LOCUS       V001852                 5868 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001852
VERSION     V001852
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5868)
  AUTHORS   Liu J, Jonebring A, Hagstrom J, Nystrom AC, Lovgren A.
  TITLE     Improved Expression of Recombinant Human Factor IX by Co-expression
            of GGCX, VKOR and Furin
  JOURNAL   Protein J. 33 (2), 174-183 (2014)
   PUBMED   24567122
REFERENCE   2  (bases 1 to 5868)
  AUTHORS   Liu J, Jonebring A, Hagstrom J, Nystrom A-C., Lovgren A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2013) Astrazeneca R
REFERENCE   3  (bases 1 to 5868)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5868)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            J."; date: "2014"; volume: "33"; issue: "2"; pages: "174-183"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2013) Astrazeneca R"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..351
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        401..419
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             456..2837
                     /gene="FURIN"
                     /label="Furin"
                     /note="Furin from Homo sapiens. Accession#: P09958"
     polyA_signal    2919..3143
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3226..3814)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      4169..4624
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     enhancer        4660..4963
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4964..5167
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5285..5332
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5351..5722
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5739..5868
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatcgatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
       61 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctctct
      361 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      421 agacccaagc tggctagcgt ttaaacttaa gcaccatgga gctgaggccc tggttgctat
      481 gggtggtagc agcaacagga accttggtcc tgctagcagc tgatgctcag ggccagaagg
      541 tcttcaccaa cacgtgggct gtgcgcatcc ctggaggccc agcggtggcc aacagtgtgg
      601 cacggaagca tgggttcctc aacctgggcc agatcttcgg ggactattac cacttctggc
      661 atcgaggagt gacgaagcgg tccctgtcgc ctcaccgccc gcggcacagc cggctgcaga
      721 gggagcctca agtacagtgg ctggaacagc aggtggcaaa gcgacggact aaacgggacg
      781 tgtaccagga gcccacagac cccaagtttc ctcagcagtg gtacctgtct ggtgtcactc
      841 agcgggacct gaatgtgaag gcggcctggg cgcagggcta cacagggcac ggcattgtgg
      901 tctccattct ggacgatggc atcgagaaga accacccgga cttggcaggc aattatgatc
      961 ctggggccag ttttgatgtc aatgaccagg accctgaccc ccagcctcgg tacacacaga
     1021 tgaatgacaa caggcacggc acacggtgtg cgggggaagt ggctgcggtg gccaacaacg
     1081 gtgtctgtgg tgtaggtgtg gcctacaacg cccgcattgg aggggtgcgc atgctggatg
     1141 gcgaggtgac agatgcagtg gaggcacgct cgctgggcct gaaccccaac cacatccaca
     1201 tctacagtgc cagctggggc cccgaggatg acggcaagac agtggatggg ccagcccgcc
     1261 tcgccgagga ggccttcttc cgtggggtta gccagggccg aggggggctg ggctccatct
     1321 ttgtctgggc ctcggggaac gggggccggg aacatgacag ctgcaactgc gacggctaca
     1381 ccaacagtat ctacacgctg tccatcagca gcgccacgca gtttggcaac gtgccgtggt
     1441 acagcgaggc ctgctcgtcc acactggcca cgacctacag cagtggcaac cagaatgaga
     1501 agcagatcgt gacgactgac ttgcggcaga agtgcacgga gtctcacacg ggcacctcag
     1561 cctctgcccc cttagcagcc ggcatcattg ctctcaccct ggaggccaat aagaacctca
     1621 catggcggga catgcaacac ctggtggtac agacctcgaa gccagcccac ctcaatgcca
     1681 acgactgggc caccaatggt gtgggccgga aagtgagcca ctcatatggc tacgggcttt
     1741 tggacgcagg cgccatggtg gccctggccc agaattggac cacagtggcc ccccagcgga
     1801 agtgcatcat cgacatcctc accgagccca aagacatcgg gaaacggctc gaggtgcgga
     1861 agaccgtgac cgcgtgcctg ggcgagccca accacatcac tcggctggag cacgctcagg
     1921 cgcggctcac cctgtcctat aatcgccgtg gcgacctggc catccacctg gtcagcccca
     1981 tgggcacccg ctccaccctg ctggcagcca ggccacatga ctactccgca gatgggttta
     2041 atgactgggc cttcatgaca actcattcct gggatgagga tccctctggc gagtgggtcc
     2101 tagagattga aaacaccagc gaagccaaca actatgggac gctgaccaag ttcaccctcg
     2161 tactctatgg caccgcccct gaggggctgc ccgtacctcc agaaagcagt ggctgcaaga
     2221 ccctcacgtc cagtcaggcc tgtgtggtgt gcgaggaagg cttctccctg caccagaaga
     2281 gctgtgtcca gcactgccct ccaggcttcg ccccccaagt cctcgatacg cactatagca
     2341 ccgagaatga cgtggagacc atccgggcca gcgtctgcgc cccctgccac gcctcatgtg
     2401 ccacatgcca ggggccggcc ctgacagact gcctcagctg ccccagccac gcctccttgg
     2461 accctgtgga gcagacttgc tcccggcaaa gccagagcag ccgagagtcc ccgccacagc
     2521 agcagccacc tcggctgccc ccggaggtgg aggcggggca acggctgcgg gcagggctgc
     2581 tgccctcaca cctgcctgag gtggtggccg gcctcagctg cgccttcatc gtgctggtct
     2641 tcgtcactgt cttcctggtc ctgcagctgc gctctggctt tagttttcgg ggggtgaagg
     2701 tgtacaccat ggaccgtggc ctcatctcct acaaggggct gccccctgaa gcctggcagg
     2761 aggagtgccc gtctgactca gaagaggacg agggccgggg cgagaggacc gcctttatca
     2821 aagaccagag cgccctctag aacccgggat ccgatatcca gcacagtggc ggccgctcga
     2881 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     2941 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3001 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3061 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3121 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagcatgt gagcaaaagg
     3181 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3241 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3301 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3361 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3421 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3481 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3541 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3601 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3661 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3721 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3781 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3841 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga cattaaccta
     3901 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3961 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4021 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4081 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     4141 atgcgtaagg agaaaatacc gcatcaggac gcgccctgta gcggcgcatt aagcgcggcg
     4201 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4261 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4381 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4441 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4501 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4561 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4621 atttccattc gccattcagg ctgaactaga tctagagtcc gttacataac ttacggtaaa
     4681 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4741 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4801 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4861 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4921 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     4981 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     5041 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     5101 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     5161 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     5221 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgga
     5281 ccgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     5341 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     5401 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     5461 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     5521 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     5581 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     5641 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     5701 cttcgtggcc gaggagcagg actgacactc gacctcgaaa cttgtttatt gcagcttata
     5761 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5821 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtct
//
