LOCUS       40924_4984        5502 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector paT7P-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium 
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27794600
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of 
            Science and Technology of China, University of Science and 
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 5502)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2016) School of Life Sciences, University of Science and 
            Technology of China, University of Science and Technology of China, 
            No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China, 
            Hefei, Anhui 230026, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0 
            Assembly Name         :: paT7P-2
            Coverage              :: 5502bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..116)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      125..168
                     /regulatory_class="promoter"
     regulatory      179..190
                     /regulatory_class="ribosome_binding_site"
     RBS             179..190
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1925..2374
                     /label=pMag
                     /note="'positive Magnet' variant of an N-terminally
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
     regulatory      2419..2430
                     /regulatory_class="ribosome_binding_site"
     RBS             2419..2430
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2437..3399
                     /codon_start=1
                     /product="C565"
                     /label=C565
                     /note="C565 fragment of T7 RNAP"
                     /protein_id="APD28472.1"
                     /translation="METVQDIYGIVAKKVNEILQADAINGTDNEVVTVTDENTGEISEK
                     VKLGTKALAGQWLAYGVTRSVTKRSVMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMF
                     TQPNQAAGYMAKLIWESVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVH
                     WVTPDGFPVWQEYKKPIQTRLNLMFLGQFRLQPTINTNKDSEIDAHKQESGIAPNFVHS
                     QDGSHLRKTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVLAD
                     FYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA"
     regulatory      3406..3439
                     /regulatory_class="terminator"
     misc_feature    3439..3459
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3460..3517
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(3712..4300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4536..5348)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
      121 tagcggcacg taagaggttc caactttcac cataatgaaa caccatgggg tactagagaa
      181 agaggagaaa agatctatga acacgattaa catcgctaag aacgacttct ctgacatcga
      241 actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga
      301 acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt
      361 tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac
      421 taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa
      481 gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa atcaagccgg aagccgtagc
      541 gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca
      601 ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg
      661 tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg
      721 gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct
      781 actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg
      841 ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg
      901 cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc
      961 aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc
     1021 taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc
     1081 gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga
     1141 ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct
     1201 agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat
     1261 tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac
     1321 cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg
     1381 tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg
     1441 gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca
     1501 aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga
     1561 aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt
     1621 ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc
     1681 tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg
     1741 ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc
     1801 gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg
     1861 tggtcgcgcg gttaacttgc ttcctagtga attcggcggc agtggagggt ctggtggcag
     1921 cggacatacg ctctacgctc ccggcggtta tgacattatg ggctatctga ggcagattag
     1981 gaacaggcca aacccccaag tagaactggg acctgttgac acgtcatgcg ctctgattct
     2041 gtgcgacctg aagcaaaaag acacgccaat tgtgtacgcc tcggaagctt ttctctatat
     2101 gacaggatac agcaatgcgg aggtcttggg gagaaactgc cgttttcttc agtcacccga
     2161 cggaatggtc aagccgaaat cgacaaggaa gtacgtcgac tccaacacga tcaatacgat
     2221 gaggaaagcg attgatagga acgccgaggt gcaggttgag gtggtcaatt ttaagaagaa
     2281 cggccaacgg tttgtcaact tcttgacgat gattccggtg cgagatgaaa caggggaata
     2341 ccggtacagc atgggtttcc agtgcgaaac ggaataataa ggtaccacta gagcttgtgc
     2401 aagaagcgta ccaacgttaa agaggagaaa cgacatatgg aaaccgttca ggacatctac
     2461 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat
     2521 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg
     2581 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag
     2641 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg
     2701 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat
     2761 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct
     2821 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa
     2881 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat
     2941 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc
     3001 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca
     3061 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt
     3121 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac
     3181 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact
     3241 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac
     3301 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac
     3361 ctccgtgaca tcttagagtc ggacttcgcg ttcgcgtaac cgcggcgccg aaaaccccgc
     3421 ttcggcgggg ttttgccgca ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt
     3481 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     3541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
     3721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4441 aagtatatat gagtaaactt ggtctgacag ctcgagtccc gtcaagtcag cgtaatgctc
     4501 tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     4561 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     4621 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     4681 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     4741 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     4801 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     4861 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     4921 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     4981 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     5041 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     5101 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     5161 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     5221 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     5281 aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc ccgttgaata
     5341 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     5401 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     5461 tgaataaatc gaacttttgc tgagttgaag gatcagctcg ag
//
