LOCUS       40924_48563        3230 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZRM2059, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Metabolically independent and accurately adjustable Aspergillus sp. 
            expression system
  JOURNAL   Appl. Environ. Microbiol. 71 (2), 672-678 (2005)
  PUBMED    15691916
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2004) Department of Applied Plant Sciences and 
            Plant Biotechnology, BOKU - University of Natural Resources and 
            Applied Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria
REFERENCE   3  (bases 1 to 3230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "2"; pages:
            "672-678"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JUN-2004) Department of Applied Plant Sciences and Plant 
            Biotechnology, BOKU - University of Natural Resources and Applied 
            Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(695..788)
                     /label=Aspergillus nidulans nirA promoter fragment
                     /note="Aspergillus nidulans nirA promoter fragment"
     misc_feature    complement(791..907)
                     /label=Saccharomyces cerevisiae URA3 promoter fragment
                     /note="Saccharomyces cerevisiae URA3 promoter fragment"
     misc_feature    complement(908..946)
                     /label=3xERE (estrogen responsive element)
                     /note="3xERE (estrogen responsive element)"
     primer_bind     complement(999..1015)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1045..1063)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1084..1100)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1108..1124)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1132..1162)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1177..1198)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1486..2074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2248..3105)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3106..3210)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atccatggta aatcaagccc agacagatat
      721 gttcggtgac agcgacagac caagacagcg catgcttatc ggaggaggac gctcggcgtg
      781 tttccctgca ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt
      841 cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt
      901 ccttctgggt cactgtgacc ggtcactgtg accggtcact gtgaccatgg aagctatcgt
      961 ccaactgcat ggagatctga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     1021 gggcccggta cccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc
     1081 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     1141 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     1201 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     1261 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1321 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1381 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1441 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1501 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1561 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1621 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1681 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1741 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1801 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1861 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1921 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1981 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2041 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2101 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2161 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2221 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2341 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
//
