LOCUS       40924_4979        6012 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector paT7P-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium 
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27794600
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of 
            Science and Technology of China, University of Science and 
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 6012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2016) School of Life Sciences, University of Science and 
            Technology of China, University of Science and Technology of China, 
            No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China, 
            Hefei, Anhui 230026, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0 
            Assembly Name         :: paT7P-1
            Coverage              :: 6012bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..116)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      125..162
                     /regulatory_class="promoter"
     regulatory      179..190
                     /regulatory_class="ribosome_binding_site"
     RBS             179..190
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1925..2374
                     /label=pMag
                     /note="'positive Magnet' variant of an N-terminally
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
     regulatory      2419..2430
                     /regulatory_class="ribosome_binding_site"
     RBS             2419..2430
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2470..2919
                     /label=pMag
                     /note="'positive Magnet' variant of an N-terminally
                     truncated Vivid (VVD) blue light photoreceptor from 
                     Neurospora crassa (Kawano et al., 2015)"
     regulatory      3916..3949
                     /regulatory_class="terminator"
     misc_feature    3949..3969
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3970..4027
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(4222..4810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5046..5858)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
       61 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc
      121 tagcggcacg taagaggttc caactttcac cataatgaaa caccatgggg tactagagaa
      181 agaggagaaa agatctatga acacgattaa catcgctaag aacgacttct ctgacatcga
      241 actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt tagctcgcga
      301 acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc gcaagatgtt
      361 tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc ctctcatcac
      421 taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag tgaaagctaa
      481 gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa atcaagccgg aagccgtagc
      541 gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata caaccgttca
      601 ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg gtcgtatccg
      661 tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca agcgcgtagg
      721 gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct ctaagggtct
      781 actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg taggagtacg
      841 ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc aaaatgctgg
      901 cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg aggctatcgc
      961 aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg tagttcctcc
     1021 taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc gtcctctggc
     1081 gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt acatgcctga
     1141 ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca agaaagtcct
     1201 agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca tccctgcgat
     1261 tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg aggctctcac
     1321 cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca agtctcgccg
     1381 tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata aggccatctg
     1441 gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt tcaacccgca
     1501 aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa tcggtaagga
     1561 aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata aggttccgtt
     1621 ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt gcgctaagtc
     1681 tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc ttgcgttctg
     1741 ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc ttccgctggc
     1801 gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag atgaggtagg
     1861 tggtcgcgcg gttaacttgc ttcctagtga attcggcggc agtggagggt ctggtggcag
     1921 cggacatacg ctctacgctc ccggcggtta tgacattatg ggctatctga ttcagattat
     1981 gaacaggcca aacccccaag tagaactggg acctgttgac acgtcatgcg ctctgattct
     2041 gtgcgacctg aagcaaaaag acacgccaat tgtgtacgcc tcggaagctt ttctctatat
     2101 gacaggatac agcaatgcgg aggtcttggg gagaaactgc cgttttcttc agtcacccga
     2161 cggaatggtc aagccgaaat cgacaaggaa gtacgtcgac tccaacacga tcaatacgat
     2221 gaggaaagcg attgatagga acgccgaggt gcaggttgag gtggtcaatt ttaagaagaa
     2281 cggccaacgg tttgtcaact tcttgacgat gattccggtg cgagatgaaa caggggaata
     2341 ccggtacagc atgggtttcc agtgcgaaac ggaataataa ggtaccacta gagcttgtgc
     2401 aagaagcgta ccaacgttaa agaggagaaa cgacatatgg gcggcagtgg agggtctggt
     2461 ggcagcggac atacgctcta cgctcccggc ggttatgaca ttatgggcta tctgattcag
     2521 attatgaaca ggccaaaccc ccaagtagaa ctgggacctg ttgacacgtc atgcgctctg
     2581 attctgtgcg acctgaagca aaaagacacg ccaattgtgt acgcctcgga agcttttctc
     2641 tatatgacag gatacagcaa tgcggaggtc ttggggagaa actgccgttt tcttcagtca
     2701 cccgacggaa tggtcaagcc gaaatcgaca aggaagtacg tcgactccaa cacgatcaat
     2761 acgatgagga aagcgattga taggaacgcc gaggtgcagg ttgaggtggt caattttaag
     2821 aagaacggcc aacggtttgt caacttcttg acgatgattc cggtgcgaga tgaaacaggg
     2881 gaataccggt acagcatggg tttccagtgc gaaacggaag ggggttctgg ggggagcggc
     2941 gggggatccg aaaccgttca ggacatctac gggattgttg ctaagaaagt caacgagatt
     3001 ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac cgatgagaac
     3061 actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg tcaatggctg
     3121 gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc
     3181 aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc
     3241 ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt
     3301 tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct
     3361 gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag
     3421 cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag
     3481 cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt
     3541 aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac
     3601 tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag
     3661 tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct
     3721 gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta
     3781 ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg
     3841 ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc ggacttcgcg
     3901 ttcgcgtaac cgcggcgccg aaaaccccgc ttcggcgggg ttttgccgca ctagtagcgg
     3961 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc
     4021 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt
     4081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4201 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa
     4261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4621 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4981 ctcgagtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt aaccaattct
     5041 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     5101 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     5161 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     5221 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     5281 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
     5341 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     5401 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     5461 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     5521 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     5581 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     5641 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     5701 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     5761 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     5821 ggcctggagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     5881 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     5941 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     6001 gatcagctcg ag
//
