctggtggcag
     1921 cggacatacg ctctacgctc ccggcggtta tgacattatg ggctatctga ggcagattag
     1981 gaacaggcca aacccccaag tagaactggg acctgttgac acgtcatgcg ctctgattct
     2041 gtgcgacctg aagcaaaaag acacgccaat tgtgtacgcc tcggaagctt ttctctatat
     2101 gacaggatac agcaatgcgg aggtcttggg gagaaactgc cgttttcttc agtcacccga
     2161 cggaatggtc aagccgaaat cgacaaggaa gtacgtcgac tccaacacga tcaatacgat
     2221 gaggaaagcg attgatagga acgccgaggt gcaggttgag gtggtcaatt ttaagaagaa
     2281 cggccaacgg tttgtcaact tcttgacgat gattccggtg cgagatgaaa caggggaata
     2341 ccggtacagc atgggtttcc agtgcgaaac ggaagggggt tctgggggga gcggcggggg
     2401 atccgaaacc gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca
     2461 agcagacgca atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg
     2521 tgaaatctct gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta
     2581 cggtgttact cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga
     2641 gttcggcttc cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa
     2701 gggtctgatg ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga
     2761 atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc
     2821 taagctgctg gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg
     2881 cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat
     2941 tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac
     3001 caacaaagat agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt
     3061 acacagccaa gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg
     3121 aatcgaatct tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa
     3181 cctgttcaaa gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc
     3241 tgatttctac gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc
     3301 acttccggct aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc
     3361 gtaaccgcgg cgccgaaaac cccgcttcgg cggggttttg ccgcactagt agcggccgct
     3421 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
     3481 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3661 ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac
     3721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga
     4441 gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta
     4501 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     4561 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     4621 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     4681 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     4741 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc
     4801 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     4861 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     4921 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     4981 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     5041 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     5101 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     5161 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     5221 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     5281 ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     5341 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
     5401 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca
     5461 gctcgag
//
