LOCUS       40924_48573        3330 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing ZsGreen in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..981
                     /codon_start=1
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIADYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     promoter        1425..1529
                     /label=AmpR promoter
     CDS             1530..2387
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2561..3149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggctcagtca
      301 aagcacggtc taacaaaaga aatgacaatg aaataccgta tggaagggtg cgtcgatgga
      361 cataaatttg tgatcacggg agagggcatt ggatatccgt tcaaagggaa acaggctatt
      421 aatctgtgtg tggtcgaagg tggaccattg ccatttgccg aagacatatt gtcagctgcc
      481 tttatgtacg gaaacagggt tttcactgaa tatcctcaag acatagctga ctatttcaag
      541 aactcgtgtc ctgctggtta tacatgggac aggtcttttc tctttgagga tggagcagtt
      601 tgcatatgta atgcagatat aacagtgagt gttgaagaaa actgcatgta tcatgagtcc
      661 aaattttatg gagtgaattt tcctgctgat ggacctgtga tgaaaaagat gacagataac
      721 tgggagccat cctgcgagaa gatcatacca gtacctaagc aggggatatt gaaaggggat
      781 gtctccatgt acctccttct gaaggatggt gggcgtttac ggtgccaatt cgacacagtt
      841 tacaaagcaa agtctgtgcc aagaaagatg ccggactggc acttcatcca gcataagctc
      901 acccgtgaag accgcagcga tgctaagaat cagaaatggc atctgacaga acatgctatt
      961 gcatccggat ctgcattgcc ctgagcggcc gcgactctag aattccaact gagcgccggt
     1021 cgctaccatt accaacttgt ctggtgtcaa aaataatagg cctactagtc ggccgtacgg
     1081 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     1141 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     1201 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     1261 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1321 catcaggcgg ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1381 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2701 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3301 gcagcgagtc agtgagcgag gaagcggaag
//
