LOCUS       .                       3805 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    9..25
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     terminator      123..209
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(747..1694)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1742..1964)
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2456..2550)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2622..3410)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
ORIGIN
        1 ctcgagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atcagcagga cgcactgacc aagcttatta aagaggagaa aggtacctga ctctagaggc
      121 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      181 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctagggt acgggttttg
      241 ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc agatccggct
      301 tcaggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg
      361 gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt
      421 tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat
      481 gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat
      541 ctgttacatt gtcgatctgt tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa
      601 gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct
      661 ttgatatcta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga
      721 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt
      781 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcatg cttactttgc
      841 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg
      901 tgtagtgttt ttcttagtcc gttacgtagg taggaatctg atgtaatggt tgttggtatt
      961 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta
     1021 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat
     1081 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc
     1141 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta
     1201 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa
     1261 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat
     1321 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg
     1381 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc
     1441 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca
     1501 ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt
     1561 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc
     1621 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca
     1681 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga
     1741 tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat ctgtaaattc
     1801 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga
     1861 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa
     1921 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt
     1981 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa
     2041 aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt
     2101 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct
     2161 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc
     2221 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc
     2281 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt
     2341 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca
     2401 gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg cttggattct
     2461 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2521 aggtcattac tggatctatc aacaggagtc caagcgagct ccggaattca gttaagccgc
     2581 gccgcgaagc ggcgtcggct tgaacgaatt gttagacatt atttgccgac taccttggtg
     2641 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     2701 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     2761 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     2821 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     2881 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     2941 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     3001 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     3061 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     3121 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     3181 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     3241 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     3301 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     3361 actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac
     3421 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     3481 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     3541 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     3601 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa
     3661 caggcttatg tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     3721 acctcagctg gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3781 tatcacgagg ccctttcgtc ttcac
//
