LOCUS       40924_48593        3331 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing ZsYellow1 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..981
                     /codon_start=1
                     /label=ZsYellow1
                     /note="Zoanthus yellow fluorescent protein"
                     /translation="MAHSKHGLKEEMTMKYHMEGCVNGHKFVITGEGIGYPFKGKQTIN
                     LCVIEGGPLPFSEDILSAGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWGRSFLFEDGAV
                     CICNVDITVSVKENCIYHKSIFNGVNFPADGPVMKKMTTNWEASCEKIMPVPKQGILKG
                     DVSMYLLLKDGGRYRCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKWQLTEH
                     AIAFPSALA"
     promoter        1426..1530
                     /label=AmpR promoter
     CDS             1531..2388
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2562..3150
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggctcattca
      301 aagcacggtc taaaagaaga aatgacaatg aaataccaca tggaagggtg cgtcaacgga
      361 cataaatttg tgatcacggg cgaaggcatt ggatatccgt tcaaagggaa acagactatt
      421 aatctgtgtg tgatcgaagg gggaccattg ccattttccg aagacatatt gtcagctggc
      481 tttaagtacg gagacaggat tttcactgaa tatcctcaag acatagtaga ctatttcaag
      541 aactcgtgtc ctgctggata tacatggggc aggtcttttc tctttgagga tggagcagtc
      601 tgcatatgca atgtagatat aacagtgagt gtcaaagaaa actgcattta tcataagagc
      661 atatttaatg gagtgaattt tcctgctgat ggacctgtga tgaaaaagat gacaactaac
      721 tgggaagcat cctgcgagaa gatcatgcca gtacctaagc aggggatact gaaaggggat
      781 gtctccatgt acctccttct gaaggatggt gggcgttacc ggtgccagtt cgacacagtt
      841 tacaaagcaa agtctgtgcc aagtaagatg ccggagtggc acttcatcca gcataagctc
      901 ctccgtgaag accgcagcga tgctaagaat cagaagtggc agctgacaga gcatgctatt
      961 gcattccctt ctgccttggc ctgaagcggc cgcgactcta gaattccaac tgagcgccgg
     1021 tcgctaccat taccaacttg tctggtgtca aaaataatag gcctactagt cggccgtacg
     1081 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1141 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     1201 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     1261 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     1321 gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1381 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1441 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1501 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1561 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1621 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1681 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1741 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1801 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1861 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1921 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1981 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2041 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2101 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2161 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2221 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2281 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2341 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2401 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2461 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2521 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2581 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2641 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2701 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2761 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2821 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2881 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2941 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3001 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3061 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3121 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3181 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3241 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3301 cgcagcgagt cagtgagcga ggaagcggaa g
//
